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EpiQuik Global Histone H3 Acetylation Assay Kit

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Suggested Workflow
Histone Protein Extraction
 
 
Histone H3 Acetylation Quantification
 
Schematic procedure for using the EpiQuik Global Histone H3 Acetylation Assay Kit.
Acetylated histone H3 control was bound to microplate wells at different concentrations and measured using the EpiQuikâ„¢ Global Histone H3 Acetylation Assay Kit.
Input Type: Histone Extracts
Research Area: Histone Acetylation
Target Application: Amount Quantitation
Vessel Format: 96-Well Plate
100% Guarantee: 6 months
Catalog No.SizePriceQty
P-4008-4848 assays $338.00 
P-4008-9696 assays $499.00 
Availability: Usually Ships In 1-2 Days 
Product Overview

The EpiQuik™ Global Histone H3 Acetylation Assay Kit is a convenient package of tools that allows the experimenter to measure global acetylation of histone H3 at tremendously fast speeds and consistency, superior and safer than all other current methods. The kit is ready-to-use and provides all the essential components needed to carry out a successful assay experiment. The EpiQuik™ Global Histone H3 Acetylation Assay Kit is suitable for specifically measuring global histone H3 acetylation using a variety of mammalian cells including fresh and frozen tissues, and cultured adherent and suspension cells. The kit has the following advantages:

  • Quick and efficient procedure, which can be finished within 5 hours.
  • Innovative colorimetric assay without the need for radioactivity, electrophoresis, or chromatography.
  • Strip microplate format makes the assay flexible: manual or high throughput analysis.
  • Simple, reliable, and consistent assay conditions.

Principle & Procedure
The EpiQuik™ Global Histone H3 Acetylation Assay Kit is designed for measuring global histone H3 acetylation. In an assay with this kit, the histone proteins are stably spotted on the strip wells. The acetylated histone H3 can be recognized with a high-affinity antibody. The ratio or amount of acetylated histone H3 can be quantified through HRP conjugated secondary antibody-color development system and is proportional to the intensity of color development.

Product Components

GF1 (10X lysis buffer)
GF2 (extraction buffer)
GF3 (10X Wash Buffer)
GF4 (Histone Buffer)
GF5 (Blocking Buffer)
GF6 (Antibody Buffer)
GF7 (Capture Antibody, 100 µg/ml)*
GF8 (Detection Antibody, 400 µg/ml)*
GF9 (Developing Solution)
GF10 (Stop Solution)
Acetylated Histone H3 Control (60 µg/ml)*
8-Well Assay Strips (with Frame)
User Guide

* For maximum recovery of the products, centrifuge the original vial prior to opening the cap.

User Guide & MSDS

[User Guide]*
*Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing info@epigentek.com along with your contact information and institution name.

[Material Safety Data Sheet]
Product Citations

Bilan Li et. al. (March 2017). DICER1 regulates endometrial carcinoma invasion via histone acetylation and methylation Journal of Cancer. 8:933 -939.

Zhou K et. al. (February 2017). Aberrant histone modification in CD19<sup>+</sup> B cells of patients with chronic lymphocytic leukemia. Onco Targets Ther. 10:1173-1179.

Bramble MS et. al. (November 2016). Sex-Specific Effects of Testosterone on the Sexually Dimorphic Transcriptome and Epigenome of Embryonic Neural Stem/Progenitor Cells. Sci Rep. 6:36916.

Li Y et. al. (August 2016). Combinatorial epigenetic mechanisms and efficacy of early breast cancer inhibition by nutritive botanicals. Epigenomics. 8(8):1019-37.

Sramek M et. al. (February 2016). Non-DHFR-mediated effects of methotrexate in osteosarcoma cell lines: epigenetic alterations and enhanced cell differentiation. Cancer Cell Int. 16:14.

Sharma S et. al. (January 2016). Epigenetic modifications by inhibiting histone deacetylases reverse memory impairment in insulin resistance induced cognitive deficit in mice. Neuropharmacology. 105:285-297.

Liu XY et. al. (December 2015). Reduced Histone H3 Acetylation in CD4(+) T Lymphocytes: Potential Mechanism of Latent Autoimmune Diabetes in Adults. Dis Markers. 2015:285125.

Xi-yu Liu and Jiang-feng Xu et. al. (December 2015). Reduced Histone H3 Acetylation in CD4+ T Lymphocytes: Potential Mechanism of Latent Autoimmune Diabetes in Adults Disease Markers.

Yan N et. al. (June 2015). Histone hypoacetylation and increased histone deacetylases in peripheral blood mononuclear cells from patients with Graves' disease. Mol Cell Endocrinol.

Novohradsky V et. al. (June 2015). New insights into the molecular and epigenetic effects of antitumor Pt(IV)-valproic acid conjugates in human ovarian cancer cells. Biochem Pharmacol. 95(3):133-44.

Yang Y et. al. (March 2015). The effect of mycophenolic acid on epigenetic modifications in lupus CD4<sup>+</sup>T cells. Clin Immunol.

Sutendra G et. al. (July 2014). A nuclear pyruvate dehydrogenase complex is important for the generation of acetyl-CoA and histone acetylation. Cell. 158(1):84-97.

Kumar P et. al. (June 2014). All-trans retinoic acid and sodium butyrate enhance natriuretic peptide receptor a gene transcription: role of histone modification. Mol Pharmacol. 85(6):946-57.

Kumar P et. al. (March 2014). Histone deacetylase inhibitors modulate the transcriptional regulation of guanylyl cyclase/natriuretic peptide receptor-a gene: interactive roles of modified histones, histone acetyltransferase, p300, AND Sp1. J Biol Chem. 289(10):6991-7002.

Cho Y et. al. (March 2014). Colon cancer cell apoptosis is induced by combined exposure to the n-3 fatty acid docosahexaenoic acid and butyrate through promoter methylation. Exp Biol Med (Maywood). 239(3):302-10.

Monteiro JB et. al. (March 2014). Endometriosis is characterized by a distinct pattern of histone 3 and histone 4 lysine modifications. Reprod Sci. 21(3):305-18.

Habib O et. al. (December 2013). An improved method for the derivation of high quality iPSCs in the absence of c-Myc. Exp Cell Res. 319(20):3190-200.

Doherty R et. al. (August 2013). Epigenetic regulation of the innate immune response to LPS in bovine peripheral blood mononuclear cells (PBMC). Vet Immunol Immunopathol. 154(3-4):102-10.

Xiaomeng X et. al. (March 2013). Aberrant histone acetylation and methylation levels in woman with endometriosis. Arch Gynecol Obstet. 287(3):487-94.

Rehan VK et. al. (October 2012). Perinatal nicotine exposure induces asthma in second generation offspring. BMC Med. 10:129.

Ghadimi D et. al. (October 2012). Epigenetic imprinting by commensal probiotics inhibits the IL-23/IL-17 axis in an in vitro model of the intestinal mucosal immune system. J Leukoc Biol. 92(4):895-911.

Easley CA 4th et. al. (June 2012). Human amniotic epithelial cells are reprogrammed more efficiently by induced pluripotency than adult fibroblasts. Cell Reprogram. 14(3):193-203.

Zhao M et. al. (February 2012). Abnormal epigenetic modifications in peripheral blood mononuclear cells from patients with alopecia areata. Br J Dermatol. 166(2):226-73.

Sanchez-Roman I et. al. (December 2011). Forty percent methionine restriction lowers DNA methylation, complex I ROS generation, and oxidative damage to mtDNA and mitochondrial proteins in rat heart. J Bioenerg Biomembr. 43(6):699-708.

Zhang Z et. al. (October 2011). Epigenetic suppression of GAD65 expression mediates persistent pain. Nat Med. 17(11):1448-55.

Chen C et. al. (October 2011). Abnormal histone acetylation and methylation levels in esophageal squamous cell carcinomas. Cancer Invest. 29(8):548-56.

Kraft M et. al. (September 2011). Disruption of the histone acetyltransferase MYST4 leads to a Noonan syndrome-like phenotype and hyperactivated MAPK signaling in humans and mice. J Clin Invest. 121(9):3479-91.

Rivière G et. al. (April 2011). Epigenetic regulation of somatic angiotensin-converting enzyme by DNA methylation and histone acetylation. Epigenetics. 6(4):478-89.

Sui L et. al. (December 2010). Effects of perinatal hypothyroidism on regulation of reelin and brain-derived neurotrophic factor gene expression in rat hippocampus: Role of DNA methylation and histone acetylation. Steroids. 75(12):988-97.

Kobori A et. al. (May 2010). Butyrate stimulates IL-32alpha expression in human intestinal epithelial cell lines. World J Gastroenterol. 16(19):2355-61.

Nicolai J et. al. (April 2010). CXCL12 inhibits expression of the NMDA receptor's NR2B subunit through a histone deacetylase-dependent pathway contributing to neuronal survival. Cell Death Dis. 1:e33.

Biermann J et. al. (January 2010). Valproic acid-mediated neuroprotection and regeneration in injured retinal ganglion cells. Invest Ophthalmol Vis Sci. 51(1):526-34.

Hu N et. al. (November 2009). Aberrant expression pattern of histone acetylation modifiers and mitigation of lupus by SIRT1-siRNA in MRL/lpr mice. Scand J Rheumatol. 38(6):464-71.

Hu N et. al. (May 2008). Abnormal histone modification patterns in lupus CD4+ T cells. J Rheumatol. 35(5):804-10.

Tsuchiya N et. al. (December 2004). Association of a functional CD19 polymorphism with susceptibility to systemic sclerosis. Arthritis Rheum. 50(12):4002-7.

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