The EpiQuik™ Global Histone H3 Acetylation Assay Kit is a convenient package of tools that allows the experimenter to measure global acetylation of histone H3 at tremendously fast speeds and consistency, superior and safer than all other current methods. The kit is ready-to-use and provides all the essential components needed to carry out a successful assay experiment. The EpiQuik™ Global Histone H3 Acetylation Assay Kit is suitable for specifically measuring global histone H3 acetylation using a variety of mammalian cells including fresh and frozen tissues, and cultured adherent and suspension cells.

WHY CHOOSE THE EPIQUIK™ GLOBAL HISTONE H3 ACETYLATION ASSAY KIT?

• Quick and efficient procedure, which can be finished within 5 hours.
• Innovative colorimetric assay without the need for radioactivity, electrophoresis, or chromatography.
• Strip microplate format makes the assay flexible: manual or high throughput analysis.
• Simple, reliable, and consistent assay conditions.

The EpiQuik™ Global Histone H3 Acetylation Assay Kit is designed for measuring global histone H3 acetylation. In an assay with this kit, the histone proteins are stably spotted on the strip wells. The acetylated histone H3 can be recognized with a high-affinity antibody. The ratio or amount of acetylated histone H3 can be quantified through HRP conjugated secondary antibody-color development system and is proportional to the intensity of color development.

SCHEMATIC PROCEDURE:

GF1 (10X lysis buffer)
GF2 (extraction buffer)
GF3 (10X Wash Buffer)
GF4 (Histone Buffer)
GF5 (Blocking Buffer)
GF6 (Antibody Buffer)
GF7 (Capture Antibody, 100 µg/ml)*
GF8 (Detection Antibody, 400 µg/ml)*
GF9 (Developing Solution)
GF10 (Stop Solution)
Acetylated Histone H3 Control (60 µg/ml)*
8-Well Assay Strips (with Frame)
User Guide

* For maximum recovery of the products, centrifuge the original vial prior to opening the cap.

[User Guide]* *Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing info@epigentek.com along with your contact information and institution name.

[Material Safety Data Sheet]

Sanchez-Roman, I. et. al. (December 2011). Forty percent methionine restriction lowers DNA methylation, complex I ROS generation, and oxidative damage to mtDNA and mitochondrial proteins in rat heart. J Bioenerg Biomembr. 43(6):699-708. PubMed Abstract

Zhang, Z. et. al. (October 2011). Epigenetic suppression of GAD65 expression mediates persistent pain. Nat Med. 17(11):1448- 55. PubMed Abstract

Chen, C. et. al. (October 2011). Abnormal histone acetylation and methylation levels in esophageal squamous cell/ carcinomas. Cancer Invest. 29(8):548-56. PubMed Abstract

Kraft, M. et. al. (September 2011). Disruption of the histone acetyltransferase MYST4 leads to a Noonan syndrome–like phenotype and hyperactivated MAPK signaling in humans and mice. J Clin Invest. 121(9):3479-3491. PubMed Abstract

Rivière, G. et. al. (April 2011). Epigenetic regulation of somatic angiotensin-converting enzyme by DNA methylation and histone acetylation. Epigenetics 6(4):478-89. PubMed Abstract

Sui, L. et. al. (June 2010). Effects of perinatal hypothyroidism on regulation of reelin and brain-derived neurotrophic factor gene expression in rat hippocampus: Role of DNA methylation and histone acetylation. Steroids. Epub ahead of print. PubMed Abstract

Kobori, A. et. al. (May 2010). Butyrate stimulates IL-32alpha expression in human intestinal epithelial cell lines. World Journal of Gastroenterology. 16(19): 2355-2361. PubMed Abstract

Nicolai, J. et. al. (April 2010). CXCL12 inhibits expression of the NMDA receptor's NR2B subunit through a histone deacetylase-dependent pathway contributing to neuronal survival. Cell Death and Disease. 1: e33. Full PDF Article

Biermann, J. et. al. (January 2010). Valproic acid-mediated neuroprotection and regeneration in injured retinal ganglion cells. Invest Ophthalmol Vis Sci. 51(1): 526-34. PubMed Abstract

Hu, N. et. al. (December 2009). Aberrant expression pattern of histone acetylation modifiers and mitigation of lupus by SIRT1-siRNA in MRL/lpr mice. Scand J Rheumatol. 38(6): 464-471. PubMed Abstract

Hu, N. et. al. (May 2008). Abnormal histone modification patterns in lupus CD4+ T cells. J Rheumatol. 35(5): 804-10. PubMed Abstract