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HDAC4 Polyclonal Antibody

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Western blot analysis of extracts of various cell lines, using HDAC4 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Immunofluorescence analysis of A549 cells using HDAC4 antibody. Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug HDAC4 antibody. Western blot was performed from the immunoprecipitate using HDAC4 antibody at a dilition of 1:1000.
Application: IF, WB
Areas of Research: Histone Acetylation & Deacetylation
Clonality: Polyclonal
Conjugate: Unconjugated
Host: Rabbit
Isotype: IgG
Purification: Affinity Purified
Reactivity: Human, Mouse, Rat
100% Guarantee: 6 months
Catalog No.SizePriceQty
A-4004-05050 µl $199.00 
A-4004-100100 µl $391.00 
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Product Overview

Background
Histone deacetylases (HDACs) play a critical role in transcriptional repression of geneexpression in eukaryotic cells through catalyzing the hydrolytic removal of acetylgroups from histone lysine residues. Three distinct families of HDACs have beendescribed, comprising a group of at least 20 proteins in humans. HDAC4 is a class I histone deacetylase.

Description
HDAC4 Polyclonal Antibody. Unconjugated. Raised in: Rabbit.

Formulation
Liquid. PBS with 0.02% sodium azide, 50% glycerol, pH7.3

Specificity
Mouse, Rat, Human

Isotype
IgG

Purification
Affinity Purified

Immunogen
Synthetic Peptide of Human HDAC4

Storage
Shipped at 4°C. Store at -20°C. Avoid multiple freeze/thaw cycles.

Alternative Names
AHO3 antibody, BDMR antibody, EC 3.5.1.98 antibody, HA6116 antibody, HD 4 antibody, HDACA antibody, Histone Deacetylase 4 antibody, Histone Deacetylase A antibody, KIAA0288 antibody

Application
WB, IF; Recommended dilution: WB 1:500 - 1:2000, IF 1:50 - 1:200, IP 1:50 - 1:100

User Guide & MSDS

[User Guide]*
*Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing info@epigentek.com along with your contact information and institution name.

[Safety Data Sheet]
Product Citations

Pitcher J et. al. (February 2014). Neuronal ferritin heavy chain and drug abuse affect HIV-associated cognitive dysfunction. J Clin Invest. 124(2):656-69.

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