Bai Z et. al. (March 2023). Inhibition of IL-6 methylation by Saikosaponin C regulates neuroinflammation to alleviate depression.Int Immunopharmacol. 118:110043. The research investigates the potential of Saikosaponin C (SSc) to alleviate depressive symptoms in mice caused by chronic social defeat stress (CSDS) by regulating neuroinflammation. The study shows that SSc reduces IL-6 methylation, downregulates inflammatory cytokines, increases dendritic spine density, and promotes synaptic plasticity in mice. It also suggests that SSc may potentially treat depression and other neurodegenerative diseases in humans.
(Products Used: EpiQuik Nuclear Extraction Kit)
Du Y et. al. (March 2023). Sufficient coumarin accumulation improves apple resistance to Cytospora mali under high potassium status.Plant Physiol. The paper discusses the mechanisms behind how adding potassium to apple trees can improve their resistance to Cytospora canker. The study found that high potassium levels in apple tissues led to upregulation of resistance genes, callose deposition, and the formation of ligno-suberized tissues, which collectively contributed to higher disease resistance. The study also identified that the phenylpropanoid pathway was reprogrammed under high-K conditions, leading to the accumulation of antifungal chemicals.
(Products Used: EpiQuik Plant ChIP Kit)
Fuchs H et. al. (March 2023). The chances in the redox priming of nondormant recalcitrant seeds by spermidine.Tree Physiol. The study investigates the effect of spermidine (Spd) treatment on nondormant recalcitrant (desiccation sensitive) seeds of the silver maple to improve their postharvest performance. The researchers found that Spd treatment improved antioxidative capacity, cellular membrane stability, protected genome integrity from oxidative damage, and increased the efficiency of mitochondria. However, they also observed a hydrogen peroxide burst and suggested that redox priming may not be a simple solution to reinvigorate stored seeds with low-efficiency antioxidant systems.
(Products Used: EpiQuik 8-OHdG DNA Damage Quantification Direct Kit (Colorimetric))
Wang Y et. al. (March 2023). METTL3-mediated m6A modification of HMGA2 mRNA promotes subretinal fibrosis and epithelial-mesenchymal transition.J Mol Cell Biol. The research investigates the role of N6-methyladenosine (m6A) modification in the development of subretinal fibrosis, a major cause of poor visual prognosis in patients with neovascular age-related macular degeneration (nAMD). Through mouse models and verification, the study shows that the METTL3-mediated m6A modification of high mobility group AT-hook 2 (HMGA2) mRNA promotes subretinal fibrosis and epithelial-mesenchymal transition (EMT) in retinal pigment epithelial (RPE) cells, highlighting a potential therapeutic target for nAMD.
(Products Used: EpiQuik m6A RNA Methylation Quantification Kit (Colorimetric))