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Jeffrey MP et. al. (April 2024). A Lacticaseibacillus rhamnosus secretome induces immunoregulatory transcriptional, functional and immunometabolic signatures in human THP-1 monocytes Sci Rep. 14(1):8379.
In this study, researchers investigated long-lived proteins (LLPs) and DNA as potential predictive biomarkers for tissue-associated diseases. Using in vivo stable isotope labeling in mice, they identified 2113 LLPs in ten tissues and plasma, including both widespread and tissue-specific proteins. A significant percentage of these LLPs were detected in plasma, suggesting a potential link to age-related cardiovascular diseases. LLPs identified in the brain were related to neurodegenerative diseases. Additionally, the study showed that the relative quantification of DNA-derived deoxynucleosides from the same tissues correlated well with LLPs in the brain, providing insight into cellular DNA renewal and potential pathology. These findings suggest that tissue-derived peripheral LLPs could serve as biomarkers for aging and age-related diseases.
Products Used: MethylFlash Methylated DNA 5-mC Quantification Kit (Colorimetric), EpiQuik Global Histone H3 Acetylation Assay Kit, EpiQuik Global Histone H4 Acetylation Assay Kit
Liu X et. al. (April 2024). Long-lived proteins and DNA as candidate predictive biomarkers for tissue associated diseases iScience. 27(4):109642.
In this study, researchers identified long-lived proteins (LLPs) and DNA as potential biomarkers for tissue-associated diseases. Using stable isotope labeling in mice, they found 2113 LLPs in ten tissues and plasma, including those related to age-related cardiovascular and neurodegenerative diseases. The study suggests that tissue-derived peripheral LLPs could be biomarkers for aging and age-related diseases.
Products Used: FitAmp General Tissue Section DNA Isolation Kit, EpiQuik One-Step DNA Hydrolysis Kit
Yuan Q et. al. (April 2024). DNMT1/miR-152-3p/SOS1 signaling axis promotes self-renewal and tumor growth of cancer stem-like cells derived from non-small cell lung cancer Clin Epigenetics. 16(1):55.
This study examined the DNMT1/miR-152-3p/SOS1 signaling axis in regulating the self-renewal and tumor growth of lung cancer stem-like cells (LCSLCs). The findings revealed a negative interaction between DNMT1 and miR-152-3p, leading to the activation of SOS1 and promoting self-renewal and tumor growth of LCSLCs. The study suggests that targeting this axis could be a potential therapeutic strategy for non-small cell lung cancer (NSCLC) treatment.
Products Used: EpiQuik Nuclear Extraction Kit, EpiQuik DNMT Activity/Inhibition Assay Ultra Kit (Colorimetric)
Roh JD et. al. (April 2024). Placental senescence pathophysiology is shared between peripartum cardiomyopathy and preeclampsia in mouse and human Sci Transl Med. 16(743):eadi0077.
The article investigates the shared pathophysiology of peripartum cardiomyopathy (PPCM) and preeclampsia, focusing on placental senescence. Using serum proteomics, researchers identified the senescence-associated secretory phenotype (SASP) as upregulated in women with PPCM or preeclampsia. Placentas from women with preeclampsia showed markers of senescence and increased expression of SASP-related proteins. Inhibiting activin A, a key SASP factor, improved heart function in a murine PPCM model, suggesting a potential therapeutic target.
Products Used: MethylFlash Global DNA Methylation (5-mC) ELISA Easy Kit (Colorimetric)
Mauceri A et. al. (April 2024). Integrated omics approach reveals the molecular pathways activated in tomato by Kocuria rhizophila, a soil plant growth-promoting bacterium Plant Physiol Biochem. 210:108609.
This study investigates the molecular pathways activated in tomato plants by the soil bacterium Kocuria rhizophila, known for its plant growth-promoting abilities. Using a multi-omics approach, the research sheds light on how K. rhizophila enhances plant growth, revealing upregulation of genes related to amino acid metabolism, cell wall organization, and lipid and secondary metabolism. The study also highlights changes in DNA methylation, accumulation of proteins involved in photosynthesis and cell division, and modulation of key metabolites like amino acids and sugars.
Products Used: MethylFlash Methylated DNA Quantification Kit (Fluorometric)
Li J et. al. (April 2024). ALKBH5-mediated m6A demethylation of pri-miR-199a-5p exacerbates myocardial ischemia/reperfusion injury by regulating TRAF3-mediated pyroptosis J Biochem Mol Toxicol. 38(4):e23710.
This article explores the role of alkB homolog 5 (ALKBH5) in myocardial ischemia‒reperfusion injury (MI/RI) and its association with pyroptosis. Using rat and cell models, the research shows that ALKBH5 is overexpressed in MI/RI, and its silencing reduces injury and inhibits pyroptosis. ALKBH5 mediates the demethylation of pri-miR-199a-5p, inhibiting its maturation and expression. This leads to increased expression of TNF receptor-associated Factor 3 (TRAF3), a downstream target of miR-199a-5p, ultimately exacerbating pyroptosis in MI/RI.
Products Used: EpiQuik m6A RNA Methylation Quantification Kit (Colorimetric)
Fan Y et. al. (April 2024). Cancer-associated fibroblasts-derived exosomal METTL3 promotes the proliferation, invasion, stemness and glutaminolysis in non-small cell lung cancer cells by eliciting SLC7A5 m6A modification Hum Cell.
This study explores how cancer-associated fibroblasts (CAFs) promote non-small cell lung cancer (NSCLC) progression through exosomal METTL3-mediated N6-methyladenine (m6A) modification. Functional assays demonstrate that CAFs enhance NSCLC cell proliferation, invasion, stemness, and glutaminolysis. METTL3, enriched in CAFs and packaged into exosomes, increases m6A levels in NSCLC cells. Exosomal METTL3 induces m6A modification in Amino acid transporter LAT1 (SLC7A5) mRNA, stabilizing its expression in NSCLC cells and promoting cancer progression. These findings highlight a novel mechanism of CAFs in driving NSCLC progression via exosomal METTL3-mediated m6A modification.
Products Used: EpiQuik m6A RNA Methylation Quantification Kit (Colorimetric)
Wu L et. al. (April 2024). Inhibition of METTL3 ameliorates doxorubicin-induced cardiotoxicity through suppression of TFRC-mediated ferroptosis Wu L et. al. (April 2024).
This article investigates the role of N6-methyladenosine (m6A) RNA modification and its methyltransferase METTL3 in doxorubicin-induced cardiotoxicity (DIC). Mice treated with doxorubicin show increased m6A modification and METTL3 expression in cardiomyocytes, mediated by c-Jun. Conditional knockout of METTL3 in cardiomyocytes improves cardiac function, remodeling, and injury in response to doxorubicin. METTL3 promotes m6A modification of TFRC mRNA, which governs iron uptake, leading to increased stability and expression of TFRC. Inhibition of METTL3 with a selective inhibitor attenuates DIC, suggesting a potential therapeutic strategy for managing doxorubicin-induced cardiotoxicity.
Products Used: EpiQuik m6A RNA Methylation Quantification Kit (Colorimetric)
Chu Y et. al. (April 2024). AtHD2D is involved in regulating lateral root development and participates in abiotic stress response in Arabidopsis J Plant Physiol. 297:154242.
This study investigates the role of the histone deacetylase AtHD2D in Arabidopsis root development and stress response. AtHD2D affects root tip microenvironment homeostasis and is involved in regulating lateral root development, possibly through auxin-mediated pathways. It also enhances Arabidopsis resistance to abiotic stress, possibly by promoting lateral root development and influencing ROS accumulation. These findings provide insights into the molecular mechanisms of AtHD2D in root development and stress responses.
Products Used: EpiQuik Total Histone Extraction Kit
