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EpiQuik One-Step DNA Hydrolysis Kit

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The EpiQuik™ One-Step DNA Hydrolysis Kit simply applies our proprietary enzymatic DNA digestion solution to DNA or oligonucleotides. After treatment with the DNA digestion buffer, DNA is easily digested into single nucleosides without phosphate groups.
0.5 ug and 2 ug of fully methylated HeLa DNA were treated with the kit at 37C for 1 h and then analyzed by gel electrophoresis. 1: 2 ug of undigested DNA; 2: 0.5 ug of digested DNA; 3: 2 ug of digested DNA; 4: 1 ug of deoxynucleoside; “M” is a 2 kb DNA ladder.
1 ug of fully methylated HeLa DNA was digested with the kit at 37C fro 1 and 2 h, respectively. The digested DNA was fluorescently quantified. 1: Blank; 2: Deoxynucleoside control; 3: undigested DNA; 4: undigested tri-oligos (3 nt); 5: undigested penta-oligos (5 nt); 6: DNA digested for 1 h; 7: DNA digested for 2 h.
Input Type: DNA
Research Area: DNA Damage & Repair, DNA Methylation
Target Application: Sample Isolation
Vessel Format: Columns/Tubes
100% Guarantee: 6 months
Catalog No.SizePriceQty
P-1023-9696 samples $162.00 
Availability: Usually Ships In 1-2 Days 
Product Overview

The EpiQuik™ One-Step DNA Hydrolysis Kit is a complete set of essential components which enables the experimenter to rapidly hydrolyze DNA to deoxynucleosides with a process that can be performed in a single incubation. In comparison to currently used methods for digesting DNA with tri-enzymes, which have several drawbacks and excessive time consumption, the EpiQuik™ One-Step DNA Hydrolysis Kit provides a highly efficient enzymatic hydrolysis in minimal time. It is also very suitable for digesting DNA from various biological materials. 

WHY CHOOSE THE EPIQUIK™ ONE-STEP DNA HYDROLYSIS KIT,

  • A fast one-step procedure, which can be finished in as short as 1 hour.
  • Performed in a single incubation, without the need for DNA denaturation.
  • Highly efficient enzymatic hydrolysis: non-specifically digests DNA or oligonucleotides into single nucleosides.
  • 96-well microplate format makes the sample preparation flexible for use with high throughput formats.

Fig. 1. The EpiQuik™ One-Step DNA Hydrolysis Kit simply applies our proprietary enzymatic DNA digestion solution to DNA or oligonucleotides. After treatment with the DNA digestion buffer, DNA is easily digested into single nucleosides without phosphate groups.

 

 

Fig. 2. 0.5 µg and 2 µg of fully methylated HeLa DNA were treated with the kit at 37C for 1 h and then analyzed by gel electrophoresis. 1: 2 ug of undigested DNA; 2: 0.5 ug of digested DNA; 3: 2 ug of digested DNA; 4: 1 ug of deoxynucleoside; “M” is a 2 kb DNA ladder. 

 


Fig. 3. 1 ug of fully methylated HeLa DNA was digested with the kit at 37C fro 1 and 2 h, respectively.  The digested DNA was fluorescently quantified.  1: Blank; 2: Deoxynucleoside control; 3: undigested DNA; 4: undigested tri-oligos (3 nt); 5: undigested penta-oligos (5 nt); 6:  DNA digested for 1 h; 7: DNA digested for 2 h.

 

 

Product Components

DH1 (Enzyme Mix)
DH2 (Digestion Enhancer)
DH3 (Digestion Buffer)
96-Well Microplate
Optical Adhesive Cover
User Guide

User Guide & MSDS

[User Guide]*
*Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing info@epigentek.com along with your contact information and institution name.

[Material Safety Data Sheet]
[Safety Data Sheet]
Product Citations

Sekar K et. al. (November 2018). Synthesis and degradation of FtsZ quantitatively predict the first cell division in starved bacteria. Mol Syst Biol. 14(11):e8623.

Aravind Kumar M et. al. (August 2018). Whole exome sequencing of breast cancer (TNBC) cases from India: association of MSH6 and BRIP1 variants with TNBC risk and oxidative DNA damage. Mol Biol Rep.

Sekar K et al. et. al. (May 2018). Synthesis and degradation of FtsZ determines the first cell division in starved bacteria bioRxiv.

Buxbaum NP et. al. (June 2017). In vivo kinetics and nonradioactive imaging of rapidly proliferating cells in graft-versus-host disease. JCI Insight. 2(12)

Wang XZ et. al. (October 2016). Peperomin E reactivates silenced tumor suppressor genes in lung cancer cells by inhibition of DNA methyltransferase. Cancer Sci. 107(10):1506-1519.

Wang XZ et. al. (October 2016). Peperomin E reactivates silenced tumor suppressor genes in lung cancer cells by inhibition of DNA methyltransferase. Cancer Sci. 107(10):1506-1519.

Raja M et. al. (March 2016). Liquid chromatography-mass spectrometry as a general approach for investigating covalent binding of drugs to DNA. Anal Bioanal Chem.

Wang J et. al. (March 2014). Genome-wide screen of DNA methylation changes induced by low dose X-ray radiation in mice. PLoS One. 9(3):e90804.

Farthing DE et. al. (May 2013). Sensitive GC-MS/MS method to measure deuterium labeled deoxyadenosine in DNA from limited mouse cell populations. Anal Chem. 85(9):4613-20.

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