The EpiQuik™ One-Step DNA Hydrolysis Kit is a complete set of essential components which enables the experimenter to rapidly hydrolyze DNA to deoxynucleosides with a process that can be performed in a single incubation. In comparison to currently used methods for digesting DNA with tri-enzymes, which have several drawbacks and excessive time consumption, the EpiQuik™ One-Step DNA Hydrolysis Kit provides a highly efficient enzymatic hydrolysis in minimal time. It is also very suitable for digesting DNA from various biological materials.
WHY CHOOSE THE EPIQUIK™ ONE-STEP DNA HYDROLYSIS KIT,
- A fast one-step procedure, which can be finished in as short as 1 hour.
- Performed in a single incubation, without the need for DNA denaturation.
- Highly efficient enzymatic hydrolysis: non-specifically digests DNA or oligonucleotides into single nucleosides.
- 96-well microplate format makes the sample preparation flexible for use with high throughput formats.
Fig. 1. The EpiQuik™ One-Step DNA Hydrolysis Kit simply applies our proprietary enzymatic DNA digestion solution to DNA or oligonucleotides. After treatment with the DNA digestion buffer, DNA is easily digested into single nucleosides without phosphate groups.
Fig. 2. 0.5 µg and 2 µg of fully methylated HeLa DNA were treated with the kit at 37C for 1 h and then analyzed by gel electrophoresis. 1: 2 ug of undigested DNA; 2: 0.5 ug of digested DNA; 3: 2 ug of digested DNA; 4: 1 ug of deoxynucleoside; “M” is a 2 kb DNA ladder.
Fig. 3. 1 ug of fully methylated HeLa DNA was digested with the kit at 37C fro 1 and 2 h, respectively. The digested DNA was fluorescently quantified. 1: Blank; 2: Deoxynucleoside control; 3: undigested DNA; 4: undigested tri-oligos (3 nt); 5: undigested penta-oligos (5 nt); 6: DNA digested for 1 h; 7: DNA digested for 2 h.