RNA Methylation Analysis Made Easy
Expand upon your understanding of RNA methylation with the help of EpiGentek’s complete suite of RNA methylation products. From directly quantifying the "fifth RNA base" N6-Methyladenosine (m6A) to measuring global 5-mC RNA and more, our kits make it easy for you to determine how RNA methylation influences your research project.
m6A RNA Methylation
Investigate N6-methyladenosine (m6A) -- the most common and abundant methylation modification in RNA molecules present in eukaryotes. Known as the "fifth RNA base", m6A RNA modifications are reversible, highly dynamic, and likely play important biological roles comparable to DNA methylation and histone modification.
For absorbance-based quantitation of N6-methyladenosine in RNA in an ELISA-like format
*Also available for DNA
EpiQuik m6A RNA Methylation Quantification Kit (Fluorometric)
For fluorescence-based quantitation of N6-methyladenosine in RNA in an ELISA-like format
MethylFlash Urine N6-methyladenosine (m6A) Quantification Kit (Colorimetric)
For absorbance-based quantitation of N6-methyladenosine in RNA from urine samples in an ELISA-like format
Epigenase m6A Demethylase Activity/Inhibition Assay Kit (Colorimetric)
For absorbance-based measurement of total m6A demethylases activity/inhibition using nuclear extracts or purified m6A demethylases
5-mC RNA Methylation
Study the occurrence of 5-methylcytosine (5-mC) in RNA -- a well-known methylation modification found in various RNA molecules including tRNAs, rRNAs, mRNAs and non-coding RNAs (ncRNAs). Evidence has shown that RNA cytosine methylation affects the regulation of various biological processes such as RNA stability and mRNA translation, and that loss of 5-mC in vault RNAs may cause aberrant processing into Argonaute-associated small RNA fragments that can function as microRNAs.