Expand upon your understanding of RNA methylation with the help of EpiGentek’s complete suite of RNA methylation products. From directly quantifying the "fifth RNA base" N6-Methyladenosine (m6A) to measuring global 5-mC RNA and more, our kits make it easy for you to determine how RNA methylation influences your research project.

Investigate N6-methyladenosine (m6A) -- the most common and abundant methylation modification in RNA molecules present in eukaryotes. Known as the "fifth RNA base", m6A RNA modifications are reversible, highly dynamic, and likely play important biological roles comparable to DNA methylation and histone modification.

• EpiQuik m6A RNA Methylation Quantification Kit (Colorimetric)
  For absorbance-based quantitation of N6-methyladenosine in RNA in an ELISA-like format
*Also available for DNA


• EpiQuik m6A RNA Methylation Quantification Kit (Fluorometric)
  For fluorescence-based quantitation of N6-methyladenosine in RNA in an ELISA-like format


• MethylFlash Urine N6-methyladenosine (m6A) Quantification Kit (Colorimetric)
  For absorbance-based quantitation of N6-methyladenosine in RNA from urine samples in an ELISA-like format


• Epigenase m6A Demethylase Activity/Inhibition Assay Kit (Colorimetric)
  For absorbance-based measurement of total m6A demethylases activity/inhibition using nuclear extracts or purified m6A demethylases
  
  
• Epigenase m6A Methylase Activity/Inhibition Assay Kit (Colorimetric)
  For absorbance-based measurement of total m6A methylases activity/inhibition from nuclear extracts or purified enzymes


• N6-methyladenosine (m6A) Polyclonal Antibody or m6A Monoclonal Antibody
  For antibody-based applications requiring high specificity to N6-methyladenosine (m6A)

Study the occurrence of 5-methylcytosine (5-mC) in RNA -- a well-known methylation modification found in various RNA molecules including tRNAs, rRNAs, mRNAs and non-coding RNAs (ncRNAs). Evidence has shown that RNA cytosine methylation affects various biological processes, such as RNA stability and mRNA translation. The loss of 5-mC in vault RNAs may cause aberrant processing into Argonaute-associated small RNA fragments that can function as microRNAs.

• MethylFlash 5-mC RNA Methylation ELISA Easy Kit (Fluorometric)
  For fluorescence-based quantitation of global 5-mC RNA methylation in an ELISA-like format


• EpiNext 5-mC RNA Bisulfite-Seq Easy Kit (Illumina)
  For RNA bisulfite conversion, followed by a "post-bisulfite" library preparation process


• Methylamp RNA Bisulfite Conversion Kit
  For robust, chemical-based bisulfite treatment of RNA for methylated cytosine analysis

Identify m6A modifications by enriching for parts of a genome where small amounts of m6A are present. Then prepare these segments for next generation sequencing by constructing a library. Our unique method, called CUT&RUN RNA m6A enrichment (cleavage under target and recover using nuclease for RNA m6A enrichment), isolates only RNA segments at the antibody binding locations and simultaneously captures and cleaves just under the target. This streamlined procedure takes RNA to library cDNA in just hours.

• EpiQuik CUT&RUN m6A RNA Enrichment Kit
  MeRIP based on CUT&RUN for increased specificity and signals


• EpiNext CUT&RUN RNA m6A-Seq Kit
  All-in-one MeRIP and NGS library preparation based on CUT&RUN