Expand upon your understanding of RNA methylation with the help of EpiGentek’s complete suite of RNA methylation products. From directly quantifying the "fifth RNA base" N6-Methyladenosine (m6A) to measuring global 5-mC RNA and more, our kits make it easy for you to determine how RNA methylation influences your research project.
m6A RNA Methylation
Investigate N6-methyladenosine (m6A) -- the most common and abundant methylation modification in RNA molecules present in eukaryotes. Known as the "fifth RNA base", m6A RNA modifications are reversible, highly dynamic, and likely play important biological roles comparable to DNA methylation and histone modification.
Study the occurrence of 5-methylcytosine (5-mC) in RNA -- a well-known methylation modification found in various RNA molecules including tRNAs, rRNAs, mRNAs and non-coding RNAs (ncRNAs). Evidence has shown that RNA cytosine methylation affects various biological processes, such as RNA stability and mRNA translation. The loss of 5-mC in vault RNAs may cause aberrant processing into Argonaute-associated small RNA fragments that can function as microRNAs.
Identify m6A modifications by enriching for parts of a genome where small amounts of m6A are present. Then prepare these segments for next generation sequencing by constructing a library. Our unique method, called CUT&RUN RNA m6A enrichment (cleavage under target and recover using nuclease for RNA m6A enrichment), isolates only RNA segments at the antibody binding locations and simultaneously captures and cleaves just under the target. This streamlined procedure takes RNA to library cDNA in just hours.