EpiQuik Nuclear Extraction Kit II (Nucleic Acid-Free)

The EpiQuik™ Nuclear Extraction Kit II (Nucleic Acid-Free) is a complete set of optimized reagents to provide a simple and selective method for isolating nuclear proteins free of nucleic acids attached, to be used in a variety of applications in just 60 minutes. These applications may include Western blot, protein-DNA binding assays, nuclear enzyme assays, and any other procedures requiring optimized nucleic acid-free nuclear proteins but not enzymatic activity. The protocol is fast and easy to use and also isolates very abundant yields of nuclear extract from mammalian cells or tissue samples. Some of the kit's highlighted features include the following:

• Pre-optimized and simple step-wise 1-hour protocol.
• Conveniently includes all essential reagents to carry out a nucleic acid-free nuclear extraction.
• Standardized procedure for reproducible results.

Principle & Procedure
The EpiQuik™ Nuclear Extraction Kit II (Nucleic Acid-Free) simply applies our proprietary nuclear protein isolation buffers to cells/tissues. After pre-lysis and lysis buffers are treated, the nucleic acid-free nuclear proteins are easily extracted for immediate use or storage under proper conditions.

Note: This item was formerly catalog no. OP-0002-2 (OP00022, OP-00022, OP0002-2)