The EpiQuik™ General Protein-DNA Binding Assay Kit (Fluorometric) is a convenient package of tools that allows the experimenter to investigate protein-DNA interaction in vitro efficiently. The entire procedure can be completed within 3 hours and produces far more superior results than any competitor kits. The EpiQuik™ General Protein-DNA Binding Assay Kits are suitable for analyzing protein-DNA interactions in vitro, specifically for detecting transcription factor activation using mammalian tissue and cell extracts. The kit has the following advantages:
Principle & ProcedureThe EpiQuik™ General Protein-DNA Binding Assay Kit (Fluorometric) is designed for measuring the transcription factor (TF) of DNA binding activity in nuclear extracts. In an assay using this kit, target TF in nuclear extract is captured by high affinity antibodies onto the assay microwells. A double strand oligonucleotide (oligo) containing DNA binding consensus sequence for the target TF is added into the microwell and bound to active TF in the binding assay buffer. The bound oligo is then fluorometrically detected.