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EpiQuik DNA Methyltransferase (DNMT) Activity/Inhibition Assay Kit (Fluorometric)

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Schematic procedure for the EpiQuik DNA Methyltransferase (DNMT) Activity/Inhibition Assay Kit (Fluorometric).
Input Type: DNA
Research Area: DNA Methylation
Target Application: Activity Measurement
Vessel Format: 96-Well Plate
100% Guarantee: 6 months
Catalog No.SizePriceQty
P-3004-4848 assays $328.00 
P-3004-9696 assays $544.00 
Availability: Usually Ships In 1-2 Days 
Product Overview





We recommend using a newer version of this product: EpiQuik DNMT Activity/Inhibition Assay Ultra Kit (Fluorometric)




The EpiQuik™ DNA Methyltransferase Activity/Inhibition Assay Kit (Fluorometric) is a complete set of tools to fluorometrically measure Dnmt activity and inhibition at very fast speeds. The kit is suitable for measuring Dnmt activity/inhibition from a broad range of species including mammalian cells/tissues, plants, and bacteria.

WHY CHOOSE THE EPIQUIK™ DNA METHYLTRANSFERASE ACTIVITY/INHIBITION ASSAY KIT (FLUOROMETRIC),

  • Extremely fast procedure, which can be completed within 3 hours.
  • Innovative fluorometric assay with high sensitivity for detecting Dnmt activity generated from as low as 2 ng of enzyme.
  • Strip microplate format makes the assay flexible: manual or high throughput analysis.
  • Simple, reliable, and consistent assay conditions.
Product Details

The EpiQuik™ DNA Methyltransferase Activity/Inhibition Assay Kit (Fluorometric) is designed for measuring total Dnmt activity (de novo, maintenance). In an assay with this kit, the unique cytosine-rich DNA substrate is stably coated on the strip wells. These wells are specifically treated to have a high DNA absorption ability. Dnmt enzymes transfer a methyl group to cytosine from Adomet to methylate the DNA substrate. The methylated DNA can be recognized with an anti-5-methylcytosine antibody. The ratio or amount of methylated DNA, which is proportional to enzyme activity, can then be fluorometrically quantified through an ELISA-like reaction.

SCHEMATIC PROCEDURE:  
 
Product Components

MD1 (10X Wash Buffer)
MD2 (Dnmt Assay Buffer)
MD3 (Adomet, 8 mM)*
MD4 (Dnmt Positive Control)*
MD5 (Capture Antibody)*
MD6 (Detection Antibody, 200 µg/ml)*
MD7 (Fluoro Developer)*
MD8 (Fluoro Enhancer)*
MD9 (Fluoro Dilution)
8-Well Substrate-Coated Strip (with Frame)
User Guide

* For maximum recovery of the products, centrifuge the original vial after thawing prior to opening the cap.

User Guide & MSDS

[User Guide]*
*Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing info@epigentek.com along with your contact information and institution name.

[Material Safety Data Sheet]
Replace Your Santa Cruz Abs
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