The EpiQuik™ Dnmt1 Assay Kit is a convenient package of tools that allows the experimenter to measure the amount of Dnmt1 at tremendously fast speeds and consistency; superior and safer than all other current methods. The kit is ready-to-use and provides all the essential components needed to carry out a successful Dnmt1 assay experiment without the need for electrophoresis and transfer process. The EpiQuik™ Dnmt1 Assay Kit is suitable for measuring Dnmt amounts quantitatively from fresh tissue and cultured cells of human and mouse.

WHY CHOOSE THE EPIQUIK™ DNMT1 ASSAY KIT?

• The fastest procedure, which can be finished within 3 hours.
• Innovative colorimetric assay to quantitatively measure Dnmt1 amount without the need for electrophoresis.
• Strip microplate format makes the assay flexible: manual or high throughput analysis.
• Simple, reliable, and consistent assay conditions.

The EpiQuik™ Dnmt1 Assay Kit is designed for measuring total Dnmt1 amount from tissues or cells. In an assay with this kit, the unique Dnmt affinity substrate is stably coated on the strip well. The sample is added into the well and Dnmt1 contained in the sample binds to the substrate. The bound Dnmt1 can be recognized with a specific Dnmt1 antibody and colorimetrically quantified through an ELISA-like reaction. The amount of Dnmt1 is proportional to the intensity of color development.

SCHEMATIC PROCEDURE:
	Nuclear extracts were prepared from MCF-7 cells using the EpiQuik Nuclear Extraction Kit and total amount of Dnmt was measured.

D1 (10X Wash Buffer)
D2 (Assay Buffer)
D3 (Dnmt1 Standard, 20 µg/ml)*
D4 (Affinity Antibody, 500 µg/ml)*
D5 (Detection Antibody, 200 µg/ml)*
D6 (Developing Solution)
D7 (Stop Solution)
8-Well Assay Strips (with Frame)
User Guide

* For maximum recovery of the products, centrifuge the original vial after thawing prior to opening the cap.

[User Guide]* *Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing info@epigentek.com along with your contact information and institution name.

[Material Safety Data Sheet]

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Hervouet, E. et. al. (November 2008). Tumor induction by disruption of the Dnmt1, PCNA and UHRF1 interactions. Nature Precedings. Full PDF Article

Roll, J. D. et. al. (January 2008). DNMT3b overexpression contributes to a hypermethylator phenotype in human breast cancer cell lines. Molecular Cancer. 7: 15. Full PDF Article