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EpiQuik Dnmt1 Assay Kit

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For measuring the amount of DNMT1 protein in samples

Citations (21) | Write a Review
Suggested Workflow
Nuclear Protein Extraction
 
 
DNMT Assay
 
Schematic procedure for using the EpiQuik Dnmt1 Assay Kit.
Nuclear extracts were prepared from MCF-7 cells using the EpiQuik Nuclear Extraction Kit and total amount of Dnmt was measured.
Input Type: Nuclear Extracts, Purified Enzyme
Research Area: DNA Methylation
Target Application: Amount Quantitation
Vessel Format: 96-Well Plate
100% Guarantee: 6 months
Catalog No.SizePriceQty
P-3011-248 assays $399.00 
P-3011-396 assays $549.00 
Availability: Usually Ships In 1-2 Days 
Product Overview

The EpiQuik™ Dnmt1 Assay Kit is a convenient package of tools that allows the experimenter to measure the amount of Dnmt1 at tremendously fast speeds and consistency; superior and safer than all other current methods. The kit is ready-to-use and provides all the essential components needed to carry out a successful Dnmt1 assay experiment without the need for electrophoresis and transfer process. The EpiQuik™ Dnmt1 Assay Kit is suitable for measuring Dnmt amounts quantitatively from fresh tissue and cultured cells of human and mouse. The kit has the following advantages:

  • The fastest procedure, which can be finished within 3 hours.
  • Innovative colorimetric assay to quantitatively measure Dnmt1 amount without the need for electrophoresis.
  • Strip microplate format makes the assay flexible: manual or high throughput analysis.
  • Simple, reliable, and consistent assay conditions.

Principle & Procedure
The EpiQuik™ Dnmt1 Assay Kit is designed for measuring total Dnmt1 amount from tissues or cells. In an assay with this kit, the unique Dnmt affinity substrate is stably coated on the strip well. The sample is added into the well and Dnmt1 contained in the sample binds to the substrate. The bound Dnmt1 can be recognized with a specific Dnmt1 antibody and colorimetrically quantified through an ELISA-like reaction. The amount of Dnmt1 is proportional to the intensity of color development.

 
Fig. 1. Schematic procedure for using the EpiQuik Dnmt1 Assay Kit.

Fig. 2. Nuclear extracts were prepared from MCF-7 cells using the EpiQuik Nuclear Extraction Kit and total amount of Dnmt was measured.

Product Components

D1 (10X Wash Buffer)
D2 (Assay Buffer)
D3 (Dnmt1 Standard, 20 µg/ml)*
D4 (Affinity Antibody, 500 µg/ml)*
D5 (Detection Antibody, 200 µg/ml)*
D6 (Developing Solution)
D7 (Stop Solution)
BB (Blocking Buffer)
8-Well Assay Strips (with Frame)
User Guide

* For maximum recovery of the products, centrifuge the original vial after thawing prior to opening the cap.

User Guide & MSDS

[User Guide]*
*Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing info@epigentek.com along with your contact information and institution name.

[Material Safety Data Sheet]
Product Citations

Rajpathak SN et. al. (February 2017). Micro RNAs and DNA methylation are regulatory players in human cells with altered X chromosome to autosome balance. Sci Rep. 7:43235.

Cechinel LR et. al. (October 2016). Treadmill exercise induces age and protocol-dependent epigenetic changes in prefrontal cortex of Wistar rats. Behav Brain Res. 313:82-7.

Wu F et. al. (March 2016). Oral administration of Schisandra chinensis extract suppresses Dnmt1 expression in Kunming mice ovaries Genes Genom. :1-8.

Mytych J et. al. (February 2016). Prolonged Effects of Silver Nanoparticles on p53/p21 Pathway-Mediated Proliferation, DNA Damage Response, and Methylation Parameters in HT22 Hippocampal Neuronal Cells. Mol Neurobiol.

Mukhopadhyay P et. al. (October 2015). Cigarette smoke induces proteasomal-mediated degradation of DNA methyltransferases and methyl CpG-/CpG domain-binding proteins in embryonic orofacial cells. Reprod Toxicol.

Yang G et. al. (July 2015). DNA methyltransferase 3, a target of microRNA-29c, contributes to neuronal proliferation by regulating the expression of brain-derived neurotrophic factor. Mol Med Rep. 12(1):1435-42.

Selokar NL et. al. (April 2015). Downregulation of DNA Methyltransferase 1 in Zona-Free Cloned Buffalo (Bubalus bubalis) Embryos by Small Interefering RNA Improves In Vitro Development But Does Not Alter DNA Methylation Level. Cell Reprogram. 17(2):89-94.

Das DS et. al. (December 2014). Anti-Myeloma Activity of a Novel Free Radical Inducer Rrx-001 Blood. 124(21):4712-4712.

Chu M et. al. (April 2014). Hypermethylation-mediated transcriptional repression of TMPRSS2 in androgen receptor-negative prostate cancer cells. Exp Biol Med (Maywood).

Bielak-Zmijewska A et. al. (February 2014). A comparison of replicative senescence and doxorubicin-induced premature senescence of vascular smooth muscle cells isolated from human aorta. Biogerontology. 15(1):47-64.

Nettersheim D et. al. (December 2013). Analysis of TET expression/activity and 5mC oxidation during normal and malignant germ cell development. PLoS One. 8(12):e82881.

Wu Z et. al. (September 2013). Ultraviolet B enhances DNA hypomethylation of CD4+ T cells in systemic lupus erythematosus via inhibiting DNMT1 catalytic activity. J Dermatol Sci. 71(3):167-73.

Jang JY et. al. (February 2013). ANT2 suppression by shRNA may be able to exert anticancer effects in HCC further by restoring SOCS1 expression. Int J Oncol. 42(2):574-82.

Zhao J et. al. (January 2013). Genome-wide identification of Epstein-Barr virus-driven promoter methylation profiles of human genes in gastric cancer cells. Cancer. 119(2):304-12.

Desplats P et. al. (March 2011). Alpha-synuclein sequesters Dnmt1 from the nucleus: a novel mechanism for epigenetic alterations in Lewy body diseases. J Biol Chem. 286(11):9031-7.

Jang JY et. al. (September 2010). Short-hairpin RNA-induced suppression of adenine nucleotide translocase-2 in breast cancer cells restores their susceptibility to TRAIL-induced apoptosis by activating JNK and modulating TRAIL receptor expression. Mol Cancer. 9:262.

Hervouet E et. al. (June 2010). Disruption of Dnmt1/PCNA/UHRF1 interactions promotes tumorigenesis from human and mice glial cells. PLoS One. 5(6):e11333.

Liu Z et. al. (May 2009). Modulation of DNA methylation by a sesquiterpene lactone parthenolide. J Pharmacol Exp Ther. 329(2):505-14.

Majid S et. al. (April 2009). BTG3 tumor suppressor gene promoter demethylation, histone modification and cell cycle arrest by genistein in renal cancer. Carcinogenesis. 30(4):662-70.

Roll JD et. al. (January 2008). DNMT3b overexpression contributes to a hypermethylator phenotype in human breast cancer cell lines. Mol Cancer. 7:15.

J. D. Roll et. al. (January 2008). DNMT3b overexpression contributes to a hypermethylator phenotype in human breast cancer cell lines Molecular Cancer. 7(15) Full PDF Article

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