Epigenetic Research Papers 8/14/23 - 8/18/23

Padalino G et. al. (March 2023). Chemical modulation of Schistosoma mansoni lysine specific demethylase 1 (SmLSD1) induces wide-scale biological and epigenomic changes Wellcome Open Res. 8:146.
The article investigates the effects of inhibiting Schistosoma mansoni lysine specific demethylase 1 (SmLSD1) with a collection of small molecules. Compound 33 is identified as a potent inhibitor, impacting various life cycle stages of the parasite and leading to biological and epigenomic changes, suggesting its potential as a candidate for the development of next-generation anti-schistosomal drugs.
Products Used: EpiQuik Total Histone Extraction Kit

Cuesta-Borràs E et. al. (August 2023). DPPA3-HIF1α axis controls colorectal cancer chemoresistance by imposing a slow cell-cycle phenotype Cell Rep. 42(8):112927.
The article investigates chemoresistance in colorectal cancer (CRC) and identifies DPPA3 as a key player in inducing a slow-cycling phenotype that promotes resistance to chemotherapy. The study uncovers a feedback loop between DPPA3 and HIF1α that suppresses FOXM1 expression, resulting in a delayed cell-cycle progression, and highlights DPPA3 as a potential biomarker for chemotherapeutic resistance in CRC patients.
Products Used: DNMT1 Polyclonal Antibody

Koch J et. al. (August 2023). Reinvestigating the clinical relevance of the m6A writer METTL3 in urothelial carcinoma of the bladder iScience. 26(8):107300.
The article focuses on the role of METTL3, a major writer of m6A RNA modification, in urothelial carcinoma of the bladder (UCB). The study demonstrates that while METTL3 knockout reduces the oncogenic phenotype and m6A levels of UCB cell lines, its complete depletion is hindered by alternative isoforms, and the clinical relevance of METTL3 in UCB is complex, suggesting it might serve as a diagnostic biomarker but its therapeutic potential needs careful evaluation.
Products Used: CRISPR/Cas9 Monoclonal Antibody [7A9]

Zhang K et. al. (July 2023). Cloak Scavenges the Reactive Oxygen Species around the Larvae of Drino inconspicuoides (Diptera: Tachinidae) Insects. 14(7)
The study investigates the function of a newly discovered structure called the "cloak" in the development of the parasitoid insect Drino inconspicuoides within its host. The cloak, formed outside the funnel structure, is shown to have high activities of antioxidant enzymes, which suggests that it scavenges reactive oxygen species generated during melanization of the funnel, thus protecting the developing parasitoid larva from oxidative damage.
Products Used: EpiQuik Superoxide Dismutase Activity/Inhibition Assay Kit (Colorimetric)

Popov M et. al. (June 2023). Effect of Arsenic Soil Contamination on Stress Response Metabolites, 5-Methylcytosine Level and CDC25 Expression in Spinach Toxics. 11(7)
The study investigated the impact of arsenic soil contamination on spinach plants and found that arsenic accumulation in plants was higher in roots than in leaves, indicating a limited translocation ability. Arsenic contamination led to changes in stress response metabolites, DNA methylation (5-methylcytosine levels), and the expression of the CDC25 gene in roots and leaves of spinach, highlighting the complex effects of arsenic on plant physiology.
Products Used: MethylFlash Methylated DNA Quantification Kit (Fluorometric)

Shi Y et. al. (August 2023). CHLORIDE CHANNEL-b mediates vacuolar nitrate efflux to improve low nitrogen adaptation in Arabidopsis Plant Physiol.
The study focused on the role of CHLORIDE CHANNEL-b (CLC-b) in vacuolar nitrate efflux and its impact on Arabidopsis plants under low-nitrogen conditions. The research revealed that CLC-b expression increased during nitrate starvation, and mutants with altered CLC-b expression exhibited changes in various physiological parameters, highlighting its role in improving plant adaptation to low-nitrogen environments.
Products Used: EpiQuik Plant ChIP Kit

Hou X et. al. (August 2023).  METTL14 reverses liver fibrosis by inhibiting NOVA2 through an m6A-YTHDF2-dependent mechanism Hepatol Commun. 7(8)
The study explored the role of METTL14-induced N6-methyladenosine (m6A) modification in liver fibrosis (LF) and its impact on hepatic stellate cells (HSCs). The research revealed that decreased METTL14 levels in LF led to reduced m6A modification and increased HSC activation, exacerbating fibrosis, and demonstrated that METTL14 inhibited NOVA2 through an m6A-YTHDF2-dependent mechanism, contributing to LF progression, while NOVA2 exhibited a protective effect by partially reversing the fibrotic damage.
Products Used: EpiQuik m6A RNA Methylation Quantification Kit (Colorimetric)

Huang T et. al. (December 2023). Comprehensive analysis of m6A methylomes in idiopathic pulmonary arterial hypertension Epigenetics. 18(1):2242225.
The article investigates the role of m6A RNA methylation in idiopathic pulmonary arterial hypertension (IPAH). The study comprehensively analyzes m6A methylomes in the peripheral blood of IPAH patients, revealing altered m6A levels of specific genes such as RPS27A, SMAD3, TP53, and FoxO3, and implicating m6A-related regulators like METTL3 and YTHDF1 in the pathogenesis of IPAH.
Products Used: EpiQuik m6A RNA Methylation Quantification Kit (Colorimetric)

Ni WJ et. al. (August 2023). Genetic and pharmacological inhibition of METTL3 alleviates renal fibrosis by reducing EVL m6A modification through an IGF2BP2-dependent mechanism  Clin Transl Med. 13(8):e1359.
The study investigates the role of N6-methyladenosine (m6A) modification in renal fibrosis and its regulation by METTL3 and IGF2BP2. It is found that METTL3 enhances m6A modification of EVL mRNA, leading to increased EVL expression through an IGF2BP2-dependent mechanism, which contributes to renal fibrosis progression. Additionally, the TCM monomer isoforsythiaside is identified as a potential anti-renal fibrosis agent by inhibiting METTL3 activity and the METTL3/EVL m6A axis.
Products Used: EpiQuik m6A RNA Methylation Quantification Kit (Colorimetric)

Wang C et. al. (August 2023). EGR1 mediates METTL3/m6A/CHI3L1 to promote osteoclastogenesis in osteoporosis Genomics. :110696.
The study investigates the EGR1-mediated METTL3/m6A/CHI3L1 axis in osteoporosis. It demonstrates that EGR1 promotes METTL3 transcription, leading to increased m6A-modified CHI3L1 levels, ultimately promoting osteoclast differentiation and osteoporosis development.
Products Used: EpiQuik m6A RNA Methylation Quantification Kit (Colorimetric)

Zhao X et. al. (July 2023).  Alterations of the m6A Methylation Induced by TGF-β2 in ARPE-19 Cells Front Biosci (Landmark Ed). 28(7):148.
The article investigates the impact of TGF-β2 on m6A methylation in ARPE-19 cells. Through RNA-seq and MeRIP-seq analyses, the study identifies altered gene expression and m6A methylation patterns, particularly related to EMT processes, suggesting a significant role of m6A in TGF-β2-induced EMT in retinal pigmental epithelial cells.
Products Used: EpiQuik m6A RNA Methylation Quantification Kit (Colorimetric)

Wang X et. al. (August 2023). Therapeutic targeting of FUBP3 phase separation by GATA2-AS1 inhibits malate-aspartate shuttle and neuroblastoma progression via modulating SUZ12 activity Oncogene.
The article investigates how GATA2-AS1 affects the malate-aspartate shuttle (MAS) and progression of neuroblastoma. The study reveals that GATA2-AS1 inhibits FUBP3 phase separation, leading to modulation of SUZ12 activity, which in turn suppresses MAS and aggressive behavior in neuroblastoma, suggesting a potential therapeutic target for this childhood malignancy.
Products Used: EpiQuik CUT&RUN m6A RNA Enrichment (MeRIP) Kit

Gu Z et. al. (July 2023). WTAP-mediated m6A modification of IFNE is required for antiviral defense in condyloma acuminata J Dermatol Sci.
The study explores the role of IFN-ε in keratinocytes during HPV infection and condyloma acuminata (CA) progression. The research identifies that IFN-ε is upregulated in CA warts and inhibits HPV infection susceptibility, and m6A modification of IFNE mRNA is mediated by WTAP, highlighting the importance of IFN-ε in innate immunity against HPV and its potential for regulating immune responses to treat genital warts.
Products Used: EpiQuik CUT&RUN m6A RNA Enrichment (MeRIP) Kit