Kim J et. al. (April 2023). Trans- and Multigenerational Effects of Isothiazolinone Biocide CMIT/MIT on Genotoxicity and Epigenotoxicity in Daphnia magna. Toxics. 11(4)
The study evaluated the toxicity of CMIT/MIT on Daphnia magna, a type of plankton, and its trans- and multigenerational effects over four consecutive generations. The results showed harmful effects on various endpoints, with different response patterns according to different exposure histories, emphasizing the importance of considering exposure duration and multigenerational observations in evaluating ecotoxicity and risk management.
(Products Used: MethylFlash Global DNA Methylation (5-mC) ELISA Easy Kit (Colorimetric))
Liu X et. al. (April 2023). CsRVE1 promotes seasonal greening of albino Camellia sinensis cv. Huangkui by activating chlorophyll biosynthesis. Tree Physiol.
In this study, researchers investigated the regulatory mechanisms of seasonal greening in albino tea plants, which is a crucial survival strategy during which dysfunctional chloroplasts recover and chlorophyll biosynthesis increases in albino leaves. The researchers found that a nuclear-located Myb-like transcription factor called CsRVE1 can positively modulate seasonal greening in albino Camellia sinensis cv. Huangkui leaves by activating the expression of genes involved in light harvesting and chlorophyll biosynthesis.
(Products Used: EpiQuik Plant ChIP Kit)
Meharwade T et. al. (April 2023). Cross-activation of FGF, NODAL, and WNT pathways constrains BMP-signaling-mediated induction of the totipotent state in mouse embryonic stem cells Cell Rep. 42(5):112438.
The study explored the signaling mechanisms regulating the totipotent state and coexistence of different states in cultured mouse embryonic stem cells (ESCs). The findings revealed that bone morphogenetic protein (BMP) signaling induces the totipotent state but is constrained by the cross-activation of FGF, NODAL, and WNT pathways, and rational inhibition of these pathways enhances the proportion of totipotent cells.
(Products Used: MuERVL-Gag Polyclonal Antibody)
Zhang L et. al. (May 2023). MBD2 facilitates tumor metastasis by mitigating DDB2 expression Cell Death Dis. 14(5):303.
The study showed that the expression of the methyl-CpG-binding domain 2 (MBD2) is highly correlated with tumor metastasis in patients with LUAD. Knockdown of MBD2 repressed DDB2 expression, attenuated epithelial-mesenchymal transition (EMT), and suppressed tumor metastasis in different types of tumor cells.
(Products Used: MethylFlash Methylated DNA Quantification Kit (Fluorometric))
Shi ZD et. al. (May 2023). Integrative multi-omics analysis depicts the methylome and hydroxymethylome in recurrent bladder cancers and identifies biomarkers for predicting PD-L1 expression Biomark Res. 11(1):47.
The study used a multi-omics approach to analyze the genome, transcriptome, methylome, and hydroxymethylome landscape of urinary bladder cancer (UBC) patients and identified biomarkers for predicting PD-L1 expression. The researchers found that epigenetic alterations are more involved in PD-L1 regulation and UBC recurrence than genetic mutations and that 5mC and 5hmC levels should not be combined as clinical biomarkers.
(Products Used: BisulPlus Loci 5mC & 5hmC Detection PCR Kit)
Zhou X et. al. (May 2023). YWHAB knockdown inhibits cell proliferation whilst promoting cell cycle arrest and apoptosis in colon cancer cells through PIK3R2. Exp Ther Med. 25(5):193.
The study aimed to determine the role of YWHAB in colon cancer and its mechanism of action. The results showed that YWHAB depletion inhibited cell proliferation, promoted cell cycle arrest and apoptosis in colon cancer cells by targeting PIK3R2 and activating the PI3K/AKT signaling pathway.
(Products Used: YWHAB Polyclonal Antibody)
Stoccoro A et. al. (March 2023). Prenatal Environmental Stressors and DNA Methylation Levels in Placenta and Peripheral Tissues of Mothers and Neonates Evaluated by Applying Artificial Neural Networks Genes (Basel). 14(4)
The study examined the effects of prenatal exposure to environmental stressors on DNA methylation levels in placental and peripheral tissues of mothers and neonates, using artificial neural networks. The results suggest that exposure to environmental stressors during pregnancy can lead to aberrant methylation levels in genes linked to embryogenesis in both placenta and peripheral tissues of mothers and infants, potentially providing peripheral biomarkers of environmental exposure.
(Products Used: MethylFlash Methylated DNA 5-mC Quantification Kit (Colorimetric), MethylFlash Hydroxymethylated DNA 5-hmC Quantification Kit (Colorimetric))
Li Z et. al. (April 2023). N 6-methyladenosine modification contributes to respiratory syncytial virus infection Heliyon. 9(4):e15307.
The study explored the relationship between N 6-methyladenosine (m6A) modification and respiratory syncytial virus (RSV) infection. The researchers evaluated the changes in m6A modification during RSV infection and found that it played a significant role in the infection process, with implications for the development of new therapeutic strategies.
(Products Used: EpiQuik m6A RNA Methylation Quantification Kit (Colorimetric))
Zhong D et. al. (April 2023). Identification of Metabolism-Related Proteins as Biomarkers of Insulin Resistance and Potential Mechanisms of m6A Modification Gene. :147430.
The study aimed to identify metabolism-related proteins that could be used as potential biomarkers of insulin resistance (IR) and investigate the role of m6A modification in the pathogenesis of IR. The study identified four potential biomarkers, including GCK, FBP1, FGR, and FASN, and found that the expression of FASN was positively correlated with the methylation levels of FASN in adipose tissue samples collected from healthy individuals and patients with IR.
(Products Used: EpiQuik CUT&RUN m6A RNA Enrichment (MeRIP) Kit)
DeRosa H et. al. (May 2023). Maternal immune activation alters placental histone-3 lysine-9 tri-methylation, offspring sensorimotor processing, and hypothalamic transposable element expression in a sex-specific manner Neurobiol Stress. 24:100538.
The article explores the effects of maternal immune activation (MIA) on epigenetic modifications and transposable elements in Sprague-Dawley rats. The study found that MIA altered the chromatin landscape of the placenta and was associated with long-term sensorimotor processing deficits and increased transposable element expression in a sex-specific manner.
(Products Used: EpiQuik Total Histone Extraction Kit)
Cai Y et. al. (April 2023). Alteration of m6A-Tagged RNA Profiles in Bone Originated from Periprosthetic Joint Infection J Clin Med. 12(8)
The study investigated the N6-methyladenine (m6A) modification in periprosthetic joint infection (PJI) and found that there were significant differences in overall m6A levels and expression of m6A-related genes between PJI and aseptic failure patients. The study also identified differential m6A-modified mRNAs involved in infection and immune response, bone metabolism, and programmed cell death, suggesting that m6A modification could be a therapeutic target for developing effective treatment strategies.
(Products Used: EpiQuik m6A RNA Methylation Quantification Kit (Colorimetric))
Wang B et. al. (July 2023). Gestational 1-nitropyrene exposure causes anxiety-like behavior partially by altering hippocampal epigenetic reprogramming of synaptic plasticity in male adult offspring J Hazard Mater. 453:131427.
This study shows that gestational exposure to 1-Nitropyrene (1-NP) causes anxiety-like behavior in male adult offspring by altering the epigenetic reprogramming of synaptic plasticity in the hippocampus. The findings suggest that α-ketoglutarate (α-KG) supplementation can prevent hippocampal synaptic damage and improve anxiety-like behavior in male adult offspring.
(Products Used: Epigenase 5mC-Hydroxylase TET Activity/Inhibition Assay Kit (Colorimetric))