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CTBP1 Polyclonal Antibody

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Western blot analysis of extracts of mouse brain, using CTBP1 Antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded human liver cancer using CTBP1 antibody at dilution of 1:100 (40x lens).
Application: IHC, WB
Areas of Research: Transcription Factors
Clonality: Polyclonal
Conjugate: Unconjugated
Host: Rabbit
Isotype: IgG
Purification: Affinity Purified
Reactivity: Human, Mouse
100% Guarantee: 6 months
Catalog No.SizePriceQty
A-2705-05050 µl $189.00 
A-2705-100100 µl $299.00 
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Product Overview

Background
CtBP1 (C-terminal binding protein 1) was first recognized as a cellular factor that interacts with the C-terminal portion of adenovirus E1A, a protein involved in the transcriptional regulation of key cellular genes. CtBP1 is able to regulate gene activity through its intrinsic dehydrogenase activity and by interacting with Polycomb Group (PcG) proteins during development. Along with its homologue, CtBP2, it acts as a transcriptional corepressor of zinc-finger homeodomain factor deltaEF1 to regulate a wide range of cellular processes through transrepression mechanisms. Through its direct interaction with PRDM16, CtBP1 has been shown to be involved in brown adipose tissue differentiation by mediating the repression of white fat genes and directing differentiation toward the brown fat gene program. CtBP1 also plays a role in lipid metabolic pathways and membrane fission by regulating the fission machinery operating Golgi tubular networks. CtBP1 has recently been shown to repress transcription of BRCA1 via a redox regulated mechanism. Furthermore, it is thought that downregulation of BRCA1 and E-cadherin in invasive ductal breast carcinoma correlates directly with activation of CtBP1.

Description
CTBP1 Polyclonal Antibody. Unconjugated. Raised in: Rabbit.

Formulation
Liquid. PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Specificity
Human, Mouse

Isotype
IgG

Uniprot ID
Q13363

Purification
Affinity Purified

Immunogen
Recombinant Protein of Human CTBP1

Storage
Shipped at 4°C. Store at -20°C. Avoid multiple freeze/thaw cycles.

Alternative Names
BARS, MGC104684

Application
WB, IHC; Recommended dilution: WB 1:500 - 1:2000 IHC 1:50 - 1:200

User Guide & MSDS

[User Guide]*
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Customer Reviews

Rating by m*****@ucdenver.edu Verified Purchase Reviewed on: Tuesday 20 December, 2016
Application Description
The product I used is EpiQuick Chromatin Immunoprecipitation (ChiP) Kit (P-2002), and CtBP1 antibody (A-2705). I used this for a Chip assay to examine genes that bind with CtBP1 protein. The antibody works good with human cells on CHiP assay.
Rating by c*******@mail.nih.gov Verified Purchase Reviewed on: Thursday 27 August, 2015
Application Description
Western Blot, only worked when primary antibody was incubated with blot overnight. Did not work after 1-hour incubation with primary antibody.

Procedural Details
1. Blocked membrane with 5% milk solution for 1-hour
2. Diluted antibody 1:500 in 5% milk solution.
3. Incubated membrane with CTBP1 antibody diluted 1:500 in 5% milk overnight
4. Washed with 1X TBE with Tween 4x for 5 minutes each
5. Incubated membrane with Secondary antibody against primary antibody for 1-hour
6. Washed with 1X TBE with Tween 4x for 5 minutes each
7. Visualized bands with detection reagent
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