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CBX5 Polyclonal Antibody

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Western blot analysis of extracts of various cell lines, using CBX5 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Immunofluorescence analysis of HeLa cells using CBX5 antibody. Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200ug extracts of HeLa cells using 1ug CBX5 antibody. Western blot was performed from the immunoprecipitate using CBX5 antibody at a dilition of 1:1000.
Application: ChIP, IF, IP, WB
Areas of Research: Chromatin Remodeling
Clonality: Polyclonal
Conjugate: Unconjugated
Host: Rabbit
Isotype: IgG
Purification: Affinity Purified
Reactivity: Human, Mouse, Rat
100% Guarantee: 6 months
Catalog No.SizePriceQty
A-2701-05050 µl $189.00 
A-2701-100100 µl $299.00 
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Product Overview

Background
Heterochromatin protein 1 (HP1) is a family of heterochromatic adaptor molecules involved in both gene silencing and higher order chromatin structure. All three HP1 family members (alpha, beta, and gamma) are primarily associated with centromeric heterochromatin; however, HP1beta and gamma also localize to euchromatic sites in the genome. HP1 proteins are approximately 25 kDa in size and contain a conserved amino-terminal chromodomain, followed by a variable hinge region and a conserved carboxy-terminal chromoshadow domain. The chromodomain facilitates binding to histone H3 tri-methylated at Lys9, a histone "mark" closely associated with centromeric heterochromatin. The variable hinge region binds both RNA and DNA in a sequence-independent manner. The chromoshadow domain mediates the dimerization of HP1 proteins, in addition to binding multiple proteins implicated in gene silencing and heterochromatin formation, including the SUV39H histone methyltransferase, the DNMT1 and DNMT3a DNA methyltransferases, and the p150 subunit of chromatin-assembly factor-1 (CAF1). In addition to contributing to heterochromatin formation and propagation, HP1 and SUV39H are also found complexed with retinoblastoma (Rb) and E2F6 proteins, both of which function to repress euchromatic gene transcription in quiescent cells. HP1 proteins are subject to multiple types of post-translational modifications, including phosphorylation, acetylation, methylation, ubiquitination, and sumoylation, suggesting multiple means of regulation.

Description
CBX5 Polyclonal Antibody. Unconjugated. Raised in: Rabbit.

Formulation
Liquid. PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Specificity
Human, Mouse, Rat

Isotype
IgG

Uniprot ID
P45973

Purification
Affinity Purified

Immunogen
Recombinant Protein of Human CBX5

Storage
Shipped at 4°C. Store at -20°C. Avoid multiple freeze/thaw cycles.

Alternative Names
CBX5, HP1, HP1A

Application
WB, IF, IP, ChIP; Recommended dilution: WB 1:500 - 1:2000, IF 1:50 - 1:200, IP 1:50 - 1:200, ChIP 1:20 - 1:100

User Guide & MSDS

[User Guide]*
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