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PHD3 Monoclonal Antibody new product

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Western blot analysis of various lysates using PHD3 mAb at 1:20000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Western blot analysis of lysates from BxPC-3 cells, using PHD3 mAb at 1:20000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using PHD3 mAb at dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0. 01M Citrate buffer (pH 6. 0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human esophageal cancer using PHD3 mAb at dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0. 01M Citrate buffer (pH 6. 0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human placenta using PHD3 mAb at dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0. 01M Citrate buffer (pH 6. 0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse spleen using PHD3 mAb at dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0. 01M Citrate buffer (pH 6. 0) prior to IHC staining.
Confocal imaging of U-2 OS cells using PHD3 mAb (dilution 1:200)(Red). The cells were counterstained with a-Tubulin Mouse mAb (dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 60x.
Application: ELISA, IF/ICC, IHC-P, WB
Clonality: Monoclonal Antibodies
Conjugate: Unconjugated
Host: Rabbit
Isotype: IgG
Purification: Affinity Purified
Reactivity: Human, Mouse
100% Guarantee: 6 months
Catalog No.SizePriceQty
A78540-100100 µl $308.00 
Order now & get it by Tuesday, June 9th  
Product Overview

Background
Enables peptidyl-proline 4-dioxygenase activity. Involved in several processes, including activation of cysteine-type endopeptidase activity involved in apoptotic process; peptidyl-proline hydroxylation to 4-hydroxy-L-proline; and response to hypoxia. Located in cytosol and nucleus. Implicated in renal cell carcinoma. Biomarker of clear cell renal cell carcinoma.

Description
PHD3 Monoclonal Antibody. Unconjugated. Raised in: Rabbit.

Formulation
Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.

Specificity
Human, Mouse

Isotype
IgG

Uniprot ID
Q9H6Z9

Purification
Affinity Purified

Immunogen
Synthetic peptide. Please contact EpigenTek for more information.

Storage
Store at -20°C. Avoid freeze / thaw cycles.

Alternative Names
PHD3; HIFPH3; HIFP4H3

Application
WB, IHC-P, IF/ICC, ELISA; Recommended dilution: WB, 1:10000 - 40000; IHC-P, 1:100 - 1:1000; IF/ICC, 1:50 - 1:200; ELISA, Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.

Datasheet & SDS

PDF Datasheet/User Guide: A78540.pdf
If the above file is not available online, you can request a copy by contacting us.
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