BackgroundThis gene encodes a tumor suppressor protein and is expressed in a wide variety of tissue types. p53 is involved in regulating cell growth, replication, and apoptosis. It binds to Mdm2, SV40 T antigen, and the human Papilloma virus E6 protein. This gene can detect DNA damage and facilitates its repair. Mutations of p53 are associated with a wide variety of malignant tumors, including breast, ovary, bladder, colon, lung, and melanoma.
DescriptionMouse monoclonal antibody to p53, Clone PAB122
FormulationLiquid. In PBS containing 50% glycerol and 0.09% sodium azide.
ImmunogenSV40 transformed 3T3 cells.
SpecificityHuman, Mouse, Rat
IsotypeIgG2b
PurificationProtein G- affinity purified.
StorageStore at -20°C. Avoid multiple freeze/thaw cycles.
Alternative NamesTRP53, Tumor protein p53
ApplicationFC, ICC, IHC, IP, WB (1:250 colorimetric, 1:250 ECL)
Fig. 1. Western blot analysis of p53: Lane 1: MW marker, Lane 2: A431, Lane 3: rat brain, Lane 4: mouse brain.