Epigentek Home
Cart (0)
Datasheet
Track
Account
     »    »    »   
  Quick Order      Quote Lookup

NDUFB8 Monoclonal Antibody new product

Write a Review
▲
▼
Western blot analysis of various lysates using NDUFB8 mAb at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Western blot analysis of various lysates using NDUFB8 mAb at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry analysis of paraffin-embedded Human breast cancer using NDUFB8 mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0. 01M Citrate buffer (pH 6. 0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human colon using NDUFB8 mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0. 01M Citrate buffer (pH 6. 0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human kidney using NDUFB8 mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0. 01M Citrate buffer (pH 6. 0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human liver cancer using NDUFB8 mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0. 01M Citrate buffer (pH 6. 0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human tonsil using NDUFB8 mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0. 01M Citrate buffer (pH 6. 0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Rat kidney using NDUFB8 mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0. 01M Citrate buffer (pH 6. 0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Rat liver using NDUFB8 mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0. 01M Citrate buffer (pH 6. 0) prior to IHC staining.
Confocal imaging of HeLa cells using NDUFB8 mAb (dilution 1:200) followed by a further incubation with Cy3-conjugated Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). The cells were counterstained with TOMM20 Mouse mAb (dilution 1:1000) followed by incubation with conjugated Goat Anti-Mouse IgG (H+L) Ab (dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
Application: ELISA, IF-P, IF/ICC, IHC-P, WB
Clonality: Monoclonal Antibodies
Conjugate: Unconjugated
Host: Rabbit
Isotype: IgG
Purification: Affinity Purified
Reactivity: Human, Mouse, Rat
100% Guarantee: 6 months
Catalog No.SizePriceQty
A77975-100100 µl $308.00 
Order now & get it by Tuesday, June 9th  
Product Overview

Background
Involved in mitochondrial respiratory chain complex I assembly. Located in endoplasmic reticulum and mitochondrion. Part of mitochondrial respiratory chain complex I. Implicated in nuclear type mitochondrial complex I deficiency 32. Biomarker of Alzheimer's disease and Parkinson's disease.

Description
NDUFB8 Monoclonal Antibody. Unconjugated. Raised in: Rabbit.

Formulation
Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.

Specificity
Human, Mouse, Rat

Isotype
IgG

Uniprot ID
O95169

Purification
Affinity Purified

Immunogen
Recombinant protein (or fragment). Please contact EpigenTek for more information.

Storage
Store at -20°C. Avoid freeze / thaw cycles.

Alternative Names
ASHI; CI-ASHI; MC1DN32; NDUFB8

Application
WB, IF/ICC, IF-P, IHC-P, ELISA; Recommended dilution: WB, 1:1000 - 1:6000; IF/ICC, 1:200 - 1:400; IF-P, 1:200 - 1:2000; IHC-P, 1:200 - 1:2000; ELISA, Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.

Datasheet & SDS

PDF Datasheet/User Guide: A77975.pdf
If the above file is not available online, you can request a copy by contacting us.
Product FAQ  

No FAQs found at this time

Company
About Epigentek
Press Releases
Events Calendar
Corporate Sustainability
Career Opportunities
What Is Epigenetics?
Contact Us
 Ordering
Ordering Info & Return Policy
Distributors & Resellers

Support
Technical Support
Download Datasheets
Resource Bulletins
 Promotions & Offers
Current Promotions
New Lab Startup Program
Risk-Free Testing: Pioneer Program
Rewards Program
Product Reviews
Application Guarantee
Sign Up For Exclusive Offers
Request a Quote
 Stay Informed
Terms & Conditions | Privacy Policy | Site Map
Copyright © EpigenTek Group Inc. All rights reserved.
Terms & Conditions | Privacy Policy Copyright © 2020 EpiGentek