This human brain genomic DNA is highly purified and carefully isolated from human adult normal brain tissues. Evidence shows that DNA isolated from human brain tissues contains high amounts of 5-hydroxymethylcytosine (5-hmC), a modified cytosine which plays an important role in epigenetic regulation of gene function. The average lengthy of the DNA is greater than 50 kb and is suitable for genomic library construction and as a control DNA for 5-hmC detection. A260/280 is between 1.8 and 2.0, and A260/230 is about 2.0 as tested by spectrophotometer.
10 μg in 50 μl
10 mM Tris-HCl pH 8.0, 1 mM EDTA
Purified free of contaminating protein and RNA. A260/280 ratio: 1.8, and A260/230 ratio:~2.0
Human brain genomic DNA should be stored at -20°C for up to 12 months. Repeated freeze/thaw cycles should be avoided.
Human adult normal brain tissue
For research use only.
Genomic library construction; Control for 5-hmC detection