ELISA-like Histone Methylation or Acetylation Measurement
Post-translational modifications (PTMs) of histones such as methylation and acetylation can influence chromatin remodeling and transcription regulation. Thus, evaluating histone modifications could help researchers understand more about gene activation/repression and overall cell health.
One simple way to determine specific histone methylation or acetylation status is through the use of a quantification assay. EpiGentek offers a complete series of ELISA-like assay kits, in both colorimetric and fluorimetric, that measure specific histone H3 & H4 methylation or acetylation levels in their samples.
About Histone Methylation
Histone methylation involves the addition of methyl groups to histone proteins, causing either transcription activation or repression of a gene depending on the amino acid affected. Methylation can occur at various locations on histone tails; however, the majority appear at lysine (K) and arginine residues. Some of the most widely studied sites include H3K4, H3K9, H3K27, H4K20, H3K36, H3K79, H4K20, H3R8, and H4R3.
About Histone Acetylation Histone acetylation involves the addition of an acetyl group to the lysine residues on the tail of a histone, regulating chromatin structure and mobilizing transcription machinery to gene promoters. Histone acetylation plays an important role in controlling many cellular processes, such as cellular proliferation and apoptosis. Some of the most widely studied acetylation sites include
H3K9, H3K14, H3K18, H3K27, H3K36, H3K56, H4K5, H4K8, H4K12, H4K16, and H4K16.
Histone H3 and H4 Methylation Quantification Kits Include: (*Fluorometric Version)