Save $100 on Antibodies Save $100 when you buy $500 in antibodies. Use code E1705ABR. Expires June 30th. » Learn More
Epigentek Home
 
Epigentek Free Shipping

EpiQuik Global Pan-Methyl Histone H3K4 Quantification Kit (Colorimetric)

Share:

For ELISA-like measurement of total H3K4me1me2me3 amounts

Citations (4) | Write a Review
Suggested Workflow
Histone Protein Extraction
 
 
Histone H3 Methylation Quantification
 
Schematic procedure for using the EpiQuik Global Pan-Methyl Histone H3K4 Quantification Kit (Colorimetric).
Input Type: Histone Extracts
Research Area: Histone Methylation
Target Application: Amount Quantitation
Vessel Format: 96-Well Plate
100% Guarantee: 6 months
Catalog No.SizePriceQty
P-3028-9696 assays $493.00 
Availability: Usually Ships In 1-2 Days 
Product Overview

The EpiQuik™ Global Pan-Methyl Histone H3-K4 Quantification Kit (Colorimetric) is a convenient package of tools that allows the experimenter to specifically measure global mono-, di-, and tri-methylation of histone H3-K4 colorimetrically using a variety of mammalian cells (human, mouse, etc.) including fresh and frozen tissues, cultured adherent and suspension cells. The kit has the following advantages:

  • Quick and efficient procedure, which can be finished within 2.5 hours.
  • Innovative colorimetic assay without the need for radioactivity, electrophoresis, and chromatography.
  • Simultaneous quantification of mono-, di-, and tri-methylated H3-K4 with the detection limit as low as 2 ng/well and detection range from 20 ng-5 µg/well of histone extracts.
  • The control is conveniently included for the quantification of the amount of mono-, di-, and tri-methylated H3-K4.
  • Strip microplate format makes the assay flexible: manual or high throughput.
  • Simple, reliable, and consistent assay conditions.

Background Information
Epigenetic activation or inactivation of genes play a critical role in many important human diseases, especially in cancer. A major mechanism for epigenetic inactivation of the genes is methylation of CpG islands in genome DNA caused by DNA methyltransferases. Histone methyltransferases (HMTs) control or regulate DNA methylation through chromatin-dependent transcription repression or activation. HMTs transfer 1-3 methyl groups from S-adenosyl-Lmethionine to the lysine and arginine residues of histone proteins. SET1, SET7/9, Ash1, ALL-1, MLL, ALR, Trx, and SMYD3 are histone methyltransferases that catalyze methylation of histone H3 at lysine 4 (H3-K4) in mammalian cells. H3-K4 mono-methylation is associated with silenced euchromatin regions in the genome and may serve as a global epigenetic mark and function in gene repression. In contrast, di- and tri-methylation is associated with transcriptionally active euchromatin regions in the genome, and may serve as a global epigenetic mark of highly transcribed genes. Increased global H3-K4 methylation is also found to be involved in some pathological processes such as cancer progression. The patterns of global H3-K4 methylation can also change by inhibition or activation of HMTs. Thus, quantitative detection of the patterns of global histone H3-K4 methylation would provide useful information for better understanding epigenetic regulation of gene activation, and for developing HMT-targeted drugs. The EpiQuik™ Global Pan-Methyl Histone H3-K4 Quantification Kit (Colorimetric) provides a tool for measuring global mono-, di-, and tri-methylation of histone H3-K4.

Principle & Procedure
This kit is designed for simultaneously measuring global histone H3-K4 mono-, di-, and tri-methylation. In an assay with this kit, the methylated histone H3 at lysine 4 is captured to the strip wells coated with antibodies specifically for mono-, di-, and tri-methyl H3-K4. The captured mono-, di-. and tri-methylated histone H3-K4 can then be detected with a labeled detection antibody, followed by a color development reagent. The ratio of mono-, di-, and tri-methylated H3-K4 is proportional to the intensity of absorbance. The absolute amount of the methylated H3-K4 can be quantitated by comparing to the standard control.

Starting Materials
Input material should be purified histone extracts. In general, the input amount should be from 50 ng to 200 ng per well of histone extracts.

 

Product Components

 

C1 (10X Wash Buffer)
C2 (Antibody Buffer)
C3 (Detection Antibody, 1 mg/ml)*
C4 (Color Developer)
C5 (Stop Solution)
Standard Control (100 µg/ml)*
8-Well Sample Strips (with Frame)
8-Well Standard Control Strips
User Guide 

* For maximum recovery of the products, centrifuge the original vial prior to opening the cap.

 

User Guide & MSDS

[User Guide]*
*Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing info@epigentek.com along with your contact information and institution name.

Product Citations

Liu Y et. al. (February 2016). Effects of Wutou Decoction on DNA Methylation and Histone Modifications in Rats with Collagen-Induced Arthritis Evid. Based Complement. Alternat. Med.. 2016:1-9.

Morishita M et. al. (December 2014). In vitro histone lysine methylation by NSD1, NSD2/MMSET/WHSC1, and NSD3/WHSC1L. BMC Struct Biol. 14(1):25.

Monteiro JB et. al. (March 2014). Endometriosis is characterized by a distinct pattern of histone 3 and histone 4 lysine modifications. Reprod Sci. 21(3):305-18.

Ponomarev I et. al. (February 2012). Gene coexpression networks in human brain identify epigenetic modifications in alcohol dependence. J Neurosci. 32(5):1884-97.

Replace Your Santa Cruz Abs
Related Products

Researchers Also Purchased
EpiQuik Total Histone Extraction Kit
EpiQuik Total Histone Extraction Kit
EpiQuik Global Pan-Methyl Histone H3K9 Quantification Kit (Colorimetric)
EpiQuik Global Pan-Methyl Histone H3K9 Quantification Kit (Colorimetric)
EpiQuik Global Pan-Methyl Histone H3K27 Quantification Kit (Colorimetric)
EpiQuik Global Pan-Methyl Histone H3K27 Quantification Kit (Colorimetric)
EpiQuik Total Histone H3 Quantification Kit (Colorimetric)
EpiQuik Total Histone H3 Quantification Kit (Colorimetric)
Epigentek is an epigenetics company that specializes in epigenetic kits, antibodies, reagents, and services for epigenetic research in DNA methylation, histone modification, and chromatin studies. Terms & Conditions | Privacy Policy | Site Map
Copyright © 2017 Epigentek Group Inc. All rights reserved.