Accurately measure global 5-mC or 5-hmC in just two hours
Measuring global 5-mC and 5-hmC levels in DNA doesn’t need to be a big hassle. Forget those cumbersome techniques that can be lengthy, costly and often produce too much unnecessary data. Instead, save yourself the extra effort and quantify global DNA methylation levels in one easy step with our MethylFlash™ Global DNA Methylation and Hydroxymethylation ELISA Easy Kits.
So fast...finish in just 2 hours
ELISA-like format enables direct quantitation of genomic DNA methylation in a “one-step” reaction
Significantly more affordable than costly LC-mass spectrometry
Schematic procedure for both the MethylFlash™ Global DNA Methylation (5-mC) and Hydroxymethylation (5-hmC) ELISA Easy Kits.
EpigenTek vs. Competitors: Performance Comparison
Feature
EpigenTek
Competitor Z
Competitor C
Protocol Time
2 hours
>3 hours
>3 hours
Sample Input Type
Isolated DNA is directly used for the assay; no blocking needed
Isolated DNA requires single strand conversion; blocking needed
Urine; Isolated DNA (requires single strand conversion, nuclease digestion, and alkaline phosphatase treatment)
Input Amount
100 ng optimal
100 ng optimal
2 µg of DNA minimum per assay
Sensitivity
LOD 0.05%
LOD >0.5%
150 nM of 5-mC deoxynucleoside
Controls
Synthesized PC (methylated) and NC (unmethylated) oligos
PC and NC E. coli gDNA (require single strand conversion); PC methylated with CpG Methylase
Fast: Reduced steps so that the entire procedure only needs 2 hours
Robust: Improved kit composition allows the assay to have a greater "signal window" with reduced variation between replicates
Accurate: Optimized positive controls that can be fractionalized in percentage scale, allowing the assay to be more accurate and highly comparable with HPLC-MS analysis.
Fast: Reduced steps so that the entire procedure only needs 2 hours
Robust: Improved kit composition allows the assay to have a greater "signal window" with reduced variation between replicates
Accurate: Optimized positive controls that can be fractionalized in percentage scale, allowing the assay to be more accurate and highly comparable with HPLC-MS analysis.
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Measuring global 5-mC and 5-hmC levels in DNA doesn’t need to be a big hassle. Forget those cumbersome techniques that can be lengthy, costly and often produce too much unnecessary data. Instead, save yourself the extra effort and quantify global DNA methylation levels in one easy step with our 
