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Introduction to Epigenetics
DNMT Activity/Inhibition ELISA Easy Kit

Super Sensitive DNA Methyltransferase Activity/Inhibition Measurement

EpiGentek’s EpiQuik DNMT Activity/Inhibition ELISA Easy Kit is a complete set of optimized buffers and reagents that allows the experimenter to measure DNA methyltransferase activity or inhibition using nuclear extracts or purified enzymes from a broad range of species in a fast and easy manner. This kit is a further refinement of its predecessor kit by simplifying the steps, significantly decreasing assay time, and increasing assay sensitivity.

  • Fast - Reduced steps so that the entire procedure only needs 2 hours and 10 minutes.
  • Sensitive - Detection limit can be as low as 0.2 ng of purified enzymes, which is ten times better than the predecessor kit.
  • Convenient - Ready to use with all essential components. No need for radioactivity or any special equipment.
  • Robust - Improved kit composition allows the assay to have a greater “signal window” with reduced variation between replicates.
  • Flexible - 96 strip-well microplate format makes the assay available for manual or high throughput analysis.

 

Demonstration of high sensitivity and specificity of DNMT activity/inhibition achieved by using nuclear extracts with the EpiQuik™ DNMT Activity/Inhibition ELISA Easy Kit (Colorimetric). Nuclear extracts were prepared from Hela cell line.

Demonstration of high sensitivity and specificity of DNMT activity/inhibition achieved by using nuclear extracts with the EpiQuik™ DNMT Activity/Inhibition ELISA Easy Kit (Colorimetric).



Schematic procedure of the EpiQuik DNMT Activity/Inhibition ELISA Easy Kit (Colorimetric).
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