Super Sensitive DNA Methyltransferase Activity/Inhibition Measurement
EpiGentek’s EpiQuik DNMT Activity/Inhibition ELISA Easy Kit is a complete set of optimized buffers and reagents that allows the experimenter to measure DNA methyltransferase activity or inhibition using nuclear extracts or purified enzymes from a broad range of species in a fast and easy manner. This kit is a further refinement of its predecessor kit by simplifying the steps, significantly decreasing assay time, and increasing assay sensitivity.
Fast - Reduced steps so that the entire procedure only needs 2 hours and 10 minutes.
Sensitive - Detection limit can be as low as 0.2 ng of purified enzymes, which is ten times better than the predecessor kit.
Convenient - Ready to use with all essential components. No need for radioactivity or any special equipment.
Robust - Improved kit composition allows the assay to have a greater “signal window” with reduced variation between replicates.
Flexible - 96 strip-well microplate format makes the assay available for manual or high throughput analysis.
Demonstration of high sensitivity and specificity of DNMT activity/inhibition achieved by using nuclear extracts with the EpiQuik™ DNMT Activity/Inhibition ELISA Easy Kit (Colorimetric). Nuclear extracts were prepared from Hela cell line.
Demonstration of high sensitivity and specificity of DNMT activity/inhibition achieved by using nuclear extracts with the EpiQuik™ DNMT Activity/Inhibition ELISA Easy Kit (Colorimetric).