Extraction

DNMT & DNA Demethylase Kits

Accurately assess DNA methyltransferase & demethylase activity levels

DNMT Screen for DNA methyltransferase (DNMT) or demethylase in a few simple steps. Let EpigenTek's easy and convenient ELISA-like assays do the leg work. Each of our kits provides everything needed to measure DNMT or DNA demethylase activity or inhibition levels from nuclear extracts or purified enzymes.

Measure DNMT Activity
EpigenTek’s EpiQuik DNMT Activity/Inhibition ELISA Easy Kit is a complete set of optimized buffers and reagents that allows the experimenter to measure DNA methyltransferase activity or inhibition using nuclear extracts or purified enzymes from a broad range of species in a fast and easy manner. This kit further refined its predecessor kit by simplifying the steps, significantly decreasing assay time and increasing assay sensitivity. (Fluorometric version also available, # P-3010).

Measure Demethylase Activity
EpigenTek’s EpiQuik DNA Demethylase Activity/Inhibition Assay Ultra Kit is a complete set of optimized buffers and reagents that allows the experimenter to measure DNA demethylase activity or inhibition using nuclear extracts from a broad range of species in a fast and easy manner.

Measure TET & TDG Enzyme Activity
EpigenTek’s Epigenase TET & TDG Activity/Inhibition Assay Kits each come with a complete set of optimized buffers and reagents that allows the experimenter to directly measure TET hydroxylase or thymine DNA Glycosylase (TDG) activity or inhibition using nuclear extracts or purified enzymes from a broad range of species in a fast and easy manner.

Featured Kits

Click image to see more details	EpiQuik DNMT Activity/Inhibition ELISA Easy Kit For measuring DNA methyltransferase activity or inhibition from nuclear extracts or purified enzymes Fast: Reduced steps so that the entire procedure only needs 2 hours and 10 minutes Super Sensitive: Detection limit can be as low as 0.2 ng of purified enzymes, which is ten times better than the predecessor kit Convenient: Ready to use with all essential components without the need for radioactivity or any special equipment Robust: Improved kit composition allows the assay to have a greater “signal window” with reduced variation between replicates Flexible: 96 strip-well microplate format makes the assay available for manual or high throughput analysis Catalog #: P-3139
Click image to see more details	EpiQuik DNA Demethylase Activity/Inhibition Assay Ultra Kit For measuring total DNA demethylase activity or inhibition from nuclear extracts Fast: Easy-to-follow steps that can be finished within 4 hours Sensitive: Detection limit can be as low as 1 µg of nuclear extract Convenient: Ready to use with all essential components without the need for radioactivity or any special equipment Flexible: 96 strip-well microplate format makes the assay available for manual or high throughput analysis Catalog #: P-3008
Click image to see more details	Epigenase 5mC-Hydroxylase TET Activity/Inhibition Assay Kits For measuring TET enzymatic activity or inhibition levels from nuclear extracts or purified TET isoforms Fast: Easy-to-follow steps that can be finished in only 5 hours Sensitive: Detection limit can be as low as 20 ng of purified TET1 hydroxylase Convenient: Ready to use with all essential components without the need for special equipment Flexible: 96 strip-well microplate format makes the assay available for manual or high throughput analysis Catalog #: P-3086 & P-3087
Click image to see more details	Epigenase Thymine DNA Glycosylase (TDG) Activity/Inhibition Assay Kit For measuring TDG enzyme activity or inhibition from nuclear extracts or purified TDG enzymes Fast: Easy-to-follow steps allow the entire procedure can be finished within 5 hours Sensitive: Activity can be detected from as low as 20 ng of purified TDG protein Convenient: Ready to use with all essential components without the need for DNA cleavage, electrophoresis, or chromatography Flexible: 96 strip-well microplate format makes the assay available for manual or high throughput analysis. Catalog #: P-3094