This is part of our DNA Methylation Workflow, click the image steps below for more details.

View side-by-side comparison of DNA Methylation Quantification kits.

Global DNA Methylation Kits

Accurately measure global 5-mC or 5-hmC in just two hours

Measuring global 5-mC and 5-hmC levels in DNA doesn’t need to be a big hassle. Forget those cumbersome techniques that can be lengthy, costly and often produce too much unnecessary data. Instead, save yourself the extra effort and quantify global DNA methylation levels in one easy step with our MethylFlash™ Global DNA Methylation and Hydroxymethylation ELISA Easy Kits.

	So fast...finish in just 2 hours
	ELISA-like format enables direct quantitation of genomic DNA methylation in a “one-step” reaction
	Significantly more affordable than costly LC-mass spectrometry

Schematic procedure for both the MethylFlash™ Global DNA Methylation (5-mC) and Hydroxymethylation (5-hmC) ELISA Easy Kits.

How are these new kits different from our previous MethylFlash kits?

The MethylFlash Easy kits, while still retaining the many advantageous features of the previous kits, utilizes a “one-step” ELISA reaction to eliminate the need for multiple sample and antibody binding steps. The protocols of these new kits are simplified, reducing the procedure times by 50% versus the previous MethylFlash kits. Some adjustments were made to also reduce the CV% between wells. The greater “signal window” of each kit, along with adjusted calculation formulas, makes these new kits very accurate. In a nutshell, MethylFlash Easy kits are faster, easier, more robust, and more accurate.

Kit Details
Click image to see more details	MethylFlash Global DNA Methylation (5-mC) ELISA Easy Kit (Colorimetric) For quantitation of global DNA methylation via a one-step ELISA method Fast: Reduced steps so that the entire procedure only needs 2 hours Robust: Improved kit composition allows the assay to have a greater "signal window" with reduced variation between replicates Accurate: Optimized positive controls that can be fractionalized in percentage scale, allowing the assay to be more accurate and highly comparable with HPLC-MS analysis. Catalog #: P-1030
Click image to see more details	MethylFlash Global DNA Hydroxymethylation (5-hmC) ELISA Easy Kit (Colorimetric) For quantitation of global DNA methylation via a one-step ELISA method Fast: Reduced steps so that the entire procedure only needs 2 hours Robust: Improved kit composition allows the assay to have a greater "signal window" with reduced variation between replicates Accurate: Optimized positive controls that can be fractionalized in percentage scale, allowing the assay to be more accurate and highly comparable with HPLC-MS analysis. Catalog #: P-1032