Accurately measure global 5-mC or 5-hmC in just two hours
Measuring global 5-mC and 5-hmC levels in DNA doesn’t need to be a big hassle. Forget those cumbersome techniques that can be lengthy, costly and often produce too much unnecessary data. Instead, save yourself the extra effort and quantify global DNA methylation levels in one easy step with our MethylFlash™ Global DNA Methylation and Hydroxymethylation ELISA Easy Kits.
So fast...finish in just 2 hours
ELISA-like format enables direct quantitation of genomic DNA methylation in a “one-step” reaction
Significantly more affordable than costly LC-mass spectrometry
Schematic procedure for both the MethylFlash™ Global DNA Methylation (5-mC) and Hydroxymethylation (5-hmC) ELISA Easy Kits.
How are these new kits different from our previous MethylFlash kits?
The MethylFlash Easy kits, while still retaining the many advantageous features of the previous kits, utilizes a “one-step” ELISA reaction to eliminate the need for multiple sample and antibody binding steps. The protocols of these new kits are simplified, reducing the procedure times by 50% versus the previous MethylFlash kits. Some adjustments were made to also reduce the CV% between wells. The greater “signal window” of each kit, along with adjusted calculation formulas, makes these new kits very accurate. In a nutshell, MethylFlash Easy kits are faster, easier, more robust, and more accurate.
Fast: Reduced steps so that the entire procedure only needs 2 hours
Robust: Improved kit composition allows the assay to have a greater "signal window" with reduced variation between replicates
Accurate: Optimized positive controls that can be fractionalized in percentage scale, allowing the assay to be more accurate and highly comparable with HPLC-MS analysis.
Fast: Reduced steps so that the entire procedure only needs 2 hours
Robust: Improved kit composition allows the assay to have a greater "signal window" with reduced variation between replicates
Accurate: Optimized positive controls that can be fractionalized in percentage scale, allowing the assay to be more accurate and highly comparable with HPLC-MS analysis.
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Measuring global 5-mC and 5-hmC levels in DNA doesn’t need to be a big hassle. Forget those cumbersome techniques that can be lengthy, costly and often produce too much unnecessary data. Instead, save yourself the extra effort and quantify global DNA methylation levels in one easy step with our 
