Site-Specific & Genome-Wide DNA Methylation Assays
▶DNA Bisulfite Conversion
Get reliable DNA methylation data with EpigenTek’s easy-to-use bisulfite conversion kits. The BisulFlash DNA Modification Kit delivers pure, bisulfite-modified DNA in just 30 minutes. For simultaneous 5mC and 5hmC detection by PCR, try our unique BisulPlus Loci 5mC & 5hmC Detection PCR Kit. Our proprietary formula protects against DNA loss, ensuring high recovery efficiency for next-gen sequencing and pyrosequencing.
▶Methylated DNA Immunoprecipitation
If you’re seeking a quick, efficient way to identify cytosine modifications in DNA, EpigenTek’s MeDIP and hMeDIP kits are ideal. Our kits use high-affinity antibodies specific to 5mC or 5hmC, ensuring accurate enrichment of methylated or hydroxymethylated DNA in a convenient 96-well format. The captured DNA is ready for PCR, microarray, or sequencing analysis.
▶Methylated DNA Amplification
Discover the advantages of Methylated DNA Amplification with our advanced techniques, including Methylation-specific PCR (MSP) using the Methylamp MS-qPCR Fast Kit and EpiQuik Quantitative PCR Fast Kit. These powerful tools offer critical insights into gene regulation and the influence of methylation on health and disease, helping you drive your research forward.
▶Next Generation Sequencing
Transform your DNA analysis with Next-Generation Sequencing (NGS) solutions from EpigenTek. Our affordable options include comprehensive kits designed to construct high-quality DNA libraries from low quantities or challenging samples. For example, the EpiNext™ RRBS Library Fast Kit enables rapid preparation of reduced representation bisulfite sequencing (RRBS) DNA libraries, ensuring high coverage from minimal starting material.
DNA Bisulfite Conversion
Featured Kits
Click image to see more details
BisulFlash DNA Modification Kit
For lightning-fast bisulfite treatment of DNA for methylation-specific PCR analysis in just 30 minutes
Fast: The entire procedure is as short as 30 minutes without any reagent setup time
Highly Efficient: Completely convert unmethylated cytosine into uracil: modified DNA > 99.9%
DNA Protection: Protect against DNA degradation of which more than 90% of DNA loss can be prevented, allowing for greater recovery
High Sensitivity: Start with the lowest amount of input DNA for modification -- only 0.2 ng or just 50 cells
Simultaneous Reading: DNA treatment is simultaneously prepared for both 5hmC and 5mC, allowing for parallel identification in a loci- or gene-specific manner by qPCR
Fast: The enitire procedure from DNA bisulfite treatment to PCR products can be completed within 5 hours
Highly Efficient: Completely converts C into uracil (>99%) and 5mC to thymine (>95%)
High Specificity: 5hmC is discriminated from C and 5mC as well as from other modified cytosines 5fC and 5caCr
Flexible: Perform 24 reactions for 5hmC and 24 reactions for 5mC analysis simultaneously, or perform 48 reactions for just 5mC
Highly Efficient: Enrichment ratio of positive/negative control > 100
Convenient: Contains all reagents required to enrich and capture methylated DNA fragments and compatible various downstream analysis workflows including hMeDIP-PCR and hMeDIP-chip
Flexible: Strip-well microplate format makes the assay flexible: manual or high throughput
Catalog #: P-1038
Methylated DNA Amplification
Click image to see more details
Methylamp MS-qPCR Fast Kit
Perform fast, specific, sensitive and reproducible methylation-specific quantitative PCR
Fast: Can be finished within 70 minutes
Highly Efficient: Abundant yields due to high amplification efficiency
Convenient: Master mix format allows easy reaction setup
Flexible: Can be used with any block-based real-time PCR instrument
Cart (0)
