Efficiently generate bisulfite-converted DNA for methylation analysis
Yield reliable and accurate information about DNA methylation states with EpiGentek’s fast and easy to use DNA bisulfite conversion kits. In as little as 30 minutes, you can go from input to pure bisulfite modified DNA when using the BisulFlash DNA Modification Kit. Or, detect both 5mC and 5hmC simultaneously by PCR with our unique BisulPlus Loci 5mC & 5hmC Detection PCR Kit. Enhanced with a proprietary composition that protects against DNA loss, our kits preserve important DNA recovery efficiency and are suitable for next generation methylation sequencing and pyrosequencing.
Schematic procedure for the BisulFlash DNA Modification Kit (Cat# P-1026).
About DNA Bisulfite Conversion
It is well demonstrated that DNA methylation plays an important role in the regulation of gene expression, tumorigenesis, and other genetic and epigenetic diseases. Thus, detecting methylation in some genes of diseased cells could provide very useful information for discrimination of that disease. Bisulfite modification of genomic DNA, followed by PCR amplification, cloning, and sequencing of individual PCR amplimers, is a critical method to yield reliable and accurate information on DNA methylation states. By treating DNA with bisulfite, cytosine residues are converted to uracil while leaving 5-methylcytosine intact. For a complete cytosine conversion, single-stranded DNA is necessary.
Kit Details
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BisulFlash DNA Modification Kit
For lightning-fast bisulfite treatment of DNA for methylation-specific PCR analysis in just 30 minutes
Fast: The entire procedure is as short as 30 minutes without any reagent setup time
Highly Efficient: Completely convert unmethylated cytosine into uracil: modified DNA > 99.9%
DNA Protection: Protect against DNA degradation of which more than 90% of DNA loss can be prevented, allowing for greater recovery
High Sensitivity: Start with the lowest amount of input DNA for modification -- only 0.2 ng or just 50 cells
Simultaneous Reading: DNA treatment is simultaneously prepared for both 5hmC and 5mC, allowing for parallel identification in a loci- or gene-specific manner by qPCR
Fast: The enitire procedure from DNA bisulfite treatment to PCR products can be completed within 5 hours
Highly Efficient: Completely converts C into uracil (>99%) and 5mC to thymine (>95%)
High Specificity: 5hmC is discriminated from C and 5mC as well as from other modified cytosines 5fC and 5caCr
Flexible: Perform 24 reactions for 5hmC and 24 reactions for 5mC analysis simultaneously, or perform 48 reactions for just 5mC
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Yield reliable and accurate information about DNA methylation states with EpiGentek’s fast and easy to use DNA bisulfite conversion kits. In as little as 30 minutes, you can go from input to pure bisulfite modified DNA when using the 
