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Guan Y et. al. (September 2024). The effect of nitrosative stress on histone H3 and H4 acetylation in Phytophthora infestans life cycle Plant Physiol Biochem. 216:109129.
This study investigates how nitrosative stress affects histone acetylation in the phytopathogen Phytophthora infestans. They found that reactive nitrogen species (RNS) increased acetylation of histones H3 and H4, particularly in sporulating hyphae during potato colonization. This was linked to the up-regulation of acetyltransferases PifHAC3 and PifHAM1, which target specific acetylation marks. Enhanced histone acetylation was associated with increased expression of pathogenicity-related genes. The findings suggest that RNS-driven transcriptional reprogramming via histone acetylation contributes to the adaptability of P. infestans in response to environmental stresses.
Products Used: EpiQuik Total Histone H3 Acetylation Detection Fast Kit (Colorimetric), EpiQuik Total Histone H4 Acetylation Detection Fast Kit (Colorimetric)
Caballero L et. al. (September 2024). Connecting high-resolution 3D chromatin maps with cell division and cell differentiation at the root apical meristem Plant Cell Rep. 43(10):232.
This study employed marker-free technologies to examine chromatin dynamics at the root apical meristem in trichoblast and atrichoblast cells. Researchers created three-dimensional chromatin maps and analyzed histone modifications (H3K4me1 and H3K9me2), revealing asymmetric chromatin distribution and structural differences during mitosis. The findings suggest that these chromatin characteristics are linked to cell cycle and differentiation processes, with indications of coordinated cell division and nuclear migration in epidermal and cortical tissues, potentially regulated by an unknown mechanism.
Products Used: EpiQuik Total Histone Extraction Kit, EpiQuik Histone H3 Modification Multiplex Assay Kit (Colorimetric)
Shang J et. al. (September 2024). Genome-wide DNA methylation sequencing reveals the involvement of ferroptosis in hepatotoxicity induced by dietary exposure to food-grade titanium dioxide Part Fibre Toxicol. 21(1):37.
This study examined the effects of dietary titanium dioxide (E 171) on liver health in mice, focusing on epigenetic changes. Mice exposed to E 171 for 28 or 84 days showed that long-term exposure reduced DNA methylation and caused liver inflammation and damage. Whole-genome sequencing revealed significant methylation alterations linked to ferroptosis, an iron-dependent cell death mechanism. The findings suggest that E 171 induces hepatotoxicity through epigenetic changes that activate ferroptosis, highlighting potential risks of this food additive.
Products Used: MethylFlash Global DNA Methylation (5-mC) ELISA Easy Kit (Colorimetric), MethylFlash Global DNA Hydroxymethylation (5-hmC) ELISA Easy Kit (Colorimetric)
Xu X et. al. (September 2024). hucMSC-Ex alleviates inflammatory bowel disease in mice by enhancing M2-type macrophage polarization via the METTL3-Slc37a2-YTHDF1 axis Cell Biol Toxicol. 40(1):74.
This study explored the effects of human umbilical cord mesenchymal stem cell-derived exosomes (hucMSC-Ex) on inflammatory bowel disease (IBD) in mice, focusing on macrophage polarization. hucMSC-Ex promoted M2 macrophage polarization and regulated m6A levels by enhancing the expression of METTL3 and YTHDF1. Slc37a2 was identified as a target, with hucMSC-Ex increasing the binding of METTL3 to Slc37a2 mRNA and its interaction with YTHDF1, reducing macrophage inflammation. These results suggest hucMSC-Ex as a potential therapeutic strategy for IBD through m6A modulation.
Products Used: EpiQuik m6A RNA Methylation Quantification Kit (Colorimetric), EpiQuik CUT&RUN m6A RNA Enrichment (MeRIP) Kit
Zhang Y et. al. (September 2024). The WOX9-WUS modules are indispensable for the maintenance of stem cell homeostasis in Arabidopsis thaliana Plant J.
This study investigated the WOX9-WUS modules' roles in maintaining stem cell homeostasis in Arabidopsis thaliana. Researchers found that amiR-WOX9 transgenic plants exhibited premature shoot apical meristem (SAM) termination and altered flower development, similar to the wus-101 mutant. WUS expression decreased in amiR-WOX9 plants but increased in UBQ10::WOX9-GFP plants without changing meristem size. WOX9 was shown to directly bind to the WUS promoter, activating its expression, while WUS also represses its own expression. The interaction between WOX9 and WUS negatively affects WUS levels. Overall, WOX9 is crucial for regulating WUS and maintaining SAM stability.
Products Used: EpiQuik Plant ChIP Kit
Fan Y et. al. (September 2024). Tet1-mediated activation of the Ampk signaling by Trpv1 DNA hydroxymethylation exerts neuroprotective effects in a rat model of Parkinson's disease Funct Integr Genomics. 24(5):161.
This study examined TET1-mediated hydroxymethylation of TRPV1 and its neuroprotective role in a rat model of Parkinson's disease (PD). TET1 binds to the TRPV1 promoter, regulating its expression and influencing oxidative stress. TRPV1 expression was reduced in 6-OHDA-treated rats, but its manipulation improved behavioral dysfunction and protected dopamine neurons. TET1 enhanced TRPV1 expression and AMPK phosphorylation, reducing neurotoxicity. The findings suggest that targeting the TET1/TRPV1 axis may provide neuroprotection in PD by activating AMPK signaling.
Products Used: EpiQuik Chromatin Immunoprecipitation (ChIP) Kit
Dong W et. al. (September 2024). Low-dose SAHA enhances CD8+ T cell-mediated antitumor immunity by boosting MHC I expression in non-small cell lung cancer Cell Oncol (Dordr).
This study investigated low-dose suberoylanilide hydroxamic acid (SAHA) and its role in enhancing CD8+ T cell-mediated antitumor immunity in non-small cell lung cancer (NSCLC). Low-dose SAHA increased MHC I expression in NSCLC cells without affecting cell viability, thereby boosting CD8+ T cell activation and cytotoxicity. The mechanism involved SAHA inhibiting histone deacetylase activity, which raised acetylation levels of STAT1, Smad2, and Smad3, leading to their increased expression and nuclear translocation. These findings suggest that low-dose SAHA may serve as a novel immunotherapy strategy for NSCLC.
Products Used: Epigenase HDAC Activity/Inhibition Direct Assay Kit (Colorimetric)
