Epigenetic Research Papers 1/8/24 - 1/12/24

He S et. al. (December 2023). NtERF4 promotes the biosynthesis of chlorogenic acid and flavonoids by targeting PAL genes in Nicotiana tabacum Planta. 259(2):31.
In this study, researchers found that NtERF4a overexpression in Nicotiana tabacum increased chlorogenic acid and flavonoid levels, while silencing had the opposite effect. NtERF4a was found to activate NtPAL1 and NtPAL2, key genes in the phenylpropanoid pathway, and contribute to stress resistance in tobacco seedlings.
Products Used: EpiQuik Chromatin Immunoprecipitation (ChIP) Kit

Takakura M et. al. (January 2024). Goji Berry Juice Prevents Tumor Necrosis Factor Alpha-Induced Xerostomia in Human Salivary Gland Cells Biol Pharm Bull. 47(1):138-144.
This study explores the preventive potential of goji berry juice (GBJ) against tumor necrosis factor alpha (TNF-α)-induced xerostomia in human salivary gland cells, highlighting its impact on aquaporin-5 (AQP-5) expression, histone H4 deacetylation, reactive oxygen species (ROS) generation, and antiapoptotic effects. The findings suggest a role for GBJ in preventing oral dryness associated with Sjögren's syndrome.
Products Used: EpiQuik Total Histone Extraction Kit, EpiQuik Total Histone H4 Acetylation Detection Fast Kit (Fluorometric)

Zhi D et. al. (December 2023). METTL3/YTHDF1 m6A axis promotes tumorigenesis by enhancing DDR2 expression in ovarian cancer Pathol Res Pract. 253:155047.
This study delves into the molecular mechanism of ovarian cancer progression, revealing that the METTL3/YTHDF1 m6A axis plays a crucial role in promoting tumorigenesis by enhancing DDR2 expression. Depletion of METTL3/YTHDF1 hindered cancer proliferation and metastasis, suggesting potential therapeutic targets for ovarian cancer treatment.
Products Used: EpiQuik m6A RNA Methylation Quantification Kit (Fluorometric), EpiQuik CUT&RUN m6A RNA Enrichment (MeRIP) Kit

Kaneshiro K et. al. (January 2024). The clock gene Bmal1 controls inflammatory mediators in rheumatoid arthritis fibroblast-like synoviocytes Biochem Biophys Res Commun. 691:149315.
This article investigates the involvement of clock genes, specifically Bmal1, in the production of inflammatory mediators from rheumatoid arthritis fibroblast-like synoviocytes (RA-FLS). Stimulation of RA-FLS with inflammatory cytokines increased Bmal1 expression, and silencing Bmal1 suppressed the production of MMP-3, CCL2, and IL-6, suggesting a regulatory role of Bmal1 in the pathogenesis of rheumatoid arthritis by modulating inflammation.
Products Used: CCL2 Polyclonal Antibody

Celis T et. al. (December 2023).  Development and validation of a human bronchial epithelial spheroid model to study respiratory toxicity in vitro Arch Toxicol.
In this study, a physiologically relevant 3D cell model consisting of human bronchial epithelial (16HBE14o-) cells was developed for respiratory toxicity research. The spheroids, formed by culturing cells as hanging drops, demonstrated increased expression of CLDN1 compared to 2D monolayer cultures, and established standard operating procedures (SOPs) were optimized for compatibility with spheroids, enabling reliable toxicity assessment of chemicals and bridging the gap between in vivo and in vitro experiments.
Products Used: MethylFlash Global DNA Methylation (5-mC) ELISA Easy Kit (Colorimetric)

Hu C et. al. (December 2023). Putrescine promotes MMP9-induced angiogenesis in skeletal muscle through hydrogen peroxide/METTL3 pathway Hu C et. al. (December 2023).
This article investigates the impact of putrescine, an essential polyamine, on skeletal muscle angiogenesis. The findings reveal that putrescine promotes angiogenesis through the hydrogen peroxide/METTL3 pathway, enhancing MMP9-induced vascular development in skeletal muscle.
Products Used: EpiQuik m6A RNA Methylation Quantification Kit (Colorimetric)