The EpiQuik™ Total Histone H4 Acetylation Detection Fast Kit (Fluorometric) is a convenient package of tools that allows the experimenter to measure general acetylation of histone H4. The kit is ready-to-use and provides all the essential components needed to carry out a successful assay experiment. The EpiQuik Total Histone H4 Acetylation Detection Fast Kit (Fluorometric) is suitable for specifically measuring total histone H4 acetylation using a variety of mammalian cells (human, mouse, etc.), including fresh and frozen tissues, and cultured adherent and suspension cells. The kit has the following advantages:
Principle & ProcedureThe EpiQuik™ Total Histone H4 Acetylation Detection Fast Kit (Fluorometric) is designed for measuring total histone H4 acetylation in a fast format. In an assay with this kit, the acetyl histone H4 is captured to the strip wells coated with an anti acetyl histone H4 antibody. The captured acetyl histone H4 can then be detected with a labeled detection antibody, followed by a fluorescent development reagent. The ratio of acetyl histone H4 is proportional to the intensity of fluorescence. The absolute amount of acetyl histone H4 can be quantified by comparing to the standard control.
Fig. 1. Schematic procedure for using the EpiQuik™ Total Histone H4 Acetylation Detection Fast Kit (Fluorometric).
Sajjanar B et. al. (August 2019). Cross-talk between energy metabolism and epigenetics during temperature stress response in C2C12 myoblasts. Int J Hyperthermia. 36(1):776-784.
Adamu HA et. al. (January 2017). In utero exposure to germinated brown rice and its oryzanol-rich extract attenuated high fat diet-induced insulin resistance in F1 generation of rats. BMC Complement Altern Med. 17(1):67.
Adamu HA et. al. (February 2016). Perinatal exposure to germinated brown rice and its gamma amino-butyric acid-rich extract prevents high fat diet-induced insulin resistance in first generation rat offspring. Food Nutr Res. 60:30209.
Ziyatdinova S et. al. (February 2015). Reduction of epileptiform activity by valproic acid in a mouse model of Alzheimer's disease is not long-lasting after treatment discontinuation Epilepsy Res. 112:43-55.