Epigenetic Research Papers 12/18/23 - 12/22/23

Qiu B et. al. (December 2023). Association between SPRY1 and TET3 in skin photoaging and natural aging mechanisms J Cosmet Dermatol.
This study explores the role of SPRY1 and TET3 in natural skin aging, replicative aging, and UVA-induced photoaging in HaCaT cells. SPRY1 expression increases with age, while TET3 expression decreases. UVA irradiation promotes cell senescence, upregulating SPRY1 and downregulating TET3. The findings suggest that SPRY1 and TET3 are key genes involved in skin aging processes, offering insights into potential interventions for age-related skin changes.
Products Used: MethylFlash Global DNA Methylation (5-mC) ELISA Easy Kit (Colorimetric)

Li L et. al. (December 2023). Maternal betaine supplementation ameliorates fatty liver disease in offspring mice by inhibiting hepatic NLRP3 inflammasome activation Nutr Res Pract. 17(6):1084-1098.
This study explores how maternal betaine supplementation affects fatty liver disease (FLD) in offspring mice. Maternal betaine intake significantly alleviates hepatic steatosis, reduces inflammatory markers, and inhibits NLRP3 inflammasome activation in the livers of offspring mice exposed to a high-fat diet and streptozotocin. The findings highlight the potential anti-inflammatory and lipotropic effects of maternal betaine supplementation in preventing FLD.
Products Used: MethylFlash Global DNA Methylation (5-mC) ELISA Easy Kit (Colorimetric)

Guardiola O et. al. (November 2023).  CRIPTO-based micro-heterogeneity of mouse muscle satellite cells enables adaptive response to regenerative microenvironment Dev Cell.
The research paper investigates how non-genetic micro-heterogeneity in the membrane protein CRIPTO influences mouse muscle satellite cells during regeneration. CRIPTO micro-heterogeneity allows rapid adaptive responses to environmental changes through intracellular processes. Disrupting CRIPTO micro-heterogeneity alters satellite cell behavior, affecting proliferation and self-renewal, with increased self-renewal observed in vivo. The findings emphasize the pivotal role of CRIPTO micro-heterogeneity in regulating adaptive responses during skeletal muscle repair.
Products Used: MethylFlash Global DNA Hydroxymethylation (5-hmC) ELISA Easy Kit (Colorimetric)

Sugawara H et. al. (December 2023). Quantification of cytosine modifications in the aged mouse brain Neuropsychopharmacol Rep.
The study quantifies cytosine modifications in the aged mouse brain, revealing consistent regional variations across development. Positive correlations between 5-mC and 5-hmC levels differ by region, while 5-fC levels show no correlation. An age-related decline in 5-fC levels in the basal ganglia suggests a unique epigenetic regulation mechanism, emphasizing the need for additional research in neuropsychiatric disorder models and postmortem brain analysis.
Products Used: MethylFlash 5-Formylcytosine (5-fC) DNA Quantification Kit (Colorimetric)

Yu H et. al. (December 2023). Transcription factor OsMYB30 increases trehalose content to inhibit α-amylase and seed germination at low temperature Plant Physiol.
The article investigates the molecular basis of low-temperature germination (LTG) in rice. OsMYB30 and OsTPP1 were identified as key regulators in response to cold stress. Overexpression of either gene delayed germination and heightened cold sensitivity. OsMYB30 was found to bind to the OsTPP1 promoter, increasing trehalose levels and inhibiting α-amylase activity during seed germination. These findings unveil a phytohormone-independent pathway that integrates environmental cues with internal factors to control LTG in rice.
Products Used: EpiQuik Plant ChIP Kit

Li X et. al. (December 2023). Non-canonical STING-PERK pathway dependent epigenetic regulation of vascular endothelial dysfunction via integrating IRF3 and NF- κ B in inflammatory response Acta Pharm Sin B. 13(12):4765-4784.
This research explores the non-canonical STING-PERK pathway in atherosclerosis, finding that it enhances BRD4 expression and promotes inflammation. Endothelial deficiencies in STING and BRD4 reduce plaque and inflammation, offering a potential therapeutic target for atherosclerosis-induced vascular dysfunction.
Products Used: EpiQuik 8-OHdG DNA Damage Quantification Direct Kit (Colorimetric)

Zhang LS et. al. (December 2023).  Base-Resolution Sequencing Methods for Whole-Transcriptome Quantification of mRNA Modifications Acc Chem Res.
This article discusses advanced methods for quantifying mRNA modifications at a whole-transcriptome level. Emphasizing chemical modifications beyond m6A, the study presents chemistry-based techniques, including m6A-SAC-seq, eTAM-seq, BID-seq, UBS-seq, DAMM-seq, m1A-quant-seq, Nm-Mut-seq, and m7G-quant-seq. These methods offer base-resolution mapping of diverse mRNA modifications with low input, enabling insights into their dynamic changes during biological processes and potential applications as clinical biomarkers.
Products Used: Methylamp RNA Bisulfite Conversion Kit

Wang L et. al. (March 2024). METTL3-mediated lncRNA HOXD-AS1 stability regulates inflammation, and the migration and invasion of trophoblast cells via the miR-135a/ β-TRCP axis Noncoding RNA Res. 9(1):12-23.
This study investigates METTL3-mediated lncRNA HOXD-AS1 in preeclampsia. Elevated METTL3, HOXD-AS1, and β-TRCP in PE placental tissues, along with reduced miR-135a, indicate their involvement. HOXD-AS1 targets miR-135a, regulated by METTL3. Overexpression of METTL3, HOXD-AS1, and β-TRCP, coupled with miR-135a knockdown, inhibits trophoblast cell functions and increases inflammatory factors. The study identifies a regulatory axis involving miR-135a, β-TRCP, and NF-κB pathway influenced by METTL3 and HOXD-AS1 in PE.
Products Used: EpiQuik m6A RNA Methylation Quantification Kit (Colorimetric)

Meng Y et. al. (November 2023). KIAA1429 facilitates progression of hepatocellular carcinoma by modulating m6A levels in HPN Heliyon. 9(11):e22084.
This study explores the role of KIAA1429, a major m6A methyltransferase, in liver hepatocellular carcinoma (LIHC). Elevated expression of KIAA1429 is observed in LIHC tissues, correlating with poor prognosis. Silencing KIAA1429 attenuates LIHC metastasis and proliferation. KIAA1429 is identified to regulate m6A levels in HPN, contributing to LIHC progression. The study proposes a KIAA1429-HPN modulatory model based on m6A modifications, providing insights into LIHC occurrence and development.
Products Used: EpiQuik m6A RNA Methylation Quantification Kit (Colorimetric)

Kim H et. al. (December 2023). Phylogenetic and functional analyses of N6-methyladenosine RNA methylation factors in the wheat scab fungus Fusarium graminearum mSphere. :e0055223.
The article investigates m6A RNA methylation factors in Fusarium graminearum, revealing their distribution and functions in fungi. It identifies copy number variations, providing insights into m6A machinery evolution. Functional analysis emphasizes the crucial role of m6A writer MTA1 in conidial germination. The research advances our understanding of m6A modification in fungi and its potential applications in disease control.
Products Used: EpiQuik m6A RNA Methylation Quantification Kit (Colorimetric)