Epigenetic Research Papers 1/1/24 - 1/5/24

Zhang Y et. al. (December 2023). Histone H3 Acetylation Is Involved in Retinoid Acid-Induced Neural Differentiation through Increasing Mitochondrial Function Biomedicines. 11(12)
This study, using the SH-SY5Y cell line, demonstrates that RA promotes neural differentiation by enhancing H3K14ac and mitochondrial function, with RA receptors (RARs) and the co-repressor RIP140 playing key regulatory roles in this molecular process, offering insights into abnormal neural development mechanisms.
Products Used: EpiQuik Nuclear Extraction Kit, EpiQuik Total Histone Extraction Kit, EpiQuik HAT Activity/Inhibition Assay Kit

Liu Y et. al. (December 2023). The Effects of Larval Cryopreservation on the Epigenetics of the Pacific Oyster Crassostrea gigas Int J Mol Sci. 24(24)
In this study, the impact of larval cryopreservation on the epigenetics of Pacific oyster Crassostrea gigas progenies was investigated. The results revealed dynamic changes in DNA methylation levels and the expression of epigenetic regulator genes, suggesting a short-term effect of the cryopreservation technique on post-thaw larvae in C. gigas.
Products Used: EpiQuik Total Histone Extraction Kit, MethylFlash Methylated DNA Quantification Kit (Fluorometric)

Zang S et. al. (December 2023). FTO-mediated m6A demethylation regulates GnRH expression in the hypothalamus via the PLCβ3/Ca2+/CAMK signalling pathway Commun Biol. 6(1):1297.
The article explores the role of N6-methyladenosine (m6A) in the development and functional homeostasis of the central nervous system, with a specific focus on the fat mass and obesity-associated (FTO) gene in the hypothalamus. The study reveals that FTO regulates GnRH expression, influencing puberty onset in female rats, by orchestrating m6A demethylation of PLCβ3 and activating the Ca2+ signalling pathway.
Products Used: EpiQuik m6A RNA Methylation Quantification Kit (Colorimetric), EpiQuik CUT&RUN m6A RNA Enrichment (MeRIP) Kit

Holtz AG et. al. (December 2023). Asymmetric and symmetric protein arginine methylation in methionine-addicted human cancer cells PLoS One. 18(12):e0296291.
The article investigates the role of protein arginine methylation, specifically asymmetric dimethylarginine (ADMA) and symmetric dimethylarginine (SDMA), in methionine-addicted human cancer cells. The study, comparing methionine-addicted and less methionine-dependent cancer cell lines, suggests that alterations in protein arginine methylation may not be the primary contributors to the Hoffman effect, highlighting the importance of other consequences of methionine addiction in cancer metastasis and malignancy.
Products Used: EpiQuik Total Histone Extraction Kit

Cheng Z et. al. (January 2024). Proteomic analysis identifies PFKP lactylation in SW480 colon cancer cells iScience. 27(1):108645.
The study explores lysine lactylation, a post-translational modification, in FHC and SW480 colon cancer cells through mass spectrometry. A total of 637 lysine lactylation sites in 444 proteins were identified, with enrichment in the glycolysis pathway, revealing PFKP lactylation as a potential negative feedback mechanism in glycolysis and lactic acid production in SW480 colon cancer cells.
Products Used: EpiQuik Total Histone Extraction Kit

Ghaffarinia A et. al. (December 2023). Unraveling Transcriptome Profile, Epigenetic Dynamics, and Morphological Changes in Psoriasis-like Keratinocytes: "Insights into Similarity with Psoriatic Lesional Epidermis" Cells. 12(24)
The study investigates the impact of psoriasis-like inflammation on cultured keratinocytes, revealing morphological changes, transcriptome alterations, and epigenetic dynamics. The keratinocytes under mild inflammation show a closer resemblance to the psoriatic epidermis transcriptome, with IL-17 pathway genes playing a significant role, while both mild and severe inflammation lead to the regulation of epigenetic modifiers, emphasizing the complex interplay between inflammation severity, transcriptomic changes, and epigenetic regulation in psoriasis.
Products Used: 5-Methylcytosine (5-mC) Monoclonal Antibody [33D3]

Takeda Y et. al. (December 2023). Epigenetic alterations of 11beta-hydroxysteroid dehydrogenase 1 gene in the adipose tissue of patients with primary aldosteronism Endocr J.
The article explores epigenetic alterations in the 11beta-hydroxysteroid dehydrogenase 1 (11β-HSD1) gene in the adipose tissue of patients with primary aldosteronism (PA). The findings reveal differences in the methylation status and mRNA levels of HSD11B1, suggesting a potential link between epigenetic regulation and the pathophysiology of PA in human adipose tissue.
Products Used: MethylFlash Methylated DNA Quantification Kit (Fluorometric)

Kharboush TG et. al. (December 2023). Epigenetic alterations of miR-155 and global DNA methylation as potential mediators of ochratoxin A cytotoxicity and carcinogenicity in human lung fibroblasts Environ Sci Pollut Res Int.
This study investigates the potential carcinogenicity of ochratoxin A (OTA) in human lung fibroblasts (WI-38 cells) and explores the underlying molecular mechanisms. The study reveals that OTA induces cytotoxicity in a dose-dependent manner, with significant epigenetic alterations such as global DNA hypermethylation and upregulation of miR-155, suggesting their involvement in OTA toxicity and potential carcinogenic effects in lung fibroblast cells.
Products Used: MethylFlash Methylated DNA Quantification Kit (Fluorometric)

Zhao T et. al. (December 2023). N6-methyladenosine promotes aberrant redox homeostasis required for arsenic carcinogenesis by controlling the adaptation of key antioxidant enzymes J Hazard Mater. 465:133329.
The study explores the role of N6-methyladenosine (m6A), an RNA epigenetic modification, in arsenic carcinogenesis and aberrant redox homeostasis. The research reveals that arsenic-induced reactive oxygen species (ROS) elevate m6A levels, leading to m6A-dependent adaptive expressions of key antioxidant enzymes, and disrupting this m6A balance using inhibitors results in disturbed redox homeostasis and cell death in arsenic-induced tumors.
Products Used: EpiQuik m6A RNA Methylation Quantification Kit (Colorimetric)