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SuperSense Methylated DNA Quantification Kit

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Schematic procedure for the SuperSense Methylated DNA Quantification Kit.
Quantification of methylated DNA using the fluorometric SuperSense Methylated DNA Quantification Kit.
Input Type: DNA
Research Area: DNA Methylation
Target Application: Amount Quantitation
Vessel Format: 96-Well Plate
100% Guarantee: 6 months
Catalog No.SizePriceQty
P-1021-4848 assays $359.00 
P-1021-9696 assays $619.00 
Order now & get it by Friday, December 13th  
Product Overview





We recommend using a newer version of this product: MethylFlash Methylated DNA Quantification Kit (Fluorometric).



The SuperSense™ Methylated DNA Quantification Kit is a complete set of essential components which enables the experimenter to fluorometrically measure levels of methylated DNA using a small amount of DNA samples. The SuperSense™ Methylated DNA Quantification Kit is suitable for detecting global DNA methylation status using genomic DNA isolated from any species such as mammal, plant, fungus, bacteria and virus in a variety of forms including cultured cells, fresh and frozen tissues, paraffin-embedded tissues, plasma/serum samples, and body fluid samples, etc.

WHY CHOOSE THE SUPERSENSE™ METHYLATED DNA QUANTIFICATION KIT,

  • Fluorescence-based assay with easy-to-follow steps for convenience and speed. The whole procedure can be finished within 2 hours and 30 minutes.
  • High sensitivity, of which detection limit can be as low as 50 pg of methylated DNA with the input DNA being as low as 2 ng.
  • Universal positive control, which is suitable for quantifying methylated DNA from any species.
  • Strip microplate format makes the assay flexible: manual or high throughput analysis.
Product Details

The SuperSense™ Methylated DNA Quantification Kit contains all reagents required for fluorescent quantification of global DNA methylation. In this assay, DNA is immobilized to the strip well specifically coated with a DNA affinity substance. The methylated DNA can be recognized with anti-5-methylcytosine antibody. The ratio or amount of methylated DNA, which is proportional to fluorescence intensity, can then be fluorometrically quantified.

SCHEMATIC PROCEDURE:  



Quantification of methylated DNA using the fluorometric SuperSense Methylated DNA Quantification Kit.
User Guide & MSDS

[User Guide]*
*Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing info@epigentek.com along with your contact information and institution name.

[Safety Data Sheet]
Product Citations

Geyer KK et. al. (May 2017). The Biomphalaria glabrata DNA methylation machinery displays spatial tissue expression, is differentially active in distinct snail populations and is modulated by interactions with Schistosoma mansoni. PLoS Negl Trop Dis. 11(5):e0005246.

Ohashi K et. al. (October 2015). High fructose consumption induces DNA methylation at PPARĪ± and CPT1A promoter regions in the rat liver. Biochem Biophys Res Commun.

Shankar TV et. al. (June 2015). N-Hydroxy-6-(5-NitroNaphtalimide)-Hexanamide Inhibits Lysine Deacetylation, Mitigates Angiogenesis and Reduces Tumor Growth J Cancer Sci. 2(1):11.

Wong CP et. al. (February 2015). Zinc deficiency enhanced inflammatory response by increasing immune cell activation and inducing IL6 promoter demethylation. Mol Nutr Food Res.

Xie Q et. al. (May 2014). Genistein inhibits DNA methylation and increases expression of tumor suppressor genes in human breast cancer cells. Genes Chromosomes Cancer. 53(5):422-31.

Fullston T et. al. (October 2013). Paternal obesity initiates metabolic disturbances in two generations of mice with incomplete penetrance to the F2 generation and alters the transcriptional profile of testis and sperm microRNA content. FASEB J. 27(10):4226-43.

Padmanabhan N et. al. (September 2013). Mutation in folate metabolism causes epigenetic instability and transgenerational effects on development. Cell. 155(1):81-93.

Geyer KK et. al. (July 2013). Cytosine methylation is a conserved epigenetic feature found throughout the phylum Platyhelminthes. BMC Genomics. 14:462.

Wong CP et. al. (January 2013). Increased inflammatory response in aged mice is associated with age-related zinc deficiency and zinc transporter dysregulation. J Nutr Biochem. 24(1):353-9.

Wong CP et. al. (January 2013). Increased inflammatory response in aged mice is associated with age-related zinc deficiency and zinc transporter dysregulation. J Nutr Biochem. 24(1):353-9.

Wong CP et. al. (September 2011). Induction of regulatory T cells by green tea polyphenol EGCG. Immunol Lett. 139(1-2):7-13.

Geyer KK et. al. (August 2011). Cytosine methylation regulates oviposition in the pathogenic blood fluke Schistosoma mansoni. Nat Commun. 2:424.

Cho YH et. al. (July 2011). Identification of transcriptional regulatory elements required for the Mup2 expression in circadian clock mutant mice. Biochem Biophys Res Commun. 410(4):834-40.

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