We recommend using a newer version of this product: MethylFlash Methylated DNA Quantification Kit (Fluorometric).
The SuperSense™ Methylated DNA Quantification Kit is a complete set of essential components which enables the experimenter to fluorometrically measure levels of methylated DNA using a small amount of DNA samples. The SuperSense™ Methylated DNA Quantification Kit is suitable for detecting global DNA methylation status using genomic DNA isolated from any species such as mammal, plant, fungus, bacteria and virus in a variety of forms including cultured cells, fresh and frozen tissues, paraffin-embedded tissues, plasma/serum samples, and body fluid samples, etc.
WHY CHOOSE THE SUPERSENSE™ METHYLATED DNA QUANTIFICATION KIT,
- Fluorescence-based assay with easy-to-follow steps for convenience and speed. The whole procedure can be finished within 2 hours and 30 minutes.
- High sensitivity, of which detection limit can be as low as 50 pg of methylated DNA with the input DNA being as low as 2 ng.
- Universal positive control, which is suitable for quantifying methylated DNA from any species.
- Strip microplate format makes the assay flexible: manual or high throughput analysis.
The SuperSense™ Methylated DNA Quantification Kit contains all reagents required for fluorescent quantification of global DNA methylation. In this assay, DNA is immobilized to the strip well specifically coated with a DNA affinity substance. The methylated DNA can be recognized with anti-5-methylcytosine antibody. The ratio or amount of methylated DNA, which is proportional to fluorescence intensity, can then be fluorometrically quantified.
Quantification of methylated DNA using the fluorometric SuperSense Methylated DNA Quantification Kit.
SA1 (10X Wash Buffer)
SA2 (DNA Binding Solution)
SA3 (Positive Control, 100 µg/ml)**
SA4 (Block Solution)
SA5 (Capture Antibody, 1000 µg/ml)*
SA6 (Labeling Solution)*
SA7 (Fluoro Developer)*
SA8 (Fluoro Enhancer)*
SA9 (Fluoro Dilution)
8-Well Assay Strips (with Frame)
* Spin the solution down to the bottom before use.
** This control is synthesized polynucleotide methylated at every 5-cytosine.