SuperSense Methylated DNA Quantification Kit

We recommend using a newer version of this product: MethylFlash Methylated DNA Quantification Kit (Fluorometric).

The SuperSense™ Methylated DNA Quantification Kit is a complete set of essential components which enables the experimenter to fluorometrically measure levels of methylated DNA using a small amount of DNA samples. The SuperSense™ Methylated DNA Quantification Kit is suitable for detecting global DNA methylation status using genomic DNA isolated from any species such as mammal, plant, fungus, bacteria and virus in a variety of forms including cultured cells, fresh and frozen tissues, paraffin-embedded tissues, plasma/serum samples, and body fluid samples, etc.

WHY CHOOSE THE SUPERSENSE™ METHYLATED DNA QUANTIFICATION KIT,

• Fluorescence-based assay with easy-to-follow steps for convenience and speed. The whole procedure can be finished within 2 hours and 30 minutes.
• High sensitivity, of which detection limit can be as low as 50 pg of methylated DNA with the input DNA being as low as 2 ng.
• Universal positive control, which is suitable for quantifying methylated DNA from any species.
• Strip microplate format makes the assay flexible: manual or high throughput analysis.

The SuperSense™ Methylated DNA Quantification Kit contains all reagents required for fluorescent quantification of global DNA methylation. In this assay, DNA is immobilized to the strip well specifically coated with a DNA affinity substance. The methylated DNA can be recognized with anti-5-methylcytosine antibody. The ratio or amount of methylated DNA, which is proportional to fluorescence intensity, can then be fluorometrically quantified.

SCHEMATIC PROCEDURE:
	Quantification of methylated DNA using the fluorometric SuperSense Methylated DNA Quantification Kit.