BackgroundThe Silent Information Regulator (SIR2) family of genes is a highly conserved group of genes that encode nicotinamide adenine dinucleotide (NAD)-dependent protein deacetylases, also known as class III histone deacetylases. The first discovered and best characterized of these genes is Saccharomyces cerevisiae SIR2, which is involved in silencing of mating type loci, telomere maintenance, DNA damage response, and cell aging. SirT1, the mammalian ortholog of Sir2, is a nuclear protein implicated in the regulation of many cellular processes, including apoptosis, cellular senescence, endocrine signaling, glucose homeostasis, aging, and longevity. Targets of SirT1 include acetylated p53, p300, Ku70, forkhead (FoxO) transcription factors, PPARγ, and the PPARγ coactivator-1α (PGC-1α) protein. Deacetylation of p53 and FoxO transcription factors represses apoptosis and increases cell survival. Deacetylation of PPARγ and PGC-1α regulates the gluconeogenic/glycolytic pathways in the liver and fat mobilization in white adipocytes in response to fasting. SirT1 deacetylase activity is inhibited by nicotinamide and activated by resveratrol. In addition, SirT1 activity may be regulated by phosphorylation, since it is phosphorylated on Ser27 and Ser47 in vivo, however, the function of these phosphorylation sites has not yet been determined.
DescriptionMouse monoclonal antibody raised against a partial recombinant SIRT1, clone 7B7.
Size100 µg
PurificationProtein A purified
ImmunogenSIRT1 (AAH12499, 456 a.a. ~ 555 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Cross ReactivityHuman
IsotypeIgG1 Kappa
FormulationIn 1x PBS, pH 7.2
StorageAliquot and store at -20ºC or lower. Avoid repeated freezing and thawing. Multiple freeze/thaw cycles may result in decreased performance.
Handling RecommendationsFor maximum recovery of the products, centrifuge the vial prior to opening the cap.
Alternative NamesSirtuin1, 75SirT1 antibody, BA57G10.4 antibody, hSIR2 antibody, hSIRT1 antibody, NAD dependent deacetylase SIRT1 antibody, NAD dependent deacetylase sirtuin 1 antibody, NAD dependent protein deacetylase sirtuin 1 antibody, OTTHUMP00000198111 antibody, OTTHUMP00000198112 antibody, Regulatory protein SIR2 homolog 1 antibody, SIR2 like 1 antibody, SIR2ALPHA antibody, SIR2alpha protein antibody, SIR2L1 antibody, SirtT1 75 kDa fragment antibody, sirtuin (silent mating type information regulation 2 homolog) 1 (S. cerevisiae) antibody, sirtuin antibody
Applications & Suggested Dilutions*Western Blot: 0.5-1 µg/mlELISA: 0.25-1 µg/mlImmunohistochemistry (IHC): 2.5 µg/mlImmunofluorescence: 5 µg/ml