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SET7 Monoclonal Antibody [5B4]

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Application: ELISA, IF, IHC, WB
Areas of Research: Histone Methylation & Demethylation
Clonality: Monoclonal
Conjugate: Unconjugated
Host: Mouse
Isotype: IgG2a Kappa
Purification: Affinity Purified
Reactivity: Human
100% Guarantee: 6 months
Catalog No.SizePriceQty
A-3102-100100 µg $452.00  Discontinued
Why EpiGentek AntibodiesFree Shipping on Antibodies
Availability: Discontinued 
Product Overview

Background
SET7, also known as SET 7/9, is a histone methyltransferase that specifically methylates histone H3 Lys4 (H3K4) and contains a SET domain. It acts on several non-histone as well as histone targets such as TAF10, histone H3, p53, and estrogen receptor alpha. SET7 is implicated in numerous human diseases due to its broad substrate specificity and researchers use SET7/9 as a model for investigating the mechanism of SET domain histone methyltransferases.  

Description
Mouse monoclonal antibody raised against a partial recombinant SET7, clone 5B4.

Size
100 µg

Purification
Protein A purified

Immunogen
SET7 (NP_085151, 257 a.a. ~ 367 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Cross Reactivity
Human

Isotype
IgG2a Kappa

Formulation
In 1x PBS, pH 7.2

Storage
Aliquot and store at -20ºC or lower. Avoid repeated freezing and thawing. Multiple freeze/thaw cycles may result in decreased performance.

Handling Recommendations
For maximum recovery of the products, centrifuge the vial prior to opening the cap.

Alternative Names
H4 K20 HMTase antibody, Histone H4 K20 methyltransferase antibody, Histone lysine N methyltransferase H4 lysine 20 specific antibody, Lysine N-methyltransferase 5A antibody, N-lysine methyltransferase SETD8 antibody, Pr SET 7 antibody, PR/SET domain containing protein 07 antibody, PR/SET domain containing protein 8 antibody, SET 8 antibody, SET domain containing (lysine methyltransferase) 8 antibody, SET8 antibody, SET9 antibody, SETD 8 antibody

Applications & Suggested Dilutions*
Western Blot: 0.5-1 μg/ml
ELISA: 0.25-1 μg/ml
Immunohistochemistry (IHC): 2.5 μg/ml
Immunofluorescence: 5 μg/ml

*The end user is responsible for determining optimal working dilutions.

Quality Control

Western Blot detection against Immunogen (38.21 KDa).

 

Note: This item was formerly catalog no. ABB-3002 (ABB3002) 

Product Details

Applications

Western Blot



SET7 Monoclonal Antibody [5B4]. Western Blot analysis of SET7 expression in Jurkat.

Immunoprecipitation

Not determined
ELISA Applicable

Immunohistochemistry (IHC)


Immunoperoxidase of monoclonal antibody to SET7 on formalin-fixed paraffin-embedded human colon. [Antibody Concentration: 0.5 μg/ml]
Immunofluorescence  

Immunofluorescence of monoclonal antibody to SET7 on HeLa cell. [Antibody Concentration: 10 µg/ml]
User Guide & MSDS

[User Guide]*
*Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing info@epigentek.com along with your contact information and institution name.

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