BackgroundSET7, also known as SET 7/9, is a histone methyltransferase that specifically methylates histone H3 Lys4 (H3K4) and contains a SET domain. It acts on several non-histone as well as histone targets such as TAF10, histone H3, p53, and estrogen receptor alpha. SET7 is implicated in numerous human diseases due to its broad substrate specificity and researchers use SET7/9 as a model for investigating the mechanism of SET domain histone methyltransferases.
DescriptionMouse monoclonal antibody raised against a partial recombinant SET7, clone 5B4.
Size100 µg
PurificationProtein A purified
ImmunogenSET7 (NP_085151, 257 a.a. ~ 367 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Cross ReactivityHuman
IsotypeIgG2a Kappa
FormulationIn 1x PBS, pH 7.2
StorageAliquot and store at -20ºC or lower. Avoid repeated freezing and thawing. Multiple freeze/thaw cycles may result in decreased performance.
Handling RecommendationsFor maximum recovery of the products, centrifuge the vial prior to opening the cap.
Alternative NamesH4 K20 HMTase antibody, Histone H4 K20 methyltransferase antibody, Histone lysine N methyltransferase H4 lysine 20 specific antibody, Lysine N-methyltransferase 5A antibody, N-lysine methyltransferase SETD8 antibody, Pr SET 7 antibody, PR/SET domain containing protein 07 antibody, PR/SET domain containing protein 8 antibody, SET 8 antibody, SET domain containing (lysine methyltransferase) 8 antibody, SET8 antibody, SET9 antibody, SETD 8 antibody
Applications & Suggested Dilutions*Western Blot: 0.5-1 μg/mlELISA: 0.25-1 μg/mlImmunohistochemistry (IHC): 2.5 μg/mlImmunofluorescence: 5 μg/ml
*The end user is responsible for determining optimal working dilutions.
Quality ControlWestern Blot detection against Immunogen (38.21 KDa).
Note: This item was formerly catalog no. ABB-3002 (ABB3002)
Applications
Western Blot
SET7 Monoclonal Antibody [5B4]. Western Blot analysis of SET7 expression in Jurkat.
Immunoprecipitation
Immunohistochemistry (IHC)