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PPP2CA Polyclonal Antibody

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Western Blot
Positive WB detected in: Mouse liver tissue, Mouse kidney tissue
All lanes: PPP2CA Polyclonal Antibody at 2.5ug/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 36, 30 KDa
Observed band size: 36, 30 KDa
Immunohistochemistry of paraffin-embedded human liver cancer using PPP2CA Polyclonal Antibody at dilution of 1:100.
Immunofluorescent analysis of Hela cells using PPP2CA Polyclonal Antibody at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
Application: ELISA, IF, IHC
Areas of Research: Signal Transduction
Clonality: Polyclonal
Conjugate: Unconjugated
Host: Rabbit
Isotype: IgG
Purification: >95%, Protein G Purified
Reactivity: Human
Trial Size Available: Yes
100% Guarantee: 6 months
Catalog No.SizePriceQty
A63206-02020 µg $99.00 
A63206-05050 µg $256.00 
A63206-100100 µg $359.00 
Availability: Usually Ships Within 1-2 Weeks 
Product Overview

Background
PP2A is the major phosphatase for microtubule-associated proteins (MAPs). PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. Cooperates with SGOL2 to protect centromeric cohesin from separase-mediated cleavage in oocytes specifically during meiosis I. Can dephosphorylate SV40 large T antigen and p53/TP53. Activates RAF1 by dephosphorylating it at 'Ser-259'.

Description
PPP2CA Polyclonal Antibody. Unconjugated. Raised in: Rabbit.

Formulation
Liquid. 0.03% Proclin 300, 50% Glycerol, 0.01M PBS, PH 7.4.

Specificity
Human

Isotype
IgG

Uniprot ID
P67775

Purification
>95%, Protein G purified

Immunogen
Recombinant Human Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform protein (1-309AA)

Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C (short-term) or -80°C (long-term). Avoid repeated freeze.

Alternative Names
Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform, PP2A-alpha, Replication protein C, RP-C, PPP2CA

Application
ELISA, IHC, IF; Recommended dilution: IHC:1:20-1:200, IF:1:50-1:200

User Guide & MSDS

[User Guide]*
*Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing [email protected] along with your contact information and institution name.

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