We recommend our top selling Histone H4 Modification Multiplex Assay Kit to quantify 10 different histone H4 modifications.
BackgroundHistones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated; this record represents the centromeric copy.
DescriptionHistone H4R3 Dimethyl Asymmetric (H4R3me2a) Polyclonal Antibody. Unconjugated. Raised in: Rabbit.
FormulationPBS with 0.02% sodium azide, 50% glycerol, pH7.3.
SpecificityHuman, Mouse, Rat, Broad Range
IsotypeIgG
Uniprot IDP62805
PurificationAffinity Purified
ImmunogenSynthetic Peptide of Human Asymmetric DiMethyl-Histone H4-R3
StorageShipped at 4°C. Store at -20°C. Avoid multiple freeze/thaw cycles.
Alternative NamesH4R3me2a, H3t, H3.4, H3/g, H3FT, H4 Arginine 3 me2a
ApplicationWB, IHC, IF, IP; Recommended dilutions: WB 1:500 - 1:2000, IHC 1:50 - 1:200, IF 1:50 - 1:200, IP 1:50 - 1:100