BackgroundThe primary building block of chromatin is the nucleosome which is made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3, and H4). Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated through an intricate series of post-translational modifications of histones, known as the histone code, and nucleosome remodeling. Histone methylation and demethylation is dynamically regulated by histone methyl transferases and histone demethylases respectively. Methylation of histone H3K9 is associated with gene repression.
DescriptionMouse monoclonal antibody to Histone H3K9, Clone 6F12H4, ChIP-grade
FormulationLiquid. In PBS containing 0.02% sodium azide.
ImmunogenBranched peptide of the sequence (QTARK(Me)3STGGKA)2-KC
SpecificityHuman, Mouse
IsotypeIgG1
PurificationSerum
StorageStore at -4°C for short term. Aliquot and store at -20°C for long term. Avoid multiple freeze/thaw cycles.
Alternative NamesH3K9me3, Trimethyl-histone H3 (Lys9)
ApplicationELISA, ICC, WB, ChIP