BackgroundHistones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher-order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
DescriptionHistone H3K4me3 (H3K4 Trimethyl) Monoclonal Antibody, clone 9A10D4. Unconjugated. Raised in: Rabbit.
FormulationBuffer: PBS with 0.05% proclin 300, 0.05% BSA, 50% glycerol, pH7.3.
SpecificityHuman, Mouse, Rat, Broad Range
IsotypeIgG
Uniprot IDQ16695, P68431
PurificationAffinity Purified
ImmunogenRecombinant protein of human TriMethyl-Histone H3-K4
StorageShipped at 4°C. Upon receipt, store at -20°C. Avoid freeze / thaw cycles
Alternative NamesH3.4; H3/g; H3FT; H3t; HIST3H3; Histone H3; HIST1H3A
ApplicationWB, IHC, IF/ICC, IP, ChIP, DB; Recommended dilution: WB 1:10000 - 1:53000, IHC 1:50 - 1:200, IF/ICC 1:50 - 1:200, ChIP 1:50 - 1:200, DB 1:500 - 1:2000
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