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HDAC4 Monoclonal Antibody [2B11]

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Application: ELISA, IHC, WB
Areas of Research: Histone Acetylation & Deacetylation
Clonality: Monoclonal
Conjugate: Unconjugated
Host: Mouse
Isotype: IgG2a Kappa
Purification: Affinity Purified
Reactivity: Human
100% Guarantee: 6 months
Catalog No.SizePriceQty
A-4104-100100 µg $452.00  Discontinued
Availability: Discontinued 
Product Overview

Background
Histone deacetylases (HDACs) play a critical role in transcriptional repression of gene expression in eukaryotic cells through catalyzing the hydrolytic removal of acetyl groups from histone lysine residues. Three distinct families of HDACs have been described, comprising a group of at least 20 proteins in humans. HDAC4 is a class I histone deacetylase. 

Description
Mouse monoclonal antibody raised against a partial recombinant HDAC4, clone 2B11.

Size
100 µg

Purification
Protein A purified

Immunogen
HDAC4 (NP_006028, 551 a.a. ~ 648 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Cross Reactivity
Human

Isotype
IgG2a Kappa

Formulation
In 1x PBS, pH 7.2

Storage
Aliquot and store at -20ºC or lower. Avoid repeated freezing and thawing. Multiple freeze/thaw cycles may result in decreased performance.

Handling Recommendations
For maximum recovery of the products, centrifuge the vial prior to opening the cap.

Alternative Names
AHO3 antibody, BDMR antibody, EC 3.5.1.98 antibody, HA6116 antibody, HD 4 antibody, HDACA antibody, Histone Deacetylase 4 antibody, Histone Deacetylase A antibody, KIAA0288 antibody

Applications & Suggested Dilutions*
Western Blot: 0.5-1 µg/ml
ELISA: 0.25-1 µg/ml
Immunohistochemistry (IHC): 2.5 µg/ml

*The end user is responsible for determining optimal working dilutions.

Quality Control

Western Blot detection against Immunogen (36.89 KDa).

 

Note: This item was formerly catalog no. ABB-4004 (ABB4004)

Product Details

Applications

Western Blot


HDAC4 Monoclonal Antibody [2B11]. Western Blot analysis of HDAC4 expression in Jurkat.

Immunoprecipitation Not determined

ELISA

 
Detection limit for recombinant GST tagged HDAC4 is approximately 0.1 ng/ml as a capture antibody.

Immunohistochemistry (IHC)

 
Immunoperoxidase of monoclonal antibody to HDAC4 on formalin-fixed paraffin-embedded human spleen. [Antibody Concentration: 3 µg/ml]
Immunofluorescence Not determined
User Guide & MSDS

[User Guide]*
*Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing info@epigentek.com along with your contact information and institution name.

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