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H2AX Polyclonal Antibody

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Western blot analysis of extracts of various cell lines, using H2AX Polyclonal Antibody.
Western blot analysis of extracts of various cell lines, using H2AX Polyclonal Antibody.
Application: ICC, IHC, WB
Areas of Research: Unmodified Histone
Clonality: Polyclonal
Conjugate: Unconjugated
Host: Rabbit
Isotype: IgG
Purification: Affinity Purified
Reactivity: Human, Mouse, Rat
100% Guarantee: 6 months
Catalog No.SizePriceQty
A-7701-05050 µl $299.00 
A-7701-100100 µl $550.00 
Availability: Usually Ships in 3-4 Days 
Product Overview

Background
Histone H2A. X is a variant histone of H2A and is necessary for DNA repair and checkpoint-mediated cell cycle arrest that occurs after double-stranded DNA breaks. DNA damage from ionizing radiation, radiomimetic agents, or UV-light causes rapid phosphorylation of H2A. X at Ser139 via PI3K-like kinases. Very soon after DNA damage occurs, H2A. X is phosphorylated at Ser139 at sites of DNA damage. This is required for the recruitment of DNA-damage response proteins, such as MDC1, NBS1, RAD50, MRE11, 53BP1, and BRCA1. H2A. X is also involved in the fragmentation of DNA during apoptosis and, in response to apoptotic signals, is phosphorylated by various kinases. H2A. X can be phosphorylated at Ser139 by DNA-PK, c-Jun N-terminal Kinase (JNK1), and p38 MAPK. In undamaged cells, H2A. X is phosphorylated on Tyr142 by WSTF (Williams-Beuren syndrome transcription factor). Tyr142 is dephosphorylated at DNA damage sites by EYA1 and EYA3 phosphatases. This happens as soon as DNA damage and phosphorylation of Ser139 occur. Phosphorylation at Ser139 helps recruit DNA repair and apoptotic proteins to DNA damage sites, and phosphorylation at Tyr142 determines which set of proteins are recruited. H2A. X phosphorylation at Tyr142 inhibits the enlistment of DNA repair proteins and endorses binding of pro-apoptotic factors such as JNK1. Fibroblasts studied in mouse embryos that only express mutant H2A. X Y142F display diminished apoptotic response to ionizing radiation. This suggests that the balance of H2A. X Tyr142 phosphorylation and dephosphorylation acts as a switch mechanism in determining cell fate following DNA damage.

Description
H2AX Polyclonal Antibody. Unconjugated. Raised in: Rabbit.

Specificity
Human, Mouse, Rat, Monkey

Isotype
IgG

Uniprot ID
P16104

Purification
Affinity Purified

Immunogen
Recombinant Protein of Human H2AFX

Storage
Shipped at 4°C. Store at -20°C. Avoid multiple freeze/thaw cycles.

Alternative Names
H2AFX, H2A.X, H2A/X, H2AX, Histone H2A.x

Application
WB, IHC, ICC

User Guide & MSDS

[User Guide]*
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