FitAmp Blood and Cultured Cell DNA Extraction Kit

The FitAmp™ Blood and Cultured Cell DNA Extraction Kit is a complete set of essential components designed for rapid isolation of pure genomic DNA. Researchers can quickly and efficiently isolate DNA from blood leukocytes and cultured mammalian cells. The entire procedure can be completed within 20 minutes.

• Fast - Entire procedure can be finished in 20 minutes with consistent isolation conditions.
• Effective - High efficiency of DNA isolation from blood leukocytes or cultured mammalian cells.
• Reliable - Specially designed F-Spin Columns allow DNA to be conveniently recovered.
• Safe - Uses non-toxic reagents and no phenol chloroform.

This kit can also be used as the initial step in our popular DNA methylation and hydroxymethylation quantitation kits: MethylFlash Global DNA Methylation 5-mC ELISA Easy Kit and MethylFlash Global DNA Hydroxymethylation 5-hmC ELISA Easy Kit.

Background
Rapid and efficient preparation of DNA is crucial to a successful DNA-based experiment. DNA samples can be prepared from cells, tissues, and body fluids, and are the most commonly used starting material for the majority of DNA methylation applications and assays. The FitAmp™ Blood and Cultured Cell DNA Extraction Kit ensures ready-to-use DNA no matter what the downstream application is.

Principle & Procedure
This kit applies a proprietary DNA isolation buffer to cell pellets. After treatment with the DNA digestion buffer, DNA is easily recovered in quantities of 10-20 µl using specially designed F-Spin Columns. DNA is then ready for downstream applications.

Starting Materials
Input amount of blood leukocytes or cultured mammalian cells should be between 1 ng and 4 µg, with the optimal range between 10 ng and 1 µg. This kit can yield 1 ng to 4 µg of DNA in spin column format, which can be used for molecular biology procedures such as PCR, restriction digestion, cloning, and sequencing.

1. Ulhe A et. al. (December 2025). Alpha-linolenic acid-mediated epigenetic reprogramming of cervical cancer cell lines. Epigenetics. 20(1):2451551.
2. Sekar J et. al. (July 2025). Metabolic imprinting drives epithelial memory during mucosal fungal infection. bioRxiv.
3. Wang J et. al. (February 2025). Targeted Delivery of α-ketoglutarate to Macrophages in Bone: A Novel Therapeutic Strategy for Improving Fracture Healing in Type 2 Diabetes. Adv Sci (Weinh). :e2415667.
4. Attia SM et. al. (July 2024). Dulaglutide reduces oxidative DNA damage and hypermethylation in the somatic cells of mice fed a high-energy diet by restoring redox balance, inflammatory responses, and DNA repair gene expressions. J Biochem Mol Toxicol. 38(7):e23764.
5. Crosswhite P et. al. (May 2024). TNFα Induces DNA and Histone Hypomethylation and Pulmonary Artery Smooth Muscle Cell Proliferation Partly via Excessive Superoxide Formation. Antioxidants (Basel). 13(6)