Cart (0)
Feng X et. al. (April 2024). Heat-Stress Impacts on Developing Bovine Oocytes: Unraveling Epigenetic Changes, Oxidative Stress, and Developmental Resilience Int J Mol Sci. 25(9)
The study investigated the effects of heat stress on bovine oocytes, revealing significant reductions in epigenetic markers, cleavage and blastocyst rates, mitochondrial function, and transzonal projections. Heat stress also increased reactive oxygen species, apoptosis, and mitochondrial autophagy. These findings indicate that heat stress impairs oocyte development by disrupting gene expression, mitochondrial function, and epigenetic modifications.
Products Used: 5-Methylcytosine (5-mC) Monoclonal Antibody [33D3], 5-Hydroxymethylcytosine (5-hmC) Monoclonal Antibody [HMC/4D9]
Song F et. al. (May 2024). Lactylome analyses suggest systematic lysine-lactylated substrates in oral squamous cell carcinoma under normoxia and hypoxia Cell Signal. :111228.
This study explores lysine lactylation (Kla) in oral squamous cell carcinoma (OSCC) under normoxia and hypoxia. Using liquid chromatography-tandem mass spectrometry (LC-MS/MS), researchers identified over 1000 Kla sites on hundreds of proteins in both conditions, with many non-histone proteins affected. Kla was enriched in pathways like spliceosome, ribosome, and glycolysis/gluconeogenesis. Under hypoxia, significant changes in Kla were observed, particularly in glycolytic proteins. Key findings included lactylation in spliceosomal proteins and a negative correlation between Kla levels and OSCC patient prognosis. This study is the first to explore the lactylome in OSCC, offering insights into tumor progression and potential therapeutic targets.
Products Used: EpiQuik Total Histone Extraction Kit
Gu Y et. al. (May 2024). Expression of genes related to gonadal development and construction of gonadal DNA methylation maps of Trachinotus blochii under hypoxia Sci Total Environ. :173172.
This article investigates how chronic hypoxia affects gonadal development in Trachinotus blochii (Golden pompano) through epigenetic changes. Hypoxia and reoxygenation disrupted the HPG axis, leading to endocrine disorders and altering the expression of key gonadal genes. Hypoxia increased the expression of dnmt and tet genes in both males and females, while reoxygenation decreased these expressions in males. DNA methylation changes were most significant on chromosomes 10 and 24, affecting genes in the oxytocin signaling, fatty acid metabolism, and HIF-1a pathways. These findings highlight the impact of hypoxia on gonadal gene expression and DNA methylation, potentially influencing gonadal development.
Products Used: MethylFlash Global DNA Methylation (5-mC) ELISA Easy Kit (Colorimetric)
Wang Y et. al. (May 2024). Comprehensive Toxicological Assessment of Halobenzoquinones in Drinking Water at Environmentally Relevant Concentration Environ Sci Technol.
This study investigates the chronic toxicity of halobenzoquinones (HBQs), a disinfection byproduct in drinking water. Using UHPLC-MS/MS, 13 HBQs were detected in water from a northern megacity in China, with four (2,6-DCBQ, 2,6-DBBQ, TriCBQ, and 2,5-DBBQ) frequently occurring. Chronic exposure of normal human colon and liver cells to these HBQs revealed that 2,5-DBBQ and 2,6-DCBQ caused significant oxidative stress and DNA damage. Additionally, these HBQs induced epithelial-mesenchymal transition (EMT) in liver cells via the ERK signaling pathway. Boiling water was found to effectively reduce HBQ levels, suggesting it as a detoxification method.
Products Used: EpiQuik 8-OHdG DNA Damage Quantification Direct Kit (Colorimetric)
McDiarmid CS et. al. (May 2024). Mitonuclear interactions impact aerobic metabolism in hybrids and may explain mitonuclear discordance in young, naturally hybridizing bird lineages Mol Ecol. :e17374.
This article examines how mitonuclear interactions affect aerobic metabolism in hybrid birds and their role in mitonuclear discordance in naturally hybridizing bird lineages. Controlled hybrid crosses showed that hybrids with mismatched mitonuclear combinations had different respiration capacities. In the wild, the mitochondrial cline center shifted west of the nuclear cline center, consistent with these findings. This suggests mitonuclear interactions impact mitochondrial respiration and may explain geographic discordance between mitochondrial and nuclear genomes in hybrid zones.
Products Used: EpiQuik 8-OHdG DNA Damage Quantification Direct Kit (Colorimetric)
Kang K et. al. (May 2024). N6-methyladenosine modification of KLF2 may contribute to endothelial-to-mesenchymal transition in pulmonary hypertension Cell Mol Biol Lett. 29(1):69.
This study investigates how N6-methyladenosine (m6A) modification of KLF2 may contribute to endothelial-to-mesenchymal transition (EndMT) in pulmonary hypertension (PH). In human pulmonary artery endothelial cells (hPAECs), reduced m6A and METTL3 levels were linked to EndMT, marked by decreased endothelial markers and increased mesenchymal markers. Inhibiting METTL3 promoted EndMT and worsened vascular remodeling and PH in mice. The study identified that METTL3-mediated m6A modification of KLF2 is crucial for mitigating EndMT. KLF2 overexpression countered EndMT effects, while mutations in its m6A site reduced its protective role. This highlights the METTL3/KLF2 pathway as a potential therapeutic target in PH.
Products Used: EpiQuik m6A RNA Methylation Quantification Kit (Colorimetric)
Huang Y et. al. (April 2024). Verbascoside inhibits oral squamous cell carcinoma cell proliferation, migration, and invasion by the methyltransferase 3-mediated microRNA-31-5p/homeodomain interacting protein kinase 2 axis Arch Oral Biol. 164:105979.
This study explores how verbascoside impacts oral squamous cell carcinoma (OSCC) through the METTL3-mediated miR-31-5p/HIPK2 axis. In OSCC cell lines SCC9 and UM1, verbascoside treatment inhibited proliferation, migration, and invasion. METTL3, which was upregulated in OSCC cells, was suppressed by verbascoside, leading to reduced cell proliferation. METTL3 facilitated miR-31-5p processing in an m6A-dependent manner, and miR-31-5p targeted HIPK2. Overexpression of miR-31-5p or HIPK2 knockdown reversed verbascoside's inhibitory effects. These findings suggest verbascoside as a potential therapeutic for OSCC by targeting the METTL3/miR-31-5p/HIPK2 pathway.
Products Used: EpiQuik m6A RNA Methylation Quantification Kit (Colorimetric)
Sun X et. al. (April 2024). Roseburia intestinalis Supplementation Could Reverse the Learning and Memory Impairment and m6A Methylation Modification Decrease Caused by 27-Hydroxycholesterol in Mice Nutrients. 16(9)
This article investigates the impact of Roseburia intestinalis supplementation on learning, memory, and N6-methyladenosine (m6A) methylation in mice treated with 27-Hydroxycholesterol (27-OHC), a factor in Alzheimer's disease. 27-OHC disrupts the gut barrier, alters m6A methylation-related enzyme expression, reduces m6A methylation levels in the brain cortex, and impairs memory. However, supplementation with Roseburia intestinalis reverses these effects, suggesting its potential as a neuroprotective agent against 27-OHC-induced cognitive impairment.
Products Used: EpiQuik m6A RNA Methylation Quantification Kit (Colorimetric)
Wang S et. al. (May 2024). ALKBH5-mediated m6A modification of circFOXP1 promotes gastric cancer progression by regulating SOX4 expression and sponging miR-338-3p Commun Biol. 7(1):565.
This study explores the role of circFOXP1, a circular RNA, in gastric cancer (GC) progression. Researchers found that circFOXP1 is overexpressed in GC tissues and is associated with poor prognosis. Functional experiments showed that circFOXP1 promotes cell proliferation, invasion, and cell cycle progression in GC. Further investigation revealed that ALKBH5 mediates m6A modification of circFOXP1, which regulates SOX4 expression and acts as a sponge for miR-338-3p. These findings suggest that circFOXP1 could be a potential biomarker and therapeutic target for GC.
Products Used: EpiQuik CUT&RUN m6A RNA Enrichment (MeRIP) Kit
Wang K et. al. (May 2024). XIST and MUC1-C form an auto-regulatory pathway in driving cancer progression Cell Death Dis. 15(5):330.
The study uncovers an auto-regulatory pathway involving the long non-coding RNA X-inactive specific transcript (XIST) and the MUC1 gene in cancer progression. MUC1-C, a subunit of the MUC1 gene, regulates XIST levels by suppressing components of the m6A methylation complex and the YTHDF2-CNOT1 deadenylase complex, leading to increased XIST stability and expression. In turn, XIST promotes MUC1-C expression through NF-κB activation. This pathway regulates genes associated with inflammation and stemness, such as miR-21 and TDP-43. TDP-43, in particular, regulates the MUC1-C/XIST pathway and drives stemness-associated genes, indicating its importance in cancer progression.
Products Used: EpiQuik CUT&RUN m6A RNA Enrichment (MeRIP) Kit
