Epigenetic Research Papers 3/17/25 - 3/21/25

Liao M et. al. (March 2025). HNRNPC promotes progression of non-small cell lung cancer by maintaining TFAP2A mRNA stability Cancer Cell Int. 25(1):85.
This study investigates the role of HNRNPC in promoting non-small cell lung cancer (NSCLC) progression by stabilizing TFAP2A mRNA through m6A modification. The findings reveal that HNRNPC enhances the TFAP2A/CTNNB1 axis, driving epithelial-mesenchymal transition (EMT) and facilitating NSCLC metastasis. These results provide new insights into the link between m6A RNA modification and tumor progression.
Products Used: N6-methyladenosine (m6A) Monoclonal Antibody [2H6], EpiQuik CUT&RUN m6A RNA Enrichment (MeRIP) Kit

Zhu Y et. al. (March 2025). Appraising histone H4 lysine 5 lactylation as a novel biomarker in breast cancer Sci Rep. 15(1):8205.
This study explores the potential of histone H4 lysine 5 lactylation (H4K5lac) as a novel biomarker in breast cancer (BC). The findings reveal that H4K5lac is significantly upregulated in BC tissues and peripheral blood mononuclear cells, correlating with lymph node involvement, Ki-67 expression, and poor overall survival. These results suggest that H4K5lac could serve as a valuable biomarker and potential target for lactylation-based therapies in BC.
Products Used: EpiQuik Total Histone Extraction Kit

Huang M et. al. (March 2025).  Apelin/APJ alleviates diabetic nephropathy by improving glomerular endothelial cells dysfunction via SIRT3‑KLF15 Mol Med Rep. 31(5)
This study examines the role of the Apelin/APJ signaling pathway in alleviating diabetic nephropathy by improving glomerular endothelial cell (GEC) function. The findings reveal that Apelin reduces glomerular basement membrane thickening and fibrosis by inhibiting extracellular matrix synthesis through the SIRT3-KLF15 pathway. These results suggest that targeting Apelin/APJ may be a potential therapeutic strategy for diabetic nephropathy.
Products Used: Epigenase Universal SIRT Activity/Inhibition Assay Kit (Colorimetric)

Yan Y et. al. (March 2025). Bacteroides fragilis Toxin Suppresses METTL3-Mediated m6A Modification in Macrophage to Promote Inflammatory Bowel Disease J Crohns Colitis. 19(3)
This study investigates how Bacteroides fragilis toxin (BFT) influences m6A RNA modification in macrophages to promote inflammatory bowel disease (IBD). The findings reveal that BFT suppresses METTL3 expression, leading to reduced m6A modifications and increased ITGA5 levels, which drive inflammation. Targeting ITGA5 with Cilengitide alleviated colitis, highlighting a potential therapeutic approach for IBD.
Products Used: EpiQuik m6A RNA Methylation Quantification Kit (Colorimetric)

Cheng Q et. al. (March 2025). FTO-mediated Nrf2 demethylation alleviates high glucose-induced oxidative stress and apoptosis in retinal pigment epithelial cells Cheng Q et. al. (March 2025). 
This study explores the role of the m6A demethylase FTO in protecting retinal pigment epithelial cells from high glucose-induced oxidative stress and apoptosis. The findings show that FTO expression is downregulated under high glucose conditions, leading to increased m6A modification and cell damage. Overexpression of FTO restores cell viability by demethylating Nrf2 mRNA, activating the antioxidant Nrf2/HO-1 pathway, suggesting a potential therapeutic target for diabetic retinopathy.
Products Used: EpiQuik m6A RNA Methylation Quantification Kit (Colorimetric)

Shi Y et. al. (March 2025). Paternal Obesity-Induced H3K27me3 Elevation Leads to MANF-Mediated Transgenerational Metabolic Dysfunction in Female Offspring Adv Sci (Weinh). :e2415956.
This study investigates how paternal obesity induces epigenetic changes in sperm, leading to elevated H3K27me3 levels that persist into embryos and cause metabolic dysfunction in female offspring. The findings reveal that increased H3K27me3 reduces MANF expression, triggering endoplasmic reticulum stress and impairing glucose metabolism. These results suggest that targeting the H3K27me3-MANF pathway could offer a potential strategy for preventing transgenerational metabolic disorders.
Products Used: EpiQuik Total Histone Extraction Kit