Epigenetic Research Papers 2/3/25 - 2/7/25

Zhao S et. al. (January 2025).  TET1 participates in oxaliplatin-induced neuropathic pain by regulating microRNA-30b/Nav1.6 J Biol Chem. :108228.
This study investigates the role of TET1 in oxaliplatin-induced neuropathic pain by regulating the microRNA-30b/Nav1.6 pathway. Findings suggest that reduced TET1 expression leads to decreased miR-30b levels, resulting in increased Nav1.6 expression and neuronal excitability, which contribute to pain. Modulating TET1 or miR-30b levels influences pain behaviors, highlighting a potential epigenetic target for neuropathic pain treatment.
Products Used: MethylFlash Global DNA Methylation (5-mC) ELISA Easy Kit (Colorimetric), MethylFlash Global DNA Hydroxymethylation (5-hmC) ELISA Easy Kit (Colorimetric), EpiQuik Hydroxymethylated DNA Immunoprecipitation (hMeDIP) Kit, ChromaFlash High-Sensitivity ChIP Kit

Koc S et. al. (January 2025).  Prepubertal phthalate exposure can cause histopathological alterations, DNA methylation and histone acetylation changes in rat brain Toxicol Ind Health. :7482337251315212.
This study examines the effects of prepubertal exposure to di-2-(ethylhexyl)phthalate (DEHP) on brain development in rats, focusing on histopathological and epigenetic changes. Findings reveal that DEHP exposure leads to neuronal loss in the hippocampus, with more severe effects at higher doses, alongside alterations in DNA methylation and histone acetylation. These results suggest that DEHP-induced epigenetic modifications may contribute to brain damage, highlighting potential developmental risks.
Products Used: EpiQuik Global Histone H3 Acetylation Assay Kit, MethylFlash Global DNA Methylation (5-mC) ELISA Easy Kit (Colorimetric)

Wang Y et. al. (January 2025). Kdm2a inhibition in skeletal muscle improves metabolic flexibility in obesity Nat Metab.
This study explores the role of Kdm2a in skeletal muscle metabolism and its impact on metabolic flexibility in obesity. Inhibiting or deleting Kdm2a enhances mitochondrial function and shifts fuel utilization from glucose to lipids, improving resistance to obesity and insulin resistance. These findings suggest that targeting Kdm2a could be a potential strategy for managing metabolic stress and obesity-related disorders.
Products Used: EpiQuik Total Histone Extraction Kit

Li Y et. al. (January 2025). Epitranscriptome profiles reveal participation of the RNA methyltransferase gene OsMTA1 in rice seed germination and salt stress response BMC Plant Biol. 25(1):115.
This study investigates the role of the RNA methyltransferase gene OsMTA1 in rice seed germination and response to salt stress. Loss of OsMTA1 reduced m6A methylation, delayed germination, and increased oxidative damage, particularly under salt stress. The findings highlight OsMTA1 as a key regulator of gene expression during germination and stress adaptation, offering potential targets for breeding stress-tolerant rice varieties.
Products Used: EpiQuik m6A RNA Methylation Quantification Kit (Colorimetric)

Lin L et. al. (January 2025). Disrupting of IGF2BP3-stabilized CLDN11 mRNA by TNF-α increases intestinal permeability in obesity-related severe acute pancreatitis Mol Med. 31(1):24.
This study explores the role of CLDN11 in regulating intestinal permeability in obesity-related severe acute pancreatitis (SAP). The findings reveal that IGF2BP3 stabilizes CLDN11 mRNA, but TNF-α disrupts this interaction, leading to increased intestinal permeability and worsened pancreatic inflammation. Restoring CLDN11 expression or inhibiting TNF-α reduced these effects, highlighting potential therapeutic targets for obesity-related SAP.
Products Used: EpiQuik m6A RNA Methylation Quantification Kit (Colorimetric)

Li Y et. al. (January 2025). Topologically constrained DNA-mediated one-pot CRISPR assay for rapid detection of viral RNA with single nucleotide resolution EBioMedicine. 112:105564.
This study investigates a one-pot CRISPR-based assay utilizing topologically constrained DNA to enable rapid and precise viral RNA detection with single-nucleotide resolution. By optimizing the Cas13a trans-cleavage system, the assay accurately identifies SARS-CoV-2 variants, including Omicron, within 30 minutes without pre-amplification. This approach offers a highly sensitive (0.1 aM), specific, and portable diagnostic tool adaptable for detecting various RNA viruses.
Products Used: EpiQuik Viral RNA Isolation Fast Kit