Epigenetic Research Papers 1/15/24 - 1/19/24

Kang SH et. al. (January 2024). Differential effect of cancer-associated fibroblast-derived extracellular vesicles on cisplatin resistance in oral squamous cell carcinoma via miR-876-3p Theranostics. 14(2):460-479.
This study investigates the impact of extracellular vesicles, derived from supportive cells in oral squamous cell carcinoma, on the tumor's resistance to cisplatin. By examining the roles of miR-876-3p, IGFBP3, and GATA1 molecules, the study aims to provide valuable insights for researchers exploring how these particles influence the cancer's response to
Products Used: EpiQuik Chromatin Immunoprecipitation (ChIP) Kit

Lv H et. al. (January 2024).  TET2-mediated tumor cGAS triggers endothelial STING activation to regulate vasculature remodeling and anti-tumor immunity in liver cancer Nat Commun. 15(1):6.
The article explores the intricate relationship between tumor cGAS and host STING in liver cancer, revealing their interdependence in mediating vascular normalization and anti-tumor immune responses. The research identifies TET2-mediated signaling and the role of vitamin C in enhancing these processes, shedding light on potential strategies to improve combinational immunotherapy efficacy for liver cancer.
Products Used: EpiQuik Chromatin Immunoprecipitation (ChIP) Kit

Wang X et. al. (January 2024). STAT3 and SOX-5 induce BRG1-mediated chromatin remodeling of RORCE2 in Th17 cells Commun Biol. 7(1):10.
This study investigates the molecular mechanisms underlying the activation of RORCE2, a critical enhancer for the RORγt gene in T helper 17 (Th17) cells. The research reveals that both STAT3 and SOX-5 contribute to the enhancer activity of RORCE2 by recruiting the chromatin remodeling factor BRG1, shedding light on the intricate regulation of Th17 cell differentiation.
Products Used: EpiQuik Chromatin Accessibility Assay Kit

Zhao X et. al. (January 2024).  Temporally-coordinated bivalent histone modifications of BCG1 enable fungal invasion and immune evasion Nat Commun. 15(1):231.
This study investigates the role of bivalent histone modifications, specifically H3K4me3 and H3K27me3, in fungal virulence using Fusarium graminearum (Fg) as a model. The research identifies infection-related bivalent chromatin-marked genes (BCGs) and reveals that the BCG1 gene, encoding a secreted xylanase, undergoes dynamic bivalent modifications during Fg invasion, allowing the fungus to temporally coordinate gene expression for efficient host cell wall degradation and subsequent immune evasion.
Products Used: Histone H3K4me1 Monomethyl Peptide Biotinylated, Histone H3K4me2 Dimethyl Peptide Biotinylated, Histone H3K4me3 Trimethyl Peptide Biotinylated, Histone H3K27me1 Monomethyl Peptide Biotinylated, Histone H3K27me2 Dimethyl Peptide Biotinylated, Histone H3K27me3 Trimethyl Peptide Biotinylated

Boada MD et. al. (January 2024). Effects of systemic oxytocin administration on UVB-induced nociceptive hypersensitivity and tactile hyposensitivity in mice Mol Pain. :17448069241226553.
This study explores the impact of UVB radiation on cutaneous inflammation, specifically its effects on mechanical properties and function of tactile and nociceptive peripheral afferents in mice. The research reveals that oxytocin (OXT) administration can modulate these disruptions, highlighting its potential role in mitigating UVB-induced nociceptive hypersensitivity and tactile hyposensitivity.
Products Used: Avpr1a Polyclonal Antibody

Kim J et. al. (January 2024). Differential DNA methylation and metabolite profiling of Atlantic killifish (Fundulus heteroclitus) from the New Bedford Harbor Superfund site Ecotoxicology.
The article investigates the interplay between genetic and environmental factors in Atlantic killifish populations from the New Bedford Harbor Superfund site, known for its polychlorinated biphenyl (PCB) contamination. The findings reveal tissue- and sex-specific global DNA hypomethylation in the liver of killifish from the contaminated site, along with differential metabolites, providing insights into the epigenetic and metabolic adaptations of these fish to environmental pollution.
Products Used: MethylFlash Global DNA Methylation (5-mC) ELISA Easy Kit (Colorimetric)

Song M et. al. (January 2024). N6 methyladenosine eraser FTO suppresses Staphylococcus aureus-induced ferroptosis of bone marrow mesenchymal stem cells to ameliorate osteomyelitis through regulating the MDM2/TLR4/SLC7A11 signaling pathway Song M et. al. (January 2024).
This study delves into the role of ferroptosis in Staphylococcus aureus (SA)-induced osteomyelitis, a bone-destructive inflammatory disease. The research reveals that SA induces ferroptosis in bone marrow mesenchymal stem cells (BMSCs) through the MDM2/TLR4/SLC7A11 signaling pathway, and upregulation of the N6 methyladenosine eraser FTO protects against SA-triggered ferroptosis in BMSCs, providing insights into potential therapeutic targets for osteomyelitis.
Products Used: EpiQuik m6A RNA Methylation Quantification Kit (Colorimetric)

Cai B et. al. (January 2024). MYH1G-AS is a chromatin-associated lncRNA that regulates skeletal muscle development in chicken Cai B et. al. (January 2024).
The article explores the regulatory role of the chromatin-associated RNA MYH1G-AS in skeletal muscle development in chickens. The research uncovers a mechanism involving transcription factors SMAD3 and SP2, ALKBH5-mediated m6A demethylation, and the influence of MYH1G-AS on myoblast proliferation, differentiation, fiber type switching, and muscle atrophy, providing insights into the complex regulatory network governing skeletal muscle development.
Products Used: EpiQuik m6A RNA Methylation Quantification Kit (Colorimetric)

Li S et. al. (January 2024). Dietary selenomethionine reduced oxidative stress by resisting METTL3-mediated m6A methylation level of Nrf2 to ameliorate LPS-induced liver necroptosis in laying hens Biochem. 125:109563.
This study explores the protective mechanism of dietary selenomethionine (SeMet) against liver injury in laying hens induced by lipopolysaccharide (LPS). The research reveals that SeMet reduces LPS-induced oxidative stress by suppressing total m6A methylation levels and METTL3 expression, specifically decreasing the m6A methylation level of Nrf2, activating antioxidant pathways, and mitigating liver damage and inflammation.
Products Used: EpiQuik m6A RNA Methylation Quantification Kit (Colorimetric)

Gao L et. al. (January 2024). ALKBH5 regulates paclitaxel resistance in NSCLC via inhibiting CEMIP-mediated EMT Toxicol Appl Pharmacol. :116807.
The study investigates the role of ALKBH5, an m6A demethylase, in non-small cell lung cancer (NSCLC) and its resistance to paclitaxel (PTX). Demonstrating a tumor-suppressive function, ALKBH5 overexpression enhances chemo-sensitivity, counteracts the epithelial-mesenchymal transition (EMT) process in PTX-resistant cells, and achieves this by negatively regulating CEMIP (cell migration inducing hyaluronidase 1) through mRNA destabilization.
Products Used: EpiQuik m6A RNA Methylation Quantification Kit (Colorimetric)

Yu T et. al. (January 2024).  Effects of methionine deficiency on B7H3-DAP12-CAR-T cells in the treatment of lung squamous cell carcinoma Cell Death Dis. 15(1):12.
In the study addressing lung squamous cell carcinoma (LUSC), the researchers developed B7H3-DAP12-CAR-T cells to target the highly expressed B7H3 protein in LUSC. However, the tumor microenvironment's competition for methionine led to Met deficiency, compromising CAR-T function and underscoring the need to consider amino acid metabolism for improving CAR-T efficacy in solid tumor microenvironments.
Products Used: MethylFlash 5-mC RNA Methylation ELISA Easy Kit (Fluorometric)

Brown EJ et. al. (January 2024). PRMT5 inhibition shows in vitro efficacy against H3K27M-altered diffuse midline glioma, but does not extend survival in vivo Sci Rep. 14(1):328.
In the study focused on H3K27M-altered Diffuse Midline Glioma (DMG), researchers screened an epigenetic inhibitor library and identified protein arginine methyltransferase (PRMT) inhibitors, LLY-283 and GSK591, as top hits in reducing DMG cell viability. Despite LLY-283 showing efficacy in vitro by targeting PRMT5, it did not extend survival in an orthotopic xenograft model, underscoring the challenges in developing effective treatments for DMG.
Products Used: Histone H4R3 Dimethyl Symmetric (H4R3me2s) Polyclonal Antibody

Rubas NC et. al. (January 2024).  HMGB1 mediates microbiome-immune axis dysregulation underlying reduced neutralization capacity in obesity-related post-acute sequelae of SARS-CoV-2 Sci Rep. 14(1):355.
This study explores how dysregulation in the microbiome-immune axis, specifically involving the HMGB1 protein, contributes to the reduced neutralization capacity observed in post-acute sequelae of SARS-CoV-2 (PASC), particularly in individuals with obesity. The findings suggest HMGB1 as a potential biomarker for PASC and a target for therapeutic interventions.
Products Used: SeroFlash SARS-CoV-2 IgG/IgM ELISA Fast Kit