The SeroFlash™ SARS-CoV-2 IgG/IgM ELISA Fast Kit is a complete set of optimized buffers and reagents to qualitatively detect the IgG and IgM antibodies of the novel coronavirus, SARS-CoV-2, from serum or plasma within 45 min.
The kit has the following advantages:
Intended UseThis product is used for qualitatively identifying IgM and IgG antibodies against SARS-CoV-2 in human serum and plasma. It is intended for research scientists only. This product is not to be used for screening donated serum or plasma.
BackgroundIn the process of pathogenic microorganism infection, IgG and IgM are the most commonly used antibody markers of infectious diseases. IgM, as the first antibody in the process of infection, is usually used as a marker of acute infection. With the development of infection, IgM concentration gradually decreases and disappears after the appearance of IgG. IgG usually exists in the body for a long time, even if the virus has been completely eliminated. Serum and plasma obtained from positive blood can be used as an indicator of infection and previous infection. Therefore, detecting SARS-CoV-2 IgG and IgM antibodies is of great clinical significance and could effectively control large-scale transmission of SARS-CoV-2.
Principles of the TechnologyThis kit uses an indirect ELISA technology to qualitatively measure the SARS-CoV-2 IgG and IgM in serum and plasma. The assay plate is coated with a recombinant SARS-CoV-2 antigen (Spike protein). In the assay, samples are added into the plate wells. An immuno-complex bound in the well will be formed by the coated-SARS-CoV-2 antigen and the SARS-CoV-2 IgG/IgM antibodies contained in the sample. Such immuno-complex will be recognized by the HRP-conjugated anti-human IgG or IgM antibody. The color will be generated by the interaction of the bound immune-complex with TMB substrate. The more SARS-CoV-2 IgG or IgM antibody there is, the stronger the blue coloration. The reaction is stopped with stop buffer, which turns the solution yellow. The signal intensity in the wells can then be read, spectrophotometrically using a microplate reader at a wavelength of 450 nm.
Starting MaterialsThis assay is suitable for human serum and plasma.