Epigenetic Research Papers 11/27/23 - 12/1/23

Kmiołek T et. al. (November 2023). Aging and the impact of global DNA methylation, telomere shortening, and total oxidative status on sarcopenia and frailty syndrome Immun Ageing. 20(1):61.
This study investigates the impact of aging on global DNA methylation, telomere shortening, and total oxidative status in relation to sarcopenia and frailty syndrome. The research emphasizes age as a primary factor influencing methylation, telomere length, and oxidative stress levels. These findings provide insight into the underlying mechanisms associated with age-related conditions.
Products Used: MethylFlash Global DNA Methylation (5-mC) ELISA Easy Kit (Colorimetric)

Wu Y et. al. (November 2023). Effects of Methionine Restriction from Different Sources on Sperm Quality in Aging Mice Nutrients. 15(22)
In this study, researchers investigated the impact of methionine restriction on sperm quality in aging mice. The results revealed that mice subjected to methionine restriction, particularly those receiving HMTBA, showed enhanced sperm quality, reduced testicular inflammation and oxidative stress, along with altered methylation patterns in sperm RNA and DNA, suggesting potential nutritional strategies to alleviate age-related decline in sperm quality.
Products Used: MethylFlash Methylated DNA 5-mC Quantification Kit (Colorimetric)

Wang Z et. al. (November 2023). TET1-mediated epigenetic regulation of tumor necrosis factor-α in trigeminal ganglia contributes to chronic temporomandibular joint pain Life Sci. :122283.
The article explores the epigenetic regulation of tumor necrosis factor-α (TNFα) in trigeminal ganglia (TG) associated with chronic temporomandibular joint (TMJ) pain. Employing a chronic TMJ pain mouse model, the study revealed increased hydroxymethylation at the TNFα promoter region in TG and spinal trigeminal nucleus caudalis (Sp5C), with TET1 upregulation and direct binding to the TNFα promoter. The findings indicate TET1-mediated epigenetic modulation in the pathogenesis of chronic TMJ pain.
Products Used: EpiQuik Hydroxymethylated DNA Immunoprecipitation (hMeDIP) Kit, 5-Hydroxymethylcytosine (5-hmC) Monoclonal Antibody [HMC/4D9]

Rowbotham SP et. al. (November 2023). Age-associated H3K9me2 loss alters the regenerative equilibrium between murine lung alveolar and bronchiolar progenitors Dev Cell.
In this article, researchers investigate the role of histone H3 lysine 9 di-methylation (H3K9me2) in regulating distal lung epithelial progenitor cells during injury repair and how this regulation changes with age. The study reveals that H3K9me2 loss in aged mouse lungs is associated with decreased alveolar type 2 (AT2) cell progenitors, reduced alveolar regeneration, and increased activity of multipotent bronchioalveolar stem cells (BASCs) and bronchiolar progenitor club cells. This dysregulation in epigenetic control during aging contributes to a better understanding of age-related susceptibility to lung diseases.
Products Used: 5-Methylcytosine (5-mC) Monoclonal Antibody [33D3]

Kovatcheva M et. al. (November 2023).  Vitamin B12 is a limiting factor for induced cellular plasticity and tissue repair Nat Metab. 5(11):1911-1930.
This study investigates the metabolic requirements of induced cellular plasticity and tissue repair through transient reprogramming with OCT4, SOX2, KLF4, and MYC (OSKM). The research revealed that OSKM reprogramming depletes vitamin B12 levels, and supplementation with vitamin B12 enhances reprogramming efficiency in mice and cultured cells, suggesting a cell-intrinsic effect.
Products Used: EpiQuik Histone H3 Modification Multiplex Assay Kit (Colorimetric), EpiQuik Total Histone Extraction Kit

Guo X et. al. (November 2023). Neuronal activity promotes glioma progression by inducing proneural-to-mesenchymal transition in glioma stem cells Cancer Res.
This article investigates the impact of neuronal activity on glioblastoma progression, revealing that neuronal activity promotes glioma stem cell (GSC) progression and radioresistance through exosome-induced proneural-to-mesenchymal transition (PMT). Neuronal activation led to increased miR-184-3p in neuron-derived exosomes, influencing GSCs by reducing mRNA N6-methyladenosine (m6A) levels and activating the STAT3 pathway, ultimately contributing to GSC PMT.
Products Used: EpiQuik m6A RNA Methylation Quantification Kit (Colorimetric)

Wang Z et. al. (November 2023). Up-regulation of RNA m6A methyltransferase like-3 expression contributes to arsenic and benzo[a]pyrene co-exposure-induced cancer stem cell-like property and tumorigenesis Toxicol Appl Pharmacol. 481:116764.
In this study, the impact of arsenic and benzo[a]pyrene (BaP) co-exposure on lung tumorigenic effects was investigated. Arsenic plus BaP co-exposure was found to significantly up-regulate RNA N6-methyladenosine (m6A) methylation levels, with a specific focus on the m6A writer methyltransferase like-3 (METTL3), contributing to cancer stem cell characteristics and tumorigenesis.
Products Used: EpiQuik m6A RNA Methylation Quantification Kit (Colorimetric)

Xu Y et. al. (November 2023). Increased m6A-RNA methylation and demethylase FTO suppression is associated with silica-induced pulmonary inflammation and fibrosis Toxicology. 500:153673.
The article explores the involvement of N6-methyladenosine (m6A) RNA modification in the early phase of silica-induced pulmonary inflammation and fibrosis, a severe occupational hazard. The study reveals an upregulation of global m6A modification levels and the suppression of the demethylase FTO, suggesting potential diagnostic and therapeutic implications for silicosis.
Products Used: EpiQuik m6A RNA Methylation Quantification Kit (Colorimetric)

Wang YZ et. al. (November 2023). Discovery of Pyrazolo[1,5- a]pyrimidine Derivative as a Novel and Selective ALKBH5 Inhibitor for the Treatment of AML J Med Chem.
The study explores DDO-2728 as a selective inhibitor of ALKBH5, a key enzyme involved in the demethylation of m6A (N6-methyladenosine). DDO-2728 demonstrated the ability to selectively inhibit ALKBH5, increased m6A modifications in AML cells, and exhibited anti-tumor effects in a xenograft mouse model, suggesting its potential for targeted therapies in acute myeloid leukemia (AML).
Products Used: EpiQuik m6A RNA Methylation Quantification Kit (Colorimetric)