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E2F4 Monoclonal Antibody [5B7]

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Application: ELISA, WB
Areas of Research: Transcription Factors
Clonality: Monoclonal
Conjugate: Unconjugated
Host: Mouse
Isotype: IgG1 Kappa
Purification: Affinity Purified
Reactivity: Human
100% Guarantee: 6 months
Catalog No.SizePriceQty
A-2206-100100 µg $452.00  Discontinued
Availability: Discontinued 
Product Overview

Description
Mouse monoclonal antibody raised against a partial recombinant E2F4, clone 5B7.

Size
100 µg

Purification
Protein A purified

Immunogen
E2F4 (NP_001941, 211 a.a. ~ 301 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Cross Reactivity
Human

Isotype
IgG1 Kappa

Formulation
In 1x PBS, pH 7.2

Storage
Aliquot and store at -20ºC or lower. Avoid repeated freezing and thawing. Multiple freeze/thaw cycles may result in decreased performance.

Handling Recommendations
For maximum recovery of the products, centrifuge the vial prior to opening the cap.

Alternative Names
p107/p130 binding protein antibody, Transcription factor E2F4 antibody

Applications & Suggested Dilutions*
Western Blot: 0.5-1 µg/ml
ELISA: 0.25-1 µg/ml

*The end user is responsible for determining optimal working dilutions.

Quality Control

Western Blot detection against Immunogen (36.01 KDa).

 

Note: This item was formerly catalog no. ABB-2223 (ABB2223)

Product Details

Applications

Western Blot


E2F4 Monoclonal Antibody, clone 5B7. Western Blot analysis of E2F4 expression in A-549.
Immunoprecipitation Not Determined

ELISA


Detection limit for recombinant GST tagged E2F4 is approximately 0.03 ng/ml as a capture antibody.

Immunohistochemistry (IHC)

Not Determined

Immunofluorescence

Not Determined
User Guide & MSDS

[User Guide]*
*Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing info@epigentek.com along with your contact information and institution name.

Introduction to Epigenetics

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