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DPPA5 Polyclonal Antibody

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Western Blot
Positive WB detected in: Mouse kidney tissue
All lanes: DPPA5 antibody at 4.6ug/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 14 KDa
Observed band size: 14 KDa
IHC image of DPPA5 Polyclonal Antibody diluted at 1:200 and staining in paraffin-embedded human liver cancer performed on a Leica Bond™ system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image of DPPA5 Polyclonal Antibody diluted at 1:200 and staining in paraffin-embedded human ovarian cancer performed on a Leica Bond™ system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of HepG2 cells with DPPA5 Polyclonal Antibody at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
Application: ELISA, IF, IHC, WB
Areas of Research: Cell Development & Differentiation
Clonality: Polyclonal
Conjugate: Unconjugated
Host: Rabbit
Isotype: IgG
Purification: Protein G Purified
Reactivity: Human, Mouse
Trial Size Available: Yes
100% Guarantee: 6 months
Catalog No.SizePriceQty
A69467-02020 µg $99.00 
A69467-05050 µg $249.00 
A69467-100100 µg $399.00 
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Product Overview

Background
Involved in the maintenance of embryonic stem (ES) cell pluripotency. Dispensable for self-renewal of pluripotent ES cells and establishment of germ cells. Associates with specific target mRNAs.

Description
DPPA5 Polyclonal Antibody. Unconjugated. Raised in: Rabbit.

Formulation
Liquid. 0.03% Proclin 300. 50% Glycerol, 0.01M PBS, pH 7.4.

Specificity
Human, Mouse

Isotype
IgG

Uniprot ID
A6NC42

Purification
Protein G purified

Immunogen
Recombinant Human Developmental pluripotency-associated 5 protein (1-116AA)

Storage
Shipped at 4°C. Upon receipt, store at -20°C (short-term) or -80°C (long-term). Avoid repeated freeze.

Alternative Names
Developmental pluripotency-associated 5 protein, hDPPA5, Embryonal stem cell-specific gene 1 protein, ESG-1, DPPA5, ESG1

Application
ELISA, WB, IHC, IF; Recommended dilution: IHC:1:200-1:500, IF:1:50-1:200

User Guide & MSDS

[User Guide]*
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