DPPA5 Polyclonal Antibody

▲

▼

Western Blot
Positive WB detected in: Mouse kidney tissue
All lanes: DPPA5 antibody at 4.6ug/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 14 KDa
Observed band size: 14 KDa

IHC image of DPPA5 Polyclonal Antibody diluted at 1:200 and staining in paraffin-embedded human liver cancer performed on a Leica Bond™ system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

IHC image of DPPA5 Polyclonal Antibody diluted at 1:200 and staining in paraffin-embedded human ovarian cancer performed on a Leica Bond™ system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunofluorescence staining of HepG2 cells with DPPA5 Polyclonal Antibody at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).

Application:	ELISA, IF, IHC, WB
Areas of Research:	Cell Development & Differentiation
Clonality:	Polyclonal
Conjugate:	Unconjugated
Host:	Rabbit
Isotype:	IgG
Purification:	Protein G Purified
Reactivity:	Human, Mouse
100% Guarantee:	6 months

Catalog No.	Size	Price	Qty
A69467-020	20 µl	Discontinued		Discontinued
A69467-050	50 µl	Discontinued		Discontinued
A69467-100	100 µl	$445.00

Request A Bulk Quote

Buy one get one free conjugated antibodies

Earn up to 445 reward points for this purchase!

Order now & get it by Tuesday, October 15th

Product Overview
Datasheet & SDS
Product FAQ

Product Overview

Background
Involved in the maintenance of embryonic stem (ES) cell pluripotency. Dispensable for self-renewal of pluripotent ES cells and establishment of germ cells. Associates with specific target mRNAs.

Description
DPPA5 Polyclonal Antibody. Unconjugated. Raised in: Rabbit.

Formulation
0.03% Proclin 300. 50% Glycerol, 0.01M PBS, pH 7.4.

Specificity
Human, Mouse

Isotype
IgG

Uniprot ID
A6NC42

Purification
Protein G purified

Immunogen
Recombinant Human Developmental pluripotency-associated 5 protein (1-116AA)

Storage
Shipped at 4°C. Upon receipt, store at -20°C (short-term) or -80°C (long-term). Avoid repeated freeze.

Alternative Names
Developmental pluripotency-associated 5 protein, hDPPA5, Embryonal stem cell-specific gene 1 protein, ESG-1, DPPA5, ESG1

Application
ELISA, WB, IHC, IF; Recommended dilution: WB:1:500-1:5000, IHC:1:200-1:500, IF:1:50-1:200

Datasheet & SDS

Datasheet/User Guide
If the above file is not available online, you can request a copy by contacting us.

Product FAQ

No FAQs found at this time

Browse By Category

Products

Antibodies

Primary Antibodies

Cell Structure & Function Antibodies

Chromatin & Transcription Antibodies

Histone Modification Antibodies

Signal Transduction Antibodies

Metabolism Antibodies

Immunology & Inflammation Antibodies

Antibody Panel Packs

Instruments

Research Kits

DNA Methylation

Chromatin & Transcription

Histone Methylation

Acetylation & Deacetylation

Sample Preparation

Other Histone Modifications

Proteins & Enzymes

DNA Methylation/Demethylation Proteins

Modified Histone Proteins

Peptides

COVID-19 Assays

Services

DNA-based NGS Services

Chromatin-based NGS Services

qPCR-based Services

ELISA-based Services

Support

Technical Support

Quality Assurance

Resources

Corporate

Company Information

Procurement

Communication

DPPA5 Polyclonal Antibody