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CTCF Polyclonal Antibody

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Western blot analysis of extracts of various cell lines, using CTCF antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded human colon using CTCF antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat spleen using CTCF antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse spleen using CTCF antibody at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using CTCF antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Confocal immunofluorescence analysis of Hela cells using CTCF Polyclonal Antibody at dilution of 1:200. Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3ug CTCF antibody. Western blot was performed from the immunoprecipitate using CTCF antibody at a dilition of 1:1000.
Chromatin immunoprecipitation analysis of extracts of HCT116 cells, using CTCF antibody and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
Application: ChIP, IF, IHC, IP, WB
Areas of Research: Chromatin Remodeling
Clonality: Polyclonal
Conjugate: Unconjugated
Host: Rabbit
Isotype: IgG
Purification: Affinity Purified
Reactivity: Human, Monkey, Mouse, Rat
100% Guarantee: 6 months
Catalog No.SizePriceQty
A-2741-05050 µl $189.00 
A-2741-100100 µl $299.00 
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Product Overview

Background
Highly conserved transcription factors CTCF (CCCTC-binding factor) and its paralog (BORIS) regulate transcriptional activation and repression, insulator function, and imprinting control regions (ICRs). Both CTCF and BORIS contain 11 conserved zinc finger domains that work together to bind the same DNA elements, even though they have different amino and carboxy termini. CTCF is ubiquitously expressed and influences transcriptional regulation of cell-growth regulated genes. It is also necessary for the enhancer-blocking activity of all known insulator elements and ICRs. CTCF DNA-binding is sensitive to DNA methylation, a mark that determines parent-of-origin selection of the imprinted allele. CTCF functions depend on interactions with various proteins and posttranslational modifications, such as phosphorylation, SUMOylation and poly(ADP-ribosyl)ation. In particular, poly(ADP-ribosyl)ation has been found to regulate CTCF insulator and chromatin barrier functions and may also affects the transcription of rRNA. Mutations in this gene have been associated with aggressive breast cancers, prostate cancers, and Wilms' tumors.

Description
CTCF Polyclonal Antibody. Unconjugated. Raised in: Rabbit.

Formulation
Liquid. PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Specificity
Human, Mouse, Rat, Monkey

Isotype
IgG

Uniprot ID
P49711

Purification
Affinity Purified

Immunogen
Recombinant Protein of Human CTCF

Storage
Shipped at 4°C. Store at -20°C. Avoid multiple freeze/thaw cycles.

Application
WB, IHC, IF, IP, ChIP; Recommended dilution: WB 1:500 - 1:2000, IHC 1:50 - 1:200, IF 1:50 - 1:200, IP 1:50 - 1:100, ChIP 1:50 - 1:200

User Guide & MSDS

[User Guide]*
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