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CTCF Polyclonal Antibody

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All lanes: CTCF Polyclonal Antibody at 6ug/ml
Lane 1: PC-3 whole cell lysate
Lane 1: MCF-7 whole cell lysate
Secondary
Goat polyclonal to rabbit at 1/10000 dilution
Predicted band size: 83, 46 kDa
Observed band size: 83 kDa
Immunohistochemistry of paraffin-embedded human ovarian cancer at dilution 1:100
Chromatin Immunoprecipitation Hela(1.2*106) were cross-linked with formaldehyde, sonicated, and immunoprecipitated with 4ug anti-CTCF or a control normal rabbit IgG . The resulting ChIP DNA was quantified using real-time PCR with primers against the H19ICR promoter.
Application: ChIP, ELISA, IHC
Areas of Research: Chromatin Remodeling
Clonality: Polyclonal
Conjugate: Unconjugated
Host: Rabbit
Isotype: IgG
Purification: Affinity Purified
Reactivity: Human
100% Guarantee: 6 months
Catalog No.SizePriceQty
A68302-05050 µl $199.00 
A68302-100100 µl $299.00 
Availability: Usually Ships Within 1-2 Weeks 
Product Overview

Background
Chromatin binding factor that binds to DNA sequence specific sites. Involved in transcriptional regulation by binding to chromatin insulators and preventing interaction between promoter and nearby enhancers and silencers. Acts as transcriptional repressor binding to promoters of vertebrate MYC gene and BAG1 gene. Also binds to the PLK and PIM1 promoters. Acts as a transcriptional activator of APP. Regulates APOA1/C3/A4/A5 gene cluster and controls MHC class II gene expression. Plays an essential role in oocyte and preimplantation embryo development by activating or repressing transcription. Seems to act as tumor suppressor. Plays a critical role in the epigenetic regulation. Participates in the allele-specific gene expression at the imprinted IGF2/H19 gene locus. On the maternal allele, binding within the H19 imprinting control region (ICR) mediates maternally inherited higher-order chromatin conformation to restrict enhancer access to IGF2. Plays a critical role in gene silencing over considerable distances in the genome. Preferentially interacts with unmethylated DNA, preventing spreading of CpG methylation and maintaining methylation-free zones. Inversely, binding to target sites is prevented by CpG methylation. Plays a important role in chromatin remodeling. Can dimerize when it is bound to different DNA sequences, mediating long-range chromatin looping. Mediates interchromosomal association between IGF2/H19 and WSB1/NF1 and may direct distant DNA segments to a common transcription factory. Causes local loss of histone acetylation and gain of histone methylation in the beta-globin locus, without affecting transcription. When bound to chromatin, it provides an anchor point for nucleosomes positioning. Seems to be essential for homologous X-chromosome pairing. May participate with Tsix in establishing a regulatable epigenetic switch for X chromosome inactivation. May play a role in preventing the propagation of stable methylation at the escape genes from X- inactivation. Involved in sister chromatid cohesion. Associates with both centromeres and chromosomal arms during metaphase and required for cohesin localization to CTCF sites. Regulates asynchronous replication of IGF2/H19.

Description
CTCF Polyclonal Antibody. Unconjugated. Raised in: Rabbit.

Formulation
Liquid. PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Specificity
Human

Isotype
IgG

Uniprot ID
P49711

Purification
Affinity purified

Immunogen
Recombinant Human Transcriptional repressor CTCF protein (1-260AA)

Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C (short-term) or -80°C (long-term). Avoid repeated freeze.

Alternative Names
11-zinc finger protein, CCCTC-binding factor, CTCFL paralog, CTCF,

Application
ELISA, IHC, ChIP; Recommended dilution: IHC:1:20-1:200

User Guide & MSDS

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